Hektoen Enteric Agar Plates/W Tetracyline
Catalog.No: DCP-HEAPT
Features:
Uses: Isolation and differentiation of Salmonella and Shigella from food, water, and environmental samples in food safety and public health labs, and fecal specimens in clinical diagnostics
pH: 7.5 ± 0.2
Size: 100 mm
Concentration: 20µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: green with a yellowish cast; clear to slightly opalescent gel.
Sterile
Shelf Life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Hektoen Enteric Agar (HE) with tetracycline is a modified moderately selective and differential plate medium in which the standard HE formulation is supplemented with a broad-spectrum protein synthesis inhibitor that further suppresses tetracyline-susceptible background flora. HE contains bile salts and dyes that selectively inhibit most Gram-positive organisms. Most non-pathogenic coliform flora are partially or fully inhibited, and this allows enteric pathogens that are able to tolerate the bile and dyes to grow more readily. HE also contains carbohydrates, and fermenting non-target enterics produce acid shifting colony and medium color to yellow to orange/salmon around the colonies. With carbohydrate fermentation salmonella and shigella act as non-fermenters relying on peptone and as a result, they produce alkaline products that make the colony and surrounding medium green to blue-green. Sodium thiosulfate and ferric ammonium citrate help detect H2S by forming black ferrous sulfide, Many Salmonella will have black centers in their colonies.
Tetracycline is a broad-spectrum bacteriostatic antibiotic that binds to the 30S ribosomal subunit and blocks aminoacyl-tRNA binding, inhibiting protein synthesis in susceptible bacteria. It inhibits many Gram-positive and Gram-negative organisms, including numerous enteric commensals. The combination of HE and tetracycline allow for the further suppression of tetracycline-susceptible Gram-negative rods and Gram-positives that might otherwise grow despite bile and dyes, enriching for tetracycline-resistant Salmonella, Shigella, or engineered enteric strains. This reduces background growth and swarming, improving the detection and isolation of resistant target colonies.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
