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High Sensitivity dsDNA Quantification Kit, 100 assays - for Accuris™ Smart-Q™ Fluorometer

Product#: BM-F1000-HS-100

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High Sensitivity dsDNA Quantification Kit, 100 assays - for Accuris™ Smart-Q™ Fluorometer

Cat. No.: BM-F1000-HS-100

Description

The Accuris dsDNA HS Quantitation Kit provides a simple, sensitive and accurate quantitation for dsDNA. The kit includes concentrated assay reagent, dilution buffer, and prediluted dsDNA standards. The assay kit is highly sensitive and selective for dsDNA due to fluorescence dye high quantum yield and large molar extinction coefficient.

The kit is highly reliable in detecting dsDNA with initial sample concentrations from 0.005 ng/μL to 120 ng/μL ranging from 0.1 to 120 ng. The kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The reagent is simply diluted using the buffer provided, added your sample (any volume between 1 μL and 20 μL is acceptable), and then read the concentration using a fluorometer.

Kit Components & Storage Requirements

Material	Storage	Amount	Concentration
Accuris HS dsDNA Reagent (Component 1)	4 ℃ Protect from light	100 μL	200 X in DMSO
Accuris HS dsDNA Buffer (Component 2)	4 ℃	25 mL	1 X
Accuris HS dsDNA Standard #1 (Component 3)	4 ℃	200 μL	0 ng/μL
Accuris HS dsDNA Standard #2 (Component 4)	4 ℃	200 μL	10 ng/μL

Package contents and reordering

Accuris High Sensitivity dsDNA Quantification Kit, 100 assays - Catalog number NS1020-HS-100

General Protocol

Preparation

1. Warm up the dsDNA HS Quantitation Kit to room temperature. Check the dsDNA HS reagent for any precipitation. If precipitation is seen, warm up the vial in a water bath and vortex until dissolved.

2. Prepare the working solution by diluting the dsDNA HS reagent 1:200 in 1× dsDNA HS buffer. Use a clean plastic tube each time to make working solution. For example, to measure 8 samples in duplicate, add 10 μL of dsDNA HS reagent to 1990 μL of dsDNA HS Buffer. Mix well and use IMMEDIATELY. Once mixed into the working solution, samples must be measured within 3 hours to prevent degradation of fluorescence intensity

Standard Curve

1. Add 190 μL of the working solution to each assay tube. (Note: Use only thinwall, clear 0.5 mL PCR tubes for fluorescence analysis.

2. Add 10 μL of dsDNA standard #1 (Component 3), dsDNA standard #2 (Component 4) into separated tubes, and mix by vortexing (5-10 seconds), and incubate all tubes at room temperature for 3 minutes in the dark. Note: When mixing dsDNA standard and working solution, please disperse the bubbles before analysis.

3. Measure the fluorescence using the calibration program of standard curve. Click dsDNA in the Home interface, select “dsDNA: High Sensitivity” and press the button. Select “Calibration” from the pop-up box. Standard 1 should be set by default as having 0 concentration. Insert standard 1 into the fluorometer port and click “Read standards” to perform the measurement. Once finished, proceed to set up and measure standard 2. After calibration, samples are ready to be measured.

Measuring Samples

1. Add the sample (any volume between 1 μL and 20 μL is acceptable) and the working solution, and the final volume in each tube should be 200 μL.

2. Mix by vortexing (5-10 seconds). Incubate all tubes at room temperature for 3 minutes in the dark. Note: When mixing dsDNA standard and working solution, please disperse the bubbles before analysis.

3. Place the samples into the fluorometer for measurement

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Smart-Q Fluorometer, for quantification of DNA, RNA and protein samples

Sensitive quantitation down to 0.1pg/ul ds DNA
Large color touch screen for programming and operation
Two models for single or 8-sample processing
Built-in reagent calculators for sample preparation
Compact and portable (11 x 6 inch footprint)

