Goat anti-rabbit IgG, FSD™ 647

Product#: RSA1265
$1.00

Size of product (mg)

  • Trial(0.2 mg)
  • 0.5 mg
  • 1 mg
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Goat anti-rabbit IgG, FSD™ 647

Cat. No. List below

Description

Goat anti-rabbit IgG, FSD™ 647 is a fluorescence conjugated secondary antibody that displays excellent optical imaging with low cross reactivity. Anti-rabbit secondary antibodies display specificity for rabbit IgG and are useful for the detection of specific target. Since multiple secondary antibodies can bind to a single primary antibody, Goat anti-rabbit IgG, FSD™ 647 might provide the great sensitivity in signal amplification, visualize low abundant targets and reduce experimental time. FSD Fluor™ is a new generation of dye series with superb fluorescence intensity and high quantum yield comparing to traditional dyes. FSD™ 647 might be excited using 593 or 633 nm laser line and displays excellent optical property. We offer Goat anti-rabbit IgG, FSD™ 647 as a suitable fluorescent molecular probe for many biological experiments such as fluorescence microscopy, flow cytometry, microplate assays, protein and nucleic acid blots, in situ hybridization, etc.
 
Specifications
  • Conjugate: FSD™  647
  • Species reactivity: Rabbit
  • Tar get: IgG
  • Excitation/Emission Max.(nm): 651/667
  • Host/Isotype: Goat/IgG
  • Type: Secondary antibody
  • Antibody form: Whole antibody
  • Form: Liquid
  • Concentration: 2 mg/mL
  • Storage buffer: PBS (pH 7.4)    
  • Contains: 1.5% BSA, 5 mM sodium azide    
  • Storage conditions: 4 ℃, protect from light
 
Quick link (Cat.#) Series Quick link (Cat.#) Series
RSA1245 Goat anti-rabbit IgG, FSD™ 488 RSA1241 Goat anti-rabbit IgG, Flamma® 488
RSA1255 Goat anti-rabbit IgG, FSD™ 555 RSA1251 Goat anti-rabbit IgG, Flamma® 552
RSA1295 Goat anti-rabbit IgG, FSD™ 594 RSA1291 Goat anti-rabbit IgG, Flamma® 594
RSA1265 Goat anti-rabbit IgG, FSD™ 647 RSA1261 Goat anti-rabbit IgG, Flamma® 648
RSA1275 Goat anti-rabbit IgG, FSD™ 680 RSA1271 Goat anti-rabbit IgG, Flamma® 675
RSA1205 Goat anti-rabbit IgG, FSD™ 750 RSA1201 Goat anti-rabbit IgG, Flamma® 749
RSA1285 Goat anti-rabbit IgG, FSD™ 800 RSA1221 Goat anti-rabbit IgG, HRP


Background

Fluorescent Protein/Antibody

Fluorescence labeled proteins and antibodies might provide great advantages in the identification of their binding partners or in the observation of biological processes. They allow to visualize cellular environment, to define their function, regulation and interactions. In advanced assays, fluorescent proteins can be utilized in measuring of protein–protein interactions or conformational changes, by measuring intensity changes or FRET energy transfer. An ideal fluorescent label should be small, bright, stable, and without any perturbation to the biological system. Additionally, the fluorescence labeling should be performed at non-active site with the possibility for multiplexing and avoided to the tendency to aggregate.

Organic fluorescent dye-labeled proteins have superior properties over fluorescent protein–conjugated counterparts including, wide spectral range, smaller size, greater photostability, and higher brightness. However, dye–labeled proteins also have some limitations that their sensitivity is low due to lack of the amplification step associated with the secondary antibody, and the protein should be free of contaminants and stabilizing agents. BioActs provides a wide range of fluorophore-conjugated proteins and antibodies for a variety of biological applications. 

Fluorescent Dye conjugated Secondary Antibody

Indirect or secondary immunofluorescence method employs specific interactions between primary and secondary antibody. It is more commonly used than the direct immunofluorescence method due to strong signal amplification and cost-effectiveness. Non-labeled primary antibody in indirect immunofluorescence specifically binds to target molecule, and fluorescence conjugated secondary antibody acts as an antigen of the primary antibody. In addition, the polyclonal nature of secondary antibody features multiplex binding of secondary antibodies per a primary antibody resulting in amplified fluorescence signal. However, since indirect immunofluorescence method utilizes complicated antibody-antibody interaction, the experiment process is complicated and the nonspecific fluorescence signals along with cross reaction might occur. BioActs provides a wide spectrum of fluorescent dye conjugated secondary antibodies directed against IgG from mouse, rabbit, rat, and goat.

In spite of competing approaches such as peptide tagging or mass spectrometry, antibody-based detection is the most broadly used application in analysis of specific protein in complex samples. Fluorescent secondary antibodies are raised against IgG heavy and light chains of the target IgG and minimized cross reactivity by affinity purification and by adsorption against the sera of a number of species. For multiple labeling experiments where cross reactivity is the critical issue, we prepare highly cross-adsorbed goat anti–mouse IgG, goat anti–rabbit IgG, and goat anti–rat IgG fluorescent antibodies. Our antibody probes are conjugated with Flamma® Fluor series, which displays strong fluorescence and photostability, along with ICG and horseradish peroxidase (HRP). Due to their outstanding optical and biological properties, Flamma® Fluor conjugates are superior to most conventional fluorescent secondary antibodies and being optimal molecular probes in many applications. BioActs offers series of fluorescent secondary antibodies as suitable molecular probes for many biological experiments such as fluorescence microscopy, flow cytometry, microplate assays, as protein and nucleic acid blots, in situ hybridization, etc.

 

Citation & Reference

1. Agustina, Lia. Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation. BMC molecular biology 15.1 (2014): 1.

2. Lee, Cheol-Jung. Magnolin inhibits cell migration and invasion by targeting the ERKs/RSK2 signaling pathway. BMC cancer 15.1 (2015): 1.

3. Promoter- and RNA polymerase II-dependent hsp-16 gene association with nuclear pores in Caenorhabditis elegans. Rohner S,Kalck V,Wang X,Ikegami K,Lieb JD,Gasser SM,Meister P The Journal of cell biology (200:589) 

4. BRAG2/GEP100/IQSec1 interacts with clathrin and regulates α5β1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5). Moravec R,Conger KK,D'Souza R,Allison AB,Casanova JE The Journal of biological chemistry (287:31138)


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