Flamma® 675 Sulfo-NHS ester
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 675 Sulfo-NHS ester
Cat. No. List below
Description
Flamma® Fluors 675 Sulfo-NHS Ester is a state-of-the-art near-infrared (NIR) fluorescent dye engineered for exceptional performance in bioimaging applications. This reactive dye, derived from benzindocyanine structure, offers superior water solubility and efficient biomolecule labeling, making it an ideal choice for researchers seeking high-quality fluorescence signals in biological studies.
Key Features:
- Enhanced Water Solubility: Sulfo-NHS ester group enables labeling without organic co-solvents
- Efficient Conjugation: Readily reacts with amine-modified biomolecules
- Spectral Properties: Ex/Em maxima at 675/691 nm, comparable to popular dyes like Alexa 680 and Cy5.5
- NIR Emission: Ideal for deep tissue imaging with minimal background interference
- Versatile Applications: Suitable for labeling antibodies, peptides, proteins, ligands, and amplification substrates
- High Sensitivity: Capable of detecting low-abundance biomolecules in fixed cells
Flamma® Fluors 675 Sulfo-NHS Ester is particularly well-suited for fixed cell imaging which has high sensitivity for detecting low-abundance biomolecules in fixed cellular samples, protein trafficking studies, which track labeled proteins through cellular compartments with minimal background, multiplexing experiments, which combine with other fluorophores for multi-color imaging studies, and In Vitro assays, which is ideal for various biochemical and cellular assays requiring sensitive fluorescence detection.
Flamma® Fluors 675 Sulfo-NHS Ester represents a significant advancement in NIR fluorescent dyes for bioimaging. Its unique combination of water solubility, efficient conjugation, and superior spectral properties makes it an excellent choice for researchers demanding high-quality, reliable results in their fluorescence-based experiments. Whether you're conducting in vitro imaging, protein labeling, or exploring complex biological systems, this dye offers the performance and versatility needed to push the boundaries of your research in the near-infrared spectrum.
- Fluorophore: Flamma® Fluors 675
- Reactive group: Sulfo-NHS ester
- Excitation/Emission Max.(nm): 675/691
- Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
- Extinction coefficient: ≥ 172,000 cm -1M-1
- CF280: 0.09
- Appearance: Blue Solid
- Molecular Weight: 1122.24 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWSN1001 | Flamma® 496 Sulfo-NHS ester | 496 | 520 | Alexa488, FITC, Cy2 |
| PWSN1122 | Flamma® 552 Sulfo-NHS ester | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWSN1415 | Flamma® 581 Sulfo-NHS ester | 581 | 596 | Alexa594, DyLight594 |
| PWSN1215 | Flamma® 648 Sulfo-NHS ester | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWSN1515 | Flamma® 675Sulfo-NHS ester | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWSN1301 | Flamma® 749 Sulfo-NHS ester | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWSN1603 | Flamma® 774 Sulfo-NHS ester | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
- Superior Water Solubility: The Sulfo-NHS ester group eliminates the need for organic co-solvents, simplifying labeling protocols and reducing potential interference with biomolecules.
- Efficient Coupling: Readily reacts with primary amines, forming stable amide bonds between the dye and biomolecules, including ε-amino groups of lysine and amine termini of nucleotides.
- Spectral Compatibility: Similar spectral properties to widely used dyes like Alexa 680, Cy5.5, IRDye 680LT, and DyLight 680, allowing for seamless integration into existing imaging setups.
- Deep Tissue Imaging: NIR emission enables imaging of deeper tissues with reduced autofluorescence and improved signal-to-noise ratio.
- Versatile Excitation: Can be excited using a 633 nm laser line, compatible with many common fluorescence microscopy systems.
Flamma® Fluors NHS ester / Sulfo-NHS ester
Succinimidyl (NHS) esters are reliable activating moiety for amine labeling due to the formation of the strong amide bond. NHS esters are generally stable when storing in anhydrous and the dark condition at -20. NHS ester dyes display good reactivity toward aliphatic amines yet low reactivity with aromatic amines, alcohols, phenols and histidine. The basicity of an amine is an important factor for its reactivity: virtually all proteins have lysine residues, and most of them have a free N-terminus amine. The reactivity of ε-amino group at lysine is pH dependent that the optimal pH for labeling lysine residue is 8.5~9.5. However, the primary amine at N-terminus usually has a pKa value of ~7, thus it can be selectively conjugated by running the reaction in neutral pH. Due to the solubility issue, Flamma® Fluors NHS esters are required to be dissolved in an organic solvent such as DMSO or DMF and added to aqueous solution. To overcome this limitation, BioActs offers water soluble Flamma® Fluors Sulfo-NHS ester dyes, which can eliminate the usage of organic solvents in the conjugation reaction. NHS and Sulfo-NHS ester dyes are unstable under humid condition, thus they should be in a lyophilized form or a solution in the anhydrous organic solvent such as DMF or DMSO and stored at -20 ℃.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse
Citation & Reference
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