Levine EMB Agar Plates
Catalog.No: DCP-LEMBAP
Features:
pH: 7.1 ± 0.2
Uses: Isolation and differentiation of coliforms and fecal coliforms from clinical, food, water, and environmental samples.
Size: 100 mm
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: clear, red-brown to dark red, slightly amber when held to light. When tetracycline or other antibiotics are added for yeast isolation (e.g., Candida), the medium can appear more blue-tinged due to dye interactions.
Sterile
Shelf life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish
Description:
Levine EMB (Eosin Methylene Blue) agar is a low selective differential medium used mainly for coliform and enteric Gram-negative isolation and differentiation, especially fecal coliforms such as escherichia coli. The dyes eosin Y and methylene blue interact with the cell walls and membranes of many Gram-positive bacteria reducing their ability to grow or form normal colonies, so Gram-negative rods are favored. Levine EMB contains lactose as the fermentable carbohydrate, bacteria that are able to ferment lactose produce organic acids, lowering the pH of the medium around their colonies. Moderate/slow lactose fermenters produce less acid, often not enough for intense sheen, colonies appear pink to purple or brownish, frequently mucoid with darker centers and paler edges. Non-lactose fermenters do not acidify the medium from lactose, leaving the pH either neutral or slightly alkaline.
*Due to Levine EMB being dye-based, most antibiotics may yield different results from the intended results of standard Levine EMB. For antibiotics please contact us for customization options
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
