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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o L-Glutamine, Sodium Pyruvate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o L-Glutamine, Sodium Pyruvate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o L-Glutamine, Sodium Pyruvate: 1X Liquid
FluxMPS™ DCP-DMEML-QP1X is a Microfluidics Suitable, Quadruple-stage ultra-filtered DMEM Low Glucose formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. L-glutamine and sodium pyruvate are omitted so researchers can define their own nitrogen and secondary carbon sources.
- Low glucose (1.0 g/L) DMEM base with L-glutamine and sodium pyruvate omitted, giving full control over nitrogen and secondary carbon sources for Warburg-effect and metabolic flux studies
- Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, reaching a 0.04 µm final polish
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Sodium bicarbonate–buffered (3700 mg/L) with phenol red (15.900 mg/L) present as a visual pH indicator; a 10% CO2 incubator is recommended to maintain pH 7.4
- 31 composition components across four groups (Inorganic Salts, Amino Acids, Vitamins, Others), released with full lot traceability
- Manufactured under an ISO 13485:2016 quality management system; final packaging and QC at Diagnocine, Totowa, NJ
- Custom pH, glucose, salts, and nutrient adjustments available on request — contact support@diagnocine.com
- Glucose1000 mg/L (1.0 g/L, Low Glucose)
- L-GlutamineNot included
- Sodium PyruvateNot included
- pH (USP <791>)7.4
- Osmolality (USP <785>)310–350 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma (0.2–0.3 µm diameter), subvisible particulates, and endotoxin fragments that accumulate inside microfluidic channels, clog chip geometries, and corrupt sensor readings and metabolic signals. FluxMPS™ is built for these failure modes.
Microchannel-safe purity
0.04 µm final filtration removes sub-micron particles that clog channels below 100 µm. USP <788> particulate compliance supports safe perfusion in fine chip geometries.
Total metabolic control
Low glucose (1.0 g/L) base with user-defined glutamine, pyruvate, and bicarbonate lets you precisely set carbon and nitrogen sources for Warburg-effect and metabolic flux studies.
Ultrapure-grade water
Every batch is prepared using Ultrapure Type 1 water (18.2 MΩ·cm), with trace-metal and total organic carbon (TOC) control supporting sensitive cell-based assays.
Low background for imaging
Ultra-low particulate baseline supports cleaner confocal, widefield, and biosensor measurements on chip.
Rich, stable nutrient profile
4× BME amino acid & vitamin concentrations, micro-batch manufacturing, and lot-to-lot QC support reproducible cell growth across multi-day perfusion experiments.
Customization on demand
pH, glucose concentration, salts, HEPES, and nutrient composition are adjustable per your protocol. Contact support@diagnocine.com to specify your formulation.
Quadruple-stage filtration system
A validated four-stage filtration train reaching a final 0.04 µm polish under aseptic fill conditions — engineered to reduce particulate load and mycoplasma-sized contaminants beyond conventional 0.22 µm filtration.
-
1
0.1 µm Prefiltration I
Removes large aggregates, cell debris, and protein aggregates. Protects the first 0.04 µm final filter cartridge and extends its service life.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass through a standard 0.22 µm filter, including material within the 0.2–0.3 µm mycoplasma diameter range.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge and providing redundancy against upstream filter breakthrough.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill and finish performed in a validated ISO Class 5 (Class 100) laminar-flow workstation.
Performance vs. conventional media
0.22 µm media by
particulate count
pore size — sub-mycoplasma
polishing
© Diagnocine® — DCP-DMEML-QP1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEML-QP1X supports demanding cell culture platforms — from single-channel microfluidic chips to multi-organ body-on-a-chip systems — where media purity, metabolic precision, and optical clarity matter.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant is available on request for automated bioreactor perfusion systems, robotic liquid handlers, and long-duration closed-loop platforms where even trace particulates cause valve failure or sensor drift.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC methods
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves, peristaltic pump tubing, and inline optical sensors
- Extended Perfusion Stability: Consistent nutrient delivery over weeks-long culture without filter replacement in the chip circuit
Inquiry Required: The 0.01 µm MPS Grade product is a custom order. Contact support@diagnocine.com to request this variant for your automated system.
Micro Physiological System (MPS) & Chip
0.04 µm–filtered media helps prevent microchannel clogging and supports laminar flow fidelity in complex multi-organ chip architectures.
Warburg Effect & Metabolic Research
Low glucose (1.0 g/L) base with user-defined nitrogen sources enables control of aerobic glycolysis, supporting Warburg phenotype studies.
iPSC-Derived Models
Ultra-low endotoxin (< 0.05 EU/mL) and 0.1 µm/0.04 µm mycoplasma-retentive filtration make this medium suitable for sensitive iPSC-differentiation protocols.
Endothelial & Primary Cells
Low-particulate, endotoxin-controlled DMEM supports HUVEC and primary hepatocyte monolayer integrity, useful for barrier-function assays and TEER monitoring.
Metabolic Flux Analysis
Defined low-glucose formulation with omitted glutamine provides a clean metabolic background for ¹³C isotope tracing and extracellular flux measurement. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate count provides a cleaner baseline for confocal imaging, biosensor arrays, and TEER measurements on chip.
Analytical release specifications
Every lot is released against the full specification matrix below. CoA available on request: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Low Glucose | [-] L-Glutamine, [-] Sodium Pyruvate |
| Appearance | Straw to red, clear solution (phenol red pH indicator) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310–350 mOsm/kg H2O |
| Glucose | 1000 mg/L (1.0 g/L, Low Glucose) |
| L-Glutamine | Not included |
| Sodium Pyruvate | Not included |
| Phenol Red | 15.900 mg/L |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | 10% CO2 incubator recommended (3700 mg/L sodium bicarbonate formulation) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
FluxMPS™ DMEM is a modification of Basal Medium Eagle (BME) containing 4× BME concentrations of amino acids and vitamins, plus glycine, serine, and ferric nitrate. Total: 31 components across four composition groups (Inorganic Salts, Amino Acids, Vitamins, Others), presented in three tabs below (Vitamins and Others share the third tab). Every ingredient is released per lot; CAS numbers provided where known.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 109.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final packaging, QA, and testing are conducted at the Diagnocine R&D and Quality Testing Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
All formulations use Ultrapure Type 1 water (18.2 MΩ·cm), with trace-metal and total organic carbon (TOC) control supporting sensitive cell-based assays.
ISO Class 5 Fill & Finish
Final aseptic fill performed in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity and reducing environmental contamination at the point of fill.
Micro-Batch Precision
Small-batch production with full per-lot traceability. Each batch is individually tested and released. A Certificate of Analysis is issued for every batch.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL. Tested per manufacturing batch.
Particulate — USP <788> Method 1
Light obscuration particle count. Release limits: NMT 25/mL (≥10 µm) and NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target range: 310–350 mOsm/kg H2O.
Documentation & CoA
Full Certificate of Analysis with raw-material traceability, in-process records, and final-release test results available for every batch upon request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEML-QP1X compares
FluxMPS™ DCP-DMEML-QP1X vs. conventional 0.22 µm–filtered DMEM formulations.
| Parameter | DCP-DMEML-QP1X (FluxMPS™) | Conventional DMEM (0.22 µm filtered) |
Standard Alt. DMEM (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not graded | Not graded |
| No L-Glutamine and no Sodium Pyruvate — researcher-defined nitrogen and energy sources | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1/0.04 µm) | cancel No | cancel No |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel Not specified | cancel Not specified |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed formulation | cancel Fixed formulation |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEML-QP1X.
Supporting literature
Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip, microfluidic, and metabolic research applications.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
