Hektoen Enteric Agar Plates/W Kanamycin
Catalog.No: DCP-HEAPT
Features:
Uses: Isolation and differentiation of Salmonella and Shigella from food, water, and environmental samples in food safety and public health labs, and fecal specimens in clinical diagnostics
pH: 7.5 ± 0.2
Size: 100 mm
Concentration: 50µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: green with a yellowish cast; clear to slightly opalescent gel.
Sterile
Shelf Life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Hektoen Enteric Agar (HE) with kanamycin is a modified selective and differential plate medium in which HE formulation is supplemented with an aminoglycoside to further suppress kanamycin-susceptible background flora while retaining Hektoen's ability to differentiate Salmonella and Shigella from other enteric bacteria. The bile salts and dyes of HE's medium help inhibit most Gram-positives and partially or fully inhibit many non-pathogenic coliforms, favoring the growth of enteric pathogens. HE also contains carbohydrates, and fermenting non-target enterics produce acid shifting colony and medium color to yellow to orange/salmon around the colonies. With carbohydrate fermentation salmonella and shigella act as non-fermenters relying on peptone and as a result, they produce alkaline products that make the colony and surrounding medium green to blue-green. Sodium thiosulfate and ferric ammonium citrate help detect H2S by forming black ferrous sulfide, Many Salmonella will have black centers in their colonies.
Kanamycin is an aminoglycoside that binds the 30S ribosomal subunit, causing misreading of mRNA and inhibiting protein synthesis in susceptible bacteria. The combination of HE and kanamycin suppresses kanamycin-susceptible Gram-negative rods and Gram-positive contaminants that would otherwise survive bile and dyes, enriching for kanamycin-resistant Salmonella, Shigella, or engineered enteric strains.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
