- Home
- FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), 50mM HEPES w/o Glucose, Sodium Pyruvate, Phenol Red: 2X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), 50mM HEPES w/o Glucose, Sodium Pyruvate, Phenol Red: 2X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 50mM HEPES w/o Glucose, Sodium Pyruvate, Sodium Bicarbonate: 2X Liquid
Double-strength (2X), glucose-free DMEM with 50 mM HEPES (25 mM at 1X) — you set the glucose or carbon source — for glucose-deprivation, dose-response, glucose-uptake and other metabolic studies, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a 50 mM HEPES buffer, bicarbonate-free, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.
- 2X concentrate: mix with an equal volume of a compatible solution or matrix for a final 1X medium
- Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
- 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
- 50 mM HEPES (25 mM at 1X) for CO₂-independent pH stability during carbon-source additions and handling
- Endotoxin < 0.05 EU/mL (USP <85> BET)
- Formulation: [+] L-Glutamine, [+] Phenol Red, [+] 50mM HEPES; [−] Glucose, [−] Sodium Pyruvate, [−] Sodium Bicarbonate
- Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
- Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
- D-GlucoseNot included
- L-Glutamine1,168 mg/L
- Sodium PyruvateNot included
- Buffer50 mM HEPES (no NaHCO₃)
- Phenol Red31.8 mg/L
- AppearanceRed to Orange
- pH7.4 (± 0.04)
- Endotoxin< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Format2X liquid, 500 mL
Engineered where standard media fails
Conventional DMEM is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and hydrogels, and add noise to optical readouts.[11,12] FluxMPS™ DMEM keeps Dulbecco's formulation and removes that failure mode.
Microchannel-safe purity
A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and matrices.
Total metabolic control
No glucose, so you set the carbon source exactly; 1,168 mg/L L-glutamine and no sodium pyruvate in the 2X (half that at 1X).
Ultrapure-grade water
Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.
Low background for imaging
Particle-depleted medium reduces scatter and debris artifacts in colony counting, confocal imaging and on-chip sensing.
Matrix-ready 2X strength
31 components at double strength, so agar, collagen, hydrogels or treatment solutions can be added 1:1 without diluting the medium below 1X.
Customization on demand
pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of DCP-DMEMH-GPB2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X DMEM polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.
-
01
0.1 µmPre-filtration I
Removes large particulates and aggregates and extends the life of the downstream filters.
-
02
0.04 µmPre-filtration II
Retains fine particulates, bacteria and mycoplasma.
-
03
0.1 µmSterile-filtration I
Second-pass redundancy that catches anything shed or bypassed upstream.
-
04
0.04 µmSterile-filtration II — Final Polish
Final nano-polish immediately before ISO Class 5 aseptic fill.
Performance vs. conventional media
By particulate count, FluxMPS™ DMEM is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.
© Diagnocine® — DCP-DMEMH-GPB2X
Where FluxMPS™ DMEM fits
DMEM No Glucose is a glucose-free basal medium designed for studies requiring precise control of glucose or other carbon sources.[1,2] It allows researchers to create customized glucose concentrations and investigate cellular responses to glucose deprivation, metabolic stress, altered energy availability, and experimental carbohydrates.
This medium is commonly used in cancer metabolism, diabetes and insulin-signaling research, glucose-uptake studies, autophagy and AMPK signaling, ischemia or hypoxia models, and other metabolic research. Because it contains no glucose, supplementation may be required depending on the cell type and experimental design.
DMEM No Glucose 2X is a double-strength, glucose-free formulation designed to be combined with an equal volume of another compatible solution while maintaining the remaining DMEM components at their intended final 1X concentrations. The formulation remains glucose-free unless glucose or another carbohydrate is added separately. Researchers may use the 2X formulation to incorporate glucose solutions, alternative carbon sources, treatment solutions, hydrogels, agarose, methylcellulose, cell suspensions, or other high-volume components without diluting the basal nutrients.[4,5]
It is useful for:
- In vitro glucose-deprivation and metabolic-stress studies.
- Custom glucose dose-response experiments.
- Cell-based glucose-uptake and insulin-signaling research.
- In vitro cancer-metabolism and diabetes-related research.
- Autophagy and AMPK-signaling pathway studies.
- Laboratory models of ischemia, hypoxia, and nutrient starvation.
- Evaluation of galactose and other alternative carbon sources.[3]
- Semisolid, hydrogel, 3D cell-culture, and organ-on-a-chip applications.
Automated Bioreactors & Robotics
For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.
- Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
- Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
- Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.
Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.
Glucose Deprivation & Dose-Response
Run glucose-free starvation arms and custom glucose titrations from one base medium by adding glucose to the mixing solution.
Cancer Metabolism & Galactose Switch
Replace glucose with galactose to force oxidative phosphorylation and reveal mitochondrial liabilities.[3]
Glucose Uptake & Insulin Signaling
A glucose-free starting point for glucose-uptake assays and insulin-signaling studies.
Autophagy, AMPK & Ischemia Models
Model nutrient starvation, energy stress and in vitro ischemia or hypoxia, alone or with oxygen control.
Organ-on-a-Chip & MPS
Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms.[8,9,10]
Microscopy & Optical Sensing
Fewer particles means cleaner fields for colony counting, confocal imaging and on-chip biosensors.
