Goat anti-mouse IgG, Flamma® 552

Product#: RSA1151
$198.00

Size of product (mg)

  • 0.5 mg
  • 1 mg
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Goat anti-mouse IgG, Flamma® 552

Cat. No. List below

Description

Goat anti-mouse IgG, Flamma® 552 is a fluorescence conjugated secondary antibody that displays excellent optical imaging with low cross reactivity. Anti-mouse secondary antibodies display specificity for mouse IgG and are useful for the detection of specific target. Since multiple secondary antibodies can bind to a single primary antibody, Goat anti-mouse IgG, Flamma® 552 might provide the great sensitivity in signal amplification, visualize low abundant targets and reduce experimental time. Flamma® Fluor dyes feature strong absorption, high fluorescence quantum yield, and high photostability. Flamma® 552 might be excited using 532, 543, 546 or 555 nm laser line and displays excellent optical property. We offer Goat anti-mouse IgG, Flamma® 552 as a suitable fluorescent molecular probe for many biological experiments such as fluorescence microscopy, flow cytometry, microplate assays, protein and nucleic acid blots, in situ hybridization, etc.
 
Specifications
  • Conjugate: Flamma® Fluors 552
  • Species reactivity: Mouse
  • Target: IgG
  • Excitation/Emission Max.(nm): 550/564
  • Host/Isotype: Goat/IgG
  • Type: Secondary antibody
  • Antibody form: Whole antibody
  • Form: Liquid
  • Concentration: 2 mg/mL
  • Storage buffer: PBS (pH 7.4)    
  • Contains: 1.5% BSA, 5 mM sodium azide    
  • Storage conditions: 4 ℃, protect from light
 
Quick link (Cat.#) Series Quick link (Cat.#) Series
RSA1145 Goat anti-mouse IgG, FSD™ 488 RSA1141 Goat anti-mouse IgG, Flamma® 488
RSA1155 Goat anti-mouse IgG, FSD™ 555 RSA1151 Goat anti-mouse IgG, Flamma® 552
RSA1195 Goat anti-mouse IgG, FSD™ 594 RSA1191 Goat anti-mouse IgG, Flamma® 594
RSA1165 Goat anti-mouse IgG, FSD™ 647 RSA1161 Goat anti-mouse IgG, Flamma® 648
RSA1175 Goat anti-mouse IgG, FSD™ 680 RSA1171 Goat anti-mouse IgG, Flamma® 675
RSA1105 Goat anti-mouse IgG, FSD™ 750 RSA1101 Goat anti-mouse IgG, Flamma® 749
RSA1185 Goat anti-mouse IgG, FSD™ 800 RSA1122 Goat anti-mouse IgG, HRP


Background

Fluorescent Protein/Antibody

Fluorescence labeled proteins and antibodies might provide great advantages in the identification of their binding partners or in the observation of biological processes. They allow to visualize cellular environment, to define their function, regulation and interactions. In advanced assays, fluorescent proteins can be utilized in measuring of protein–protein interactions or conformational changes, by measuring intensity changes or FRET energy transfer. An ideal fluorescent label should be small, bright, stable, and without any perturbation to the biological system. Additionally, the fluorescence labeling should be performed at non-active site with the possibility for multiplexing and avoided to the tendency to aggregate.

Organic fluorescent dye-labeled proteins have superior properties over fluorescent protein–conjugated counterparts including, wide spectral range, smaller size, greater photostability, and higher brightness. However, dye–labeled proteins also have some limitations that their sensitivity is low due to lack of the amplification step associated with the secondary antibody, and the protein should be free of contaminants and stabilizing agents. BioActs provides a wide range of fluorophore-conjugated proteins and antibodies for a variety of biological applications. 

Fluorescent Dye conjugated Secondary Antibody

Indirect or secondary immunofluorescence method employs specific interactions between primary and secondary antibody. It is more commonly used than the direct immunofluorescence method due to strong signal amplification and cost-effectiveness. Non-labeled primary antibody in indirect immunofluorescence specifically binds to target molecule, and fluorescence conjugated secondary antibody acts as an antigen of the primary antibody. In addition, the polyclonal nature of secondary antibody features multiplex binding of secondary antibodies per a primary antibody resulting in amplified fluorescence signal. However, since indirect immunofluorescence method utilizes complicated antibody-antibody interaction, the experiment process is complicated and the nonspecific fluorescence signals along with cross reaction might occur. BioActs provides a wide spectrum of fluorescent dye conjugated secondary antibodies directed against IgG from mouse, rabbit, rat, and goat.

In spite of competing approaches such as peptide tagging or mass spectrometry, antibody-based detection is the most broadly used application in analysis of specific protein in complex samples. Fluorescent secondary antibodies are raised against IgG heavy and light chains of the target IgG and minimized cross reactivity by affinity purification and by adsorption against the sera of a number of species. For multiple labeling experiments where cross reactivity is the critical issue, we prepare highly cross-adsorbed goat anti–mouse IgG, goat anti–rabbit IgG, and goat anti–rat IgG fluorescent antibodies. Our antibody probes are conjugated with Flamma® Fluor series, which displays strong fluorescence and photostability, along with ICG and horseradish peroxidase (HRP). Due to their outstanding optical and biological properties, Flamma® Fluor conjugates are superior to most conventional fluorescent secondary antibodies and being optimal molecular probes in many applications. BioActs offers series of fluorescent secondary antibodies as suitable molecular probes for many biological experiments such as fluorescence microscopy, flow cytometry, microplate assays, as protein and nucleic acid blots, in situ hybridization, etc.

 

Citation & Reference

1. Agustina, Lia. Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation. BMC molecular biology 15.1 (2014): 1.

2. Lee, Cheol-Jung. Magnolin inhibits cell migration and invasion by targeting the ERKs/RSK2 signaling pathway. BMC cancer 15.1 (2015): 1.


 

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