Smart-Q Specifications

Model	Smart-Q™ 100 Fluorometer	Smart-Q™ 800 Fluorometer	Smart-Q™ Mini 115V	Smart-Q™ Mini 230V
Display	7.0-inch, color touch screen	7.0-inch, color touch screen	No external display (instrument operation via iOS/Android phone or tablet)	No external display (instrument operation via iOS/Android phone or tablet)
Dynamic Range	5 orders of magnitude	4 orders of magnitude	5 orders of magnitude	5 orders of magnitude
Light Source	Blue and Red LED	Blue and Red LED	Blue and Red LED	Blue and Red LED
Excitation Wavelengths	Blue: 460 ± 20nm and Red: 625 ± 20nm	Blue: 460 ± 20nm and Red: 625 ± 20nm	Blue: 470 ± 15 nm Red: 620 ± 20 nm	Blue: 470 ± 15 nm Red: 620 ± 20 nm
Emission Wavelengths	Green: 525 – 570nm and Red: 670 – 725nm	Green: 525 – 570nm and Red: 670 – 725nm	Green: 525 – 570nm and Red: 670 – 725nm	Green: 525 – 570nm and Red: 670 – 725nm
Stability	≤ 1.5%	≤ 1.5%	≤1.5%	≤ 1.5%
Detector	Photodiodes (300-1000nm)	Photodiodes (300-1000nm)	Photodiodes (320-1100nm)	Photodiodes (320-1100nm)
Measurement Speed	≤ 5 seconds	≤ 6 seconds	≤ 5 seconds	≤ 5 seconds
Consumable type	0.5mL assay tubes	0.2mL assay tubes (single and 8-tube strip)	0.5 mL optically-clear quantification tubes	0.5 mL optically-clear quantification tubes
Input Voltage	24 VDC 2A	24 VDC 2A	115V	230V
Dimensions (WxDxH)	16.1 x 28.7 x 6.1cm / 6.34 x 11.30 x 2.40in.	16.1 x 28.7 x 7.6 cm / 6.3 x 11.3 x 3.0 in.	3.27 x 3.27 x 3.62 in. / 8.13 x 8.13 x 3.19 cm	3.27 x 3.27 x 3.62 in. / 8.13 x 8.13 x 3.19 cm
Weight	1.6kg / 3.5lbs	2.0kg / 4.4lbs	0.5kg / 1.1lbs	0.5kg / 1.1lbs
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Description

	The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving: Next Generation Sequencing PCR Transfection Western blotting Immunoassays The Smart-Q Fluorometers are engineered to achieve optimal performance with Accuris’ Fluorescent DNA Quantification Kits and assay tubes; however, they also maintain compatibility with a range of kits offered by various other suppliers. Intuitive Usability Each instrument is equipped with a 7-inch color touchscreen, featuring an intuitive, icon-driven user interface. Preprogrammed protocols allow for quick & effortless assay set-up. Results are quickly calculated and displayed in less than 6 seconds, and saved to the instrument’s internal memory. All data can be also be exported easily to a PC using a USB flash drive. Accurate Quantification The Smart-Q Fluorometers, along with their accompanying assay kits, are engineered for the precise measurement of fluorescent dye signals that are bound to targeted biological molecules. These specially formulated dyes exhibit selective affinity towards DNA, RNA, or proteins, and generate fluorescent signals exclusively upon binding to their respective targets. Both Smart-Q models include Blue and Red channels that allow for various fluorescent dyes to be used in nucleic acid and protein measurements. The Smart-Q Fluorometers incorporate advanced curve-fitting algorithms to establish calibration curves from standard samples of known concentrations. The concentration of an unknown sample, whether it be DNA, RNA, or protein, is determined by correlating its Relative Fluorescence Units (RFUs) with those of the standards employed during calibration. The measurement sensitivity for each type of assay is distinct and defined by its detection limits. Options for Throughput The Smart-Q portfolio includes two distinct models, each designed to meet specific laboratory requirements: The Smart-Q 100 allows for single-sample processing in 0.5mL assay tubes (NS1020-T). This renders the Smart-Q 100 ideal for laboratories processing fewer samples. The Smart-Q 800 caters to high-throughput workflows and allows for up to eight samples to be evaluated at once using individual 0.2mL tubes or 8 x 0.2mL strips tubes (Order no. F1001-T and F1001-T-8, ). The use of a standard 8-channel pipettor can streamline both the reagent preparation and sample transfer, making the Smart-Q 800 an efficient solution for workflows requiring parallel processing of multiple samples. Accuris DNA Quantification Kits Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA using an instrument such as the SmartDrop XF, Qubit™or Accuris™ Fluorometer. The reagents are highly selective for double-stranded DNA (dsDNA), and not for single-stranded DNA (ssDNA), RNA, protein, or nucleotides. Included with each kit are a concentrated assay reagent, dilution buffer, and ready-to-use DNA standards. The assay reagent is diluted using the buffer provided, the sample is added (1 µL to 20 µL of sample is needed), and the concentration can be determined using an appropriate fluorometer instrument. High Sensitivity dsDNA Assay Kit The Accuris High Sensitivity kit provides an accurate method for the quantitation of dsDNA samples when sensitivity is required. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.005 to 120 ng/µL, providing a detection range of 0.1−120 ng. 0.1 to 100 ng/ul, providing detection of 0.1 to 100 ng Broad Range dsDNA Assay Kit The Accuris Broad Range kit provides an accurate method for quantitation of dsDNA samples for a broad range quantity of DNA. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng. ssDNA Quantification Kit The Accuris ssDNA Kit provides a simple and accurate method for quantifying single-stranded DNA (ssDNA) or oligonucleotides. The assay kit is accurate at detecting ssDNA between 0.05-200 ng/µL. While the kit is not selective for ssDNA over double-stranded DNA (dsDNA) or RNA, it does not detect contaminating proteins or nucleotides. Additionally, the kit demonstrates insensitivity to low concentrations of common contaminants such as proteins, salts, solvents, and detergents RNA High Sensitivity Quantification Kit The Accuris RNA High Sensitivity Quantification Kit offers a reliable and accurate method for measuring RNA concentration. This assay is highly selective for RNA over double-stranded and single-stranded DNA and can detect RNA concentrations ranging from 0.1 ng/μL to 100 ng/μL RNA Broad Range Quantification Kit The Accuris RNA Broad Range Quantification Kit enables precise and reliable RNA quantification. This assay exhibits high specificity for RNA over single stranded and double-stranded DNA, allowing for the detection of RNA concentrations ranging from 1 ng/μL to 1000 ng/μL. This assay demonstrates robustness against common contaminants, including proteins, free nucleotides, salts, solvents, and detergents


