Hektoen Enteric Agar Plates/W Amphotericin B
Catalog.No: DCP-HEAPAB
Features:
Uses: Isolation and differentiation of Salmonella and Shigella from food, water, and environmental samples in food safety and public health labs, and fecal specimens in clinical diagnostics
pH: 7.5 ± 0.2
Size: 100 mm
Concentration: 2.5 µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: green with a yellowish cast; clear to slightly opalescent gel.
Sterile
Shelf Life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Hektoen Enteric (HE) agar with amphotericin B is a modified, moderately selective medium in which standard HE formulation is supplemented with an antifungal agent to suppress yeast and mold, allowing clearer recovery and differentiation of enteric bacteria from heavily contaminated specimens. Bile salts inhibit most Gram-positive organisms and suppress many non-pathogenic Gram-negative enterics, the combination of bromothymol blue and acid fuchsin reports pH changes. Fermenting non-target enterics produce acid, turning colonies and surrounding medium yellow to orange/salmon. Salmonella and Shigella behave as non-fermenters with carbohydrates under HE conditions, relying mainly on peptone, yielding alkaline reactions that keep colonies and surrounding medium green to blue-green. Sodium thiosulfate and ferric ammonium citrate yield black ferrous sulfide where H2S is produced. Many Salmonella form green/blue-green colonies with black centers.
Amphotericin B is a polyene antifungal that binds ergosterol in fungal cell membranes, forming pores that increase membrane permeability and cause leakage of intracellular contents. Amphotericin B adds a layer of selectivity by inhibiting most yeasts and filamentous fungi that may otherwise grow from fecal, food, or environmental samples and obscure bacterial colonies. Bacterial colony visibility is improved and maintains HE color reactions, particularly in specimens with high fungal loads.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
