FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEML-R1X is a Microfluidics Suitable, ultra-filtered Dulbecco's Modified Eagle Medium (DMEM), Low Glucose, formulated without phenol red for organ-on-a-chip (OoC), microphysiological system (MPS), and microfluidic tissue culture applications. A quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off — five and a half times finer than the 0.22 µm membranes used in conventional sterile filtration — supporting unobstructed flow in sub-100 µm chip geometries.
- Low glucose (1.0 g/L) DMEM formulated without phenol red, with L-glutamine (584 mg/L), sodium pyruvate (110 mg/L), and sodium bicarbonate (3700 mg/L) buffering
- Processed through a validated quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — 5.5× finer than a 0.22 µm sterile filter
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch, not per unit
- Phenol red–free formulation eliminates estrogen-agonist and autofluorescence interference for hormone-sensitive assays and live-cell imaging
- Bicarbonate-buffered (3700 mg/L NaHCO3); requires approximately 10% CO2 to maintain pH 7.4 (Henderson–Hasselbalch)
- Manufactured under an ISO 13485:2016 quality management system; final QC and release testing performed at Diagnocine, Totowa, NJ
- Custom pH, glucose concentration, salts, and nutrient adjustments available on request
- Glucose1000 mg/L (1.0 g/L, Low Glucose)
- L-Glutamine584 mg/L
- Sodium Pyruvate110 mg/L
- Sodium Bicarbonate3700 mg/L
- Phenol RedNot added
- pH (USP <791>)7.4
- Osmolality (USP <785>)310–350 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Storage / Shelf Life2–8°C, protect from light / 12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma (0.2–0.3 µm diameter), subvisible particulates, and endotoxin fragments that accumulate inside microfluidic channels, clog chip geometries, and corrupt sensor readings and metabolic signals. FluxMPS™ was designed specifically for these failure modes.
Microchannel-safe purity
0.04 µm final filtration removes sub-micron particles that clog channels below 100 µm. USP <788> particulate compliance supports safe perfusion in demanding chip geometries.
Total metabolic control
Low glucose (1.0 g/L) base with defined pyruvate, bicarbonate, and no phenol red allows researchers to control carbon sources precisely for Warburg-effect and metabolic flux studies.
Ultrapure-grade water
Every batch is prepared using Ultrapure Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696) formulated for low trace-metal and organic-carbon (TOC) content.
Low background for imaging
This phenol red–free formulation removes autofluorescent dye interference, delivering a lower particulate baseline for confocal, widefield, and biosensor imaging on chip.
Rich, stable nutrient profile
4× BME amino acid & vitamin concentrations, micro-batch manufacturing, and per-lot QC support reproducible cell growth across multi-day perfusion experiments.
Customization on demand
pH, glucose concentration, salts, HEPES, and nutrient composition are adjustable per your protocol. Contact support@diagnocine.com to specify your formulation.
Quadruple-stage filtration system
A validated, four-stage filtration train reaches a final 0.04 µm polish under aseptic fill conditions. The train runs as two dedicated prefilter + final-filter pairs, each 0.04 µm final filter protected by its own 0.1 µm prefilter.
-
1
0.1 µm Prefiltration I
Removes large aggregates, cell debris, and contaminants, protecting the first 0.04 µm final filter cartridge from early fouling.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
A second, dedicated prefilter protecting the second 0.04 µm final filter cartridge — not a polish of Stage 2 effluent, but redundant protection for Stage 4.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter prior to aseptic fill & finish in a validated ISO Class 5 (Class 100) laminar-flow workstation.
Filtration architecture
FluxMPS™ DCP-DMEML-R1X runs two prefilter + final-filter pairs in series, giving full redundancy against membrane breakthrough while reaching a pore size well below conventional 0.22 µm sterile filtration.
(0.1 µm ×2 + 0.04 µm ×2)
© Diagnocine® — DCP-DMEML-R1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEML-R1X supports demanding cell culture platforms — from single-channel microfluidic chips to multi-organ body-on-a-chip systems — where media purity, metabolic precision, and optical clarity matter.
