DNA Marker (500-15000 bp) with TBE & Loading Dye
DNA Marker (500-15000 bp) with TBE & Loading Dye
Ready-to-load DNA marker with nine bands from 500–15,000 bp, supplied with Loading Buffer [10X] and TBE Buffer [10X] for agarose gel electrophoresis.
For Research Use Only.
Overview
A wide-range ladder for large DNA fragments, with the buffers to run it.
This kit provides the three essential reagents for routine agarose gel electrophoresis of DNA fragments in the 500–15,000 bp range. It is built for work with large fragments: long-range PCR products, plasmid and genomic DNA, and restriction digests that produce multi-kilobase bands. The DNA marker contains nine linear double-stranded DNA fragments at 500, 1000, 1500, 2000, 3000, 5000, 7500, 10000 and 15000 bp.
The marker also comes premixed with loading buffer, so you can load it directly with no mixing or extra pipetting. The electrophoresis bands are clear, facilitating accurate determination of the DNA content of the target band. When in use, take 5-10 µL and it can be directly used for gel electrophoresis. Paired with a concentrated TBE buffer [10X] and a Gel Loading Buffer [10X] for your samples, it takes you from sample to sized bands quickly and consistently.
Each kit contains a tube of Loading Buffer [10X]. Combine the loading buffer with the DNA sample to achieve a 1X final concentration, then mix thoroughly and load the sample for agarose gel electrophoresis.
Band Pattern & Gel Image
Within the DNA Marker, the 2000 bp fragment is intentionally enhanced for easy identification and produces a brighter band. This fragment contains 100 ng DNA per 5 µL, whereas each of the remaining DNA fragments contains 50 ng per 5 µL.
| Fragment | DNA per 5 µL |
|---|---|
| 500 bp | 50 ng |
| 1000 bp | 50 ng |
| 1500 bp | 50 ng |
| 2000 bpReference band | 100 ng |
| 3000 bp | 50 ng |
| 5000 bp | 50 ng |
| 7500 bp | 50 ng |
| 10000 bp | 50 ng |
| 15000 bp | 50 ng |
Features & Applications
Ready-to-load marker
Premixed with loading buffer, so you pipette it directly into the well. This saves a step and removes a common source of pipetting error.
Wide sizing range in one lane
A single ladder covers 500 bp to 15 kb, so one marker serves long PCR, digest mapping and DNA integrity checks.
Sharp bands, stable runs
TBE has greater buffering capacity than TAE and heats less, so it holds pH through long runs and tolerates higher voltages without band distortion.
Room-temperature buffer stock
The 10X TBE concentrate needs no cold storage, saving freezer space.
Applications
- Sizing long-range and standard PCR products
- Restriction digest and plasmid mapping
- Checking genomic DNA and high-molecular-weight DNA integrity
- Verifying linearized vectors and large inserts before cloning
- Routine molecular biology and teaching laboratories (For Laboratory Use Only. Not for Clinical Use).
Kit Components & Specifications
Catalog No.: DCP-D500-15000B · Size: 1 kit per unit · Base Pair: 500 - 15000 base pairs · Running Buffer: TBE (Tris-Borate-EDTA)
| Product Name | Description | Size | Storage | Shelf Life | Shipping |
|---|---|---|---|---|---|
| DNA Marker | Premixed with loading buffer; sharp reference bands from 500 to 15000 bp for sizing large fragments. Runs ~ 100 gels. | 500 µL | -20°C | 24 months | Blue Ice |
| Loading Buffer [10X] | Adds density so your samples sink into the wells, with tracking dye to monitor migration. Runs ~ 70 gels. | 1 mL | -20°C | 12 months | Blue Ice |
| TBE Buffer [10X] | Concentrated Tris-Borate-EDTA stock. Dilute to 1X for casting agarose gels and as running buffer. Runs ~ 50 small gels. | 1000 mL | RT | 24 months | RT |
Caution
- After thawing is complete, store the DNA Marker and the Loading Buffer [10X] at 4°C. Avoid repeated freezing and thawing.
- Replace the electrophoresis buffer as needed, and use freshly prepared agarose gels to help ensure consistent and reliable electrophoresis results.
- Wear appropriate personal protective equipment, including a lab coat and disposable gloves, while handling the product and performing electrophoresis.
Quality: Manufactured under ISO 13485-certified facilities.
Assay Protocol / Procedures
Ordering Information
Each size range is offered with TAE or TBE running buffer. Choose TAE with the same marker on its own page below.
| Product | Cat No. | Size | Order |
|---|---|---|---|
| DNA Marker (100-700 bp) with TAE & Loading Dye | DCP-D100-700A | 1 kit | Link |
| DNA Marker (100-700 bp) with TBE & Loading Dye | DCP-D100-700B | 1 kit | Link |
| DNA Marker (100-8000 bp) with TAE & Loading Dye | DCP-D100-8000A | 1 kit | Link |
| DNA Marker (100-8000 bp) with TBE & Loading Dye | DCP-D100-8000B | 1 kit | Link |
| DNA Marker (500-15000 bp) with TAE & Loading Dye | DCP-D500-15000A | 1 kit | Link |
| DNA Marker (500-15000 bp) with TBE & Loading Dye | DCP-D500-15000B | 1 kit | Current page |
| All Molecular-Size Markers | – | – | Link |
