FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Phenol Red: 1X Liquid

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Phenol Red: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 using Ultrapure Type 1 water (18.2 MΩ·cm). Formulated without glucose and without phenol red, with 25 mM HEPES and sodium bicarbonate buffering, for precise control of carbon source and pH in metabolic research.

• Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size, with mycoplasma-retentive filtration at each 0.1 µm stage
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> Bacterial Endotoxins Test)
• 25 mM HEPES plus 44 mM sodium bicarbonate buffering; formulated without glucose and without phenol red for precise metabolic control
• 584 mg/L L-glutamine and 110 mg/L sodium pyruvate supply an alternative carbon source in the absence of glucose
• Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis
• Ultrapure Type 1 water (18.2 MΩ·cm) supports low trace-metal and organic-carbon background for sensitive biosensor applications
• 4× BME amino acid & vitamin concentrations for robust long-duration MPS perfusion
• Custom pH, glucose, HEPES, salts & nutrients — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — capturing what conventional 0.22 µm filtration misses.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final filter and preserves microchannel chip geometries.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains fine particulates and microaggregates not retained by standard 0.22 µm filtration.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge for full-train redundancy.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill & finish in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Phenol Red: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

For fully automated bioreactor and liquid-handling robotics platforms, Diagnocine also offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation, built on a six-stage cascade (0.1 µm ×2 → 0.04 µm ×2 → 0.02 µm → 0.01 µm).

• Total Particulate Exclusion — sub-10 nm cut-off for long-duration closed-loop systems
• Valve & Sensor Protection — minimizes fouling of automated dosing valves and inline sensors
• Extended Perfusion Stability — supports multi-week unattended perfusion runs

Inquiry Required: The MPS Grade 0.01 µm variant is produced to order. Contact support@diagnocine.com to request this formulation.

Full technical specification

Every lot of FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Phenol Red: 1X Liquid is released against comprehensive multi-parameter QC specifications.

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 32 components across 4 categories, displayed in 3 tabs above. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Manufacturing & compliance

Every batch subjected to multi-parameter lot-release testing before distribution.

How DCP-DMEMH-GR1X compares

Critical advantages in filtration, mycoplasma-retentive filtration, water quality, and QC depth for MPS and microfluidic applications.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Phenol Red: 1X Liquid (DCP-DMEMH-GR1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x