Smart-Q 100 for single sample processing

Smart-Q 800 for up to 8-sample processing

Accuris DNA Quantification Kits

Ordering Information

Smart-Q™	Cat.No.	Products	Qty ( Pack )	Order
	BM-F1001	Smart-Q 100 Fluorometer, single sample	1
	BM-F1008	Smart-Q 800 Fluorometer, 8-channel1	1
	BM-F0100	Smart-Q Mini Fluorometer, 115V	1
	BM-F0100-E	Smart-Q Mini Fluorometer, 230V	1
	BM-F0100-TAB	Android tablet preloaded with Smart-Q Mini app	1
	BM-F1000-HS-100	High Sensitivity dsDNA Quantification Kit, 100 assays	1 Kit	Current page
	BM-F1000-HS-500	High Sensitivity dsDNA Quantification Kit, 500 assays	1 Kit
	BM-F1000-HS1-100	1X High Sensitivity dsDNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-HS1-500	1X High Sensitivity dsDNA Quantification Kit, 500 assays	1 Kit
	BM-F1000-BR-100	Broad Range dsDNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-BR-500	Broad Range dsDNA Quantification Kit, 500 assays	1 Kit
	BM-F1000-BR1-100	1X Broad Range dsDNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-BR1-500	1X Broad Range dsDNA Quantification Kit, 500 assays	1 Kit
	BM-F1000-SD-100	ssDNA Quantification Kit, 100 Assays	1 Kit
	BM-F1000-SD-500	ssDNA Quantification Kit, 500 Assays	1 Kit
	BM-F1000-RHS-100	High Sensitivity RNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-RHS-500	High Sensitivity RNA Quantification Kit, 500 assays	1 Kit
	BM-F1000-RBR-100	Broad Range RNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-RBR-500	Broad Range RNA Quantification Kit, 500 assays	1 Kit
	BM-F1001-T	0.5mL Fluorescence Detection Tubes, pack of 250	250
	BM-F1008-T	0.2ml Smart-Q™ 800 Assay Tubes, pack of 1000	1000
	BM-F1008-T-8	0.2ml Smart-Q™ 800 Assay Tube Strips, pack of 120	120