Specifications & quality control
Release specifications for DCP-DMEMH-GPB2X. Lot-specific values are reported on the Certificate of Analysis.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Phenol Red, [+] 50mM HEPES; [−] Glucose, [−] Sodium Pyruvate, [−] Sodium Bicarbonate |
| Concentration | 2X (dilute 1:1 to 1X) |
| Appearance | Red to Orange |
| pH | 7.4 (± 0.04) USP <791> |
| Osmolality | Measured per lot; reported on CoA USP <785> |
| D-Glucose | Not included (add to your target concentration) |
| L-Glutamine | 1,168 mg/L |
| Sodium Pyruvate | Not included |
| Sodium Bicarbonate | Not included |
| Phenol Red | 31.8 mg/L |
| HEPES | 11,916 mg/L (50 mM; 25 mM at 1X) |
| Parameter | Specification |
|---|---|
| Endotoxin | < 0.05 EU/mL USP <85> BET |
| Sterility | No bacterial or fungal growth after 14 days of incubation USP <71> |
| Mycoplasma | 0.04 µm barrier filtration USP <63> equiv. |
| Particulate ≥10 µm | Meets limits USP <788> M2 |
| Particulate ≥25 µm | Meets limits USP <788> M2 |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) ISO |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8 °C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months; use before the expiry date on the product label |
| Shipping condition | Ice Pack |
| CO₂ requirement | Not required for HEPES buffering (50 mM in the 2X, 25 mM at 1X); add sodium bicarbonate if the final medium will be held in a CO₂ incubator |
| Parameter | Specification |
|---|---|
| Raw material grade | High-purity, cell-culture-tested components |
| Traceability | Lot-traceable; CoA per lot |
| Manufacturing QMS | ISO 13485:2016 ISO |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
DCP-DMEMH-GPB2X is glucose-free 2X DMEM with L-glutamine, Phenol Red and 50 mM HEPES. It does not contain glucose, sodium pyruvate nor sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 31 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 dilution.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 530.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.200 |
| Magnesium sulphate anhydrous | 7487-88-9 | 195.440 |
| Potassium chloride | 7447-40-7 | 800.000 |
| Sodium chloride | 7647-14-5 | 12800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 218.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 60.000 |
| L-Arginine hydrochloride | 1119-34-2 | 168.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 125.140 |
| L-Glutamine | 56-85-9 | 1168.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 84.000 |
| L-Isoleucine | 73-32-5 | 210.000 |
| L-Leucine | 61-90-5 | 210.000 |
| L-Lysine hydrochloride | 657-27-2 | 292.000 |
| L-Methionine | 63-68-3 | 60.000 |
| L-Phenylalanine | 63-91-2 | 132.000 |
| L-Serine | 56-45-1 | 84.000 |
| L-Threonine | 72-19-5 | 190.00 |
| L-Tryptophan | 73-22-3 | 32.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 207.580 |
| L-Valine | 72-18-4 | 188.00 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 8.000 |
| D-Ca-Pantothenate | 137-08-6 | 8.000 |
| Folic acid | 59-30-3 | 8.000 |
| Nicotinamide | 98-92-0 | 8.000 |
| Pyridoxine hydrochloride | 58-56-0 | 8.000 |
| Riboflavin | 83-88-5 | 0.800 |
| Thiamine hydrochloride | 67-03-8 | 8.000 |
| i-Inositol | 87-89-8 | 14.400 |
| OTHERS | ||
| Phenol Red | 34487-61-1 | 31.800 |
| HEPES | 7365-45-9 | 11916.000 |
Manufacturing & compliance
Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.
ISO 13485:2016 QMS
Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).
Ultrapure Type 1 Water
18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.
ISO Class 5 Fill & Finish
Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.
Micro-Batch Precision
Small, tightly controlled batches for consistent composition and pH from lot to lot.
Endotoxin — USP <85> BET
Released at < 0.05 EU/mL.
Particulate — USP <788> Method 2
Subvisible particles ≥10 µm and ≥25 µm within compendial limits.
Osmolality — USP <785>
Measured per lot and reported on the CoA.
Documentation / CoA
Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.
How DCP-DMEMH-GPB2X compares
Same DMEM chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.
| Parameter | DCP-DMEMH-GPB2X (FluxMPS™) | Conventional DMEM no glucose + HEPES, 1X (0.22 µm filtered) | Conventional 2X DMEM (0.22 µm filtered) |
|---|---|---|---|
| Formulation | 2X glucose-free DMEM + 50 mM HEPES; free of Glucose, Sodium Pyruvate and sodium bicarbonate | 1X DMEM, ready to use | Double-strength, for semisolid culture |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin specification | < 0.05 EU/mL | Often not specified at this level | Often not specified at this level |
| USP <788> particulate compliance | check_circle | cancel | cancel |
| Ultrapure Type 1 water (18.2 MΩ·cm) | check_circle | Varies | Varies |
| ISO 13485:2016 manufacturing | check_circle | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about using DCP-DMEMH-GPB2X for semisolid, 3D, microfluidic and conventional culture.
Supporting literature
Peer-reviewed background on DMEM, HEPES buffering, glucose/galactose substitution, semisolid and collagen-gel culture, Phenol Red, organ-on-a-chip culture and mycoplasma control.
- Dulbecco R, Freeman G. Plaque production by the polyoma virus. Virology. 1959;8(3):396–397. doi:10.1016/0042-6822(59)90043-1
- Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
- Marroquin LD, Hynes J, Dykens JA, Jamieson JD, Will Y. Circumventing the Crabtree effect: replacing media glucose with galactose increases susceptibility of HepG2 cells to mitochondrial toxicants. Toxicol Sci. 2007;97(2):539–547. doi:10.1093/toxsci/kfm052
- Borowicz S, Van Scoyk M, Avasarala S, et al. The soft agar colony formation assay. J Vis Exp. 2014;(92):e51998. doi:10.3791/51998
- Bell E, Ivarsson B, Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci USA. 1979;76(3):1274–1278. doi:10.1073/pnas.76.3.1274
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467–477. doi:10.1021/bi00866a011
- Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci USA. 1986;83(8):2496–2500. doi:10.1073/pnas.83.8.2496
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
- Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
- Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
- Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
- Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029