Automated Bioreactors & Robotics
An optional MPS Grade variant — a 0.01 µm (10 nm) ultra nano-filtered, six-stage cascade — is available on request for automated bioreactor perfusion systems, robotic liquid handlers, and long-duration closed-loop platforms where trace particulates cause valve failure or sensor drift.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC methods
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves, peristaltic pump tubing, and inline optical sensors
- Extended Perfusion Stability: Supports consistent nutrient delivery over weeks-long culture without in-line filter replacement
Inquiry Required: The 0.01 µm MPS Grade product is available by request. Contact support@diagnocine.com to request this variant for your automated system.
Micro Physiological System (MPS) & Chip
Ultra-clean, 0.04 µm–filtered media supports laminar flow fidelity in complex multi-organ chip architectures without early channel fouling.
Warburg Effect & Metabolic Research
Low glucose (1.0 g/L) base with defined pyruvate content enables control of aerobic glycolysis for Warburg phenotype studies.
iPSC-Derived Models
Ultra-low endotoxin (< 0.05 EU/mL) and 0.1 µm mycoplasma-retentive filtration make this medium suitable for sensitive iPSC-differentiation protocols.
Endothelial & Primary Cells
Particle-controlled, endotoxin-tested DMEM supports HUVEC and primary hepatocyte monolayer integrity for barrier-function assays and TEER monitoring.
Metabolic Flux Analysis
Defined low-glucose formulation provides a controlled metabolic background for ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red–free medium; this formulation contains 3700 mg/L sodium bicarbonate.
Microscopy & Optical Sensing
This phenol red–free, ultra-low-particulate formulation supports confocal imaging, biosensor arrays, and TEER measurements on chip with reduced background interference.
Analytical release specifications
Every lot is released against the full specification matrix below. CoA available on request: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] Low Glucose, [+] Sodium Pyruvate | [-] Phenol Red |
| Appearance | Pale yellow to colorless, clear solution (no phenol red indicator dye) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310–350 mOsm/kg H2O |
| Glucose | 1000 mg/L (1.0 g/L, Low Glucose) |
| L-Glutamine | 584 mg/L |
| Sodium Pyruvate | 110 mg/L |
| Phenol Red | Not added |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification — see § Manufacturing & Compliance) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protect from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | ~10% CO2 (bicarbonate-buffered at 3700 mg/L NaHCO3; calculated via Henderson–Hasselbalch for target pH 7.4) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
FluxMPS™ DMEM is a modification of Basal Medium Eagle (BME) containing 4× BME concentrations of amino acids and vitamins, plus glycine, serine, and ferric nitrate. Total ingredients: 32. Every ingredient listed below is present in the released product; CAS numbers provided for traceability.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous, multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016–certified quality management system. Final packaging, QA, and testing are conducted at the Diagnocine R&D and Quality Testing Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
All formulations use Ultrapure Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696), formulated for low trace-metal and organic-carbon (TOC) content.
ISO Class 5 Fill & Finish
Final aseptic fill performed in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity at the point of fill.
Micro-Batch Precision
Small-batch production with full per-lot traceability. Each batch is individually tested and released against the specification below — no blending of lots. A Certificate of Analysis is issued for every batch.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay. Assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL. Tested per manufacturing batch.
Particulate — USP <788> Method 1
Light obscuration particle count test. Release limits: ≤25/mL (≥10 µm) and ≤3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target range: 310–350 mOsm/kg H2O.
Documentation & CoA
Full Certificate of Analysis with raw-material traceability, in-process records, and final-release test results available for every batch upon request.
How DCP-DMEML-R1X compares
FluxMPS™ DCP-DMEML-R1X vs. conventional 0.22 µm–filtered DMEM formulations.
| Parameter | DCP-DMEML-R1X (FluxMPS™) | Conventional DMEM (0.22 µm filtered) |
Standard Alt. DMEM (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable (0.04 µm final cut-off) | Not tiered | Not tiered |
| No Phenol Red — eliminates estrogen-agonist interference for hormone-sensitive cell lines, live-cell imaging, and optical biosensor applications | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 µm, Stages 1 & 3) | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel Not specified | cancel Not specified |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Compatible with sub-100 µm geometries | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed formulation | cancel Fixed formulation |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEML-R1X, a Microfluidics Suitable DMEM formulation.
Supporting literature
Key peer-reviewed publications supporting the use of ultra-filtered, Microfluidics Suitable media in organ-on-a-chip, microfluidic, and metabolic research applications.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier with endothelial cells, pericytes and astrocytes. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
