DNA Size Marker, 1 kbp Ladders (1-10 kbp)
DNA Size Marker, 1 kbp Ladders (1-10 kbp), Ready to use
Cat. No. FNK-02-400
Store at -20°C
Size 250µl
DNA ladders 1, 2, 3, 4, 5, 6, 8, 10 kbp (8 bands, see Figure below)
Concentration 0.45 ug/5 ul
Recommended volume of application per lane 5 ul
Solution 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 0.015% bromophenol blue, 2.5% Ficoll-400, 0.017% SDS
Storage For short period (~1 month) at 4℃, for longer period at -20℃
Description
The BioAcademia 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 1 kbp to 10 kbp. The 4 kb band has increased intensity to provide internal orientation. The mass of DNA in each band is provided (assuming a 0.45 μg/ 5 μl load) for approximate quantification in comparably intense samples of similar size.
Figure. Electorphoretic pattern of 1 kbp DNA ladders on 1% agarose gel in TAE buffer. Five ul of the DNA ladder solution was applied.
