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FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose: 1X Liquid

Product#: DCP-DMEMHGFA1X
$34.10
DCP-DMEMHGFA1X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
Cell Culture Media
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate

FluxMPS™ DCP-DMEMHGFA1X is a Microfluidics Suitable, quadruple-stage ultra-filtered Dulbecco’s Modified Eagle Medium formulated with 4,500 mg/L D-glucose, L-glutamine, sodium pyruvate, sodium bicarbonate, phenol red, and folic acid — engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS) where particulate contamination corrupts biosensor signals and blocks micron-scale conduits. Filtered through a validated 0.1 µm ×2 + 0.04 µm ×2 cascade (four passes total) and released to an endotoxin specification of < 0.05 EU/mL, this medium is formulated as a ready-to-use, full-nutrient high-glucose DMEM for chip-based biology.[1,2]

  • Quadruple-stage filtration train: 0.1 µm (Prefiltration I) → 0.04 µm (Final filtration I) → 0.1 µm (Prefiltration II) → 0.04 µm (Final filtration II — Polish)
  • Endotoxin release specification < 0.05 EU/mL (LAL assay, USP <85> BET), controlled and tested per manufacturing batch
  • Full-nutrient high-glucose formulation: 4,500 mg/L D-Glucose, 584 mg/L L-Glutamine, 110 mg/L Sodium Pyruvate, 3,700 mg/L Sodium Bicarbonate, and 4 mg/L Folic Acid
  • Sodium bicarbonate-buffered at 3.7 g/L — formulated for a 10% CO2 incubator environment
  • Manufactured under an ISO 13485:2016 quality management system; 21 CFR Part 820 (QMSR) aligned
  • Microfluidics Suitable, 0.04 µm final cut-off — engineered for organ-on-a-chip (OoC) and microphysiological systems (MPS) applications
  • Customizable formulation: pH, glucose concentration, salts, HEPES, folic acid level, and nutrient composition available on request — contact support@diagnocine.com
DCP-DMEMHGFA1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/ Folic Acid — 1X Liquid
  • D-Glucose4,500 mg/L
  • L-Glutamine584 mg/L
  • Sodium Pyruvate110 mg/L
  • Folic Acid4 mg/L (included)
  • pH (USP <791>)7.0 - 7.4
  • Osmolality (USP <785>)280 - 320 mOsm/kg
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration System0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8 °C, protected from light
  • Shelf Life12 months from manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard DMEM falls short

Conventional 0.22 µm-filtered DMEM can leave behind subvisible particulates and mycoplasma-scale debris (0.2–0.3 µm) that accumulate at chip inlets, corrupt TEER and electrochemical biosensor baselines, and block gravity-fed perfusion in microchannels as narrow as 10–100 µm. FluxMPS™ DCP-DMEMHGFA1X is filtered through a four-stage nano-filtration train specifically to address these failure modes.[3,4]

filter_alt

Microchannel-Safe Purity

A final 0.04 µm filter stage reduces particulate and bacterial-fragment burden relative to single-pass 0.22 µm filtration, supporting use in narrow chip geometries where particulate accumulation is a known failure mode.

target

Total Metabolic Control

4,500 mg/L glucose powers energy-demanding cancer, neuronal, and cardiomyocyte models. Paired with 584 mg/L L-glutamine, sodium pyruvate, and folic acid, this formulation supports TCA-cycle and one-carbon metabolic flux studies.[5]

water_drop

Ultrapure-Grade Water

Prepared with Type 1 ultrapure water (18.2 MΩ·cm) controlled for trace-metal content and organic carbon (USP <643> TOC), supporting clean baselines for biosensor and imaging applications.

visibility

Low Background for Imaging

Reduced particulate baseline versus single-stage 0.22 µm media supports confocal microscopy, fluorescence-based biosensors, and TEER measurements where particulate debris — not optical clarity per se — is the primary confound.

science

Rich, Stable Nutrient Profile

4× BME amino acid and vitamin concentrations per DMEM specification, including glycine, serine, ferric nitrate, and folic acid — supporting rapidly dividing, proliferating cell populations in perfusion-based chip systems.[6]

tune

Customization on Demand

pH, glucose concentration, salts, HEPES, folic acid level, and overall nutrient composition are available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

DCP-DMEMHGFA1X is filtered through a four-stage 0.1 µm ×2 + 0.04 µm ×2 train, alternating a dedicated prefilter ahead of each final-filter cartridge. Each stage is independently processed before aseptic fill.

  1. 1

    0.1 µm Prefiltration I

    The first 0.1 µm membrane captures macro-aggregates, undissolved powder residues, and large particulate matter generated during formulation, protecting the downstream 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    The first 0.04 µm (40 nm) pass retains fine particulates and mycoplasma-sized debris (0.2–0.3 µm), delivering a polished stream ahead of the second prefilter/final-filter pair.

  3. 3

    0.1 µm Prefiltration II

    A second, independent 0.1 µm prefilter protects the second 0.04 µm cartridge, providing redundant capacity rather than polishing the effluent of stage 2.

  4. 4

    0.04 µm Final filtration II — Polish

    The final 40 nm polish stage is immediately followed by aseptic fill under laminar flow. Particulate burden is verified by USP <788> Method 1 (light obscuration) prior to release.

Filtration architecture vs. conventional 0.22 µm DMEM

Reducing final pore size from 0.22 µm to 0.04 µm and doubling the number of filtration passes is intended to reduce subvisible particulate carryover and support mycoplasma-scale debris retention relative to single-pass 0.22 µm filtration. Each batch is separately released against the endotoxin specification of < 0.05 EU/mL (see Manufacturing & Compliance).

4
Filtration passes
0.1 µm ×2 + 0.04 µm ×2
0.04
µm final filter
pore size
Sterility Assurance: Each lot is tested for absence of bacterial and fungal growth after 14 days of incubation per USP <71>. Mycoplasma-scale debris control is provided by 0.1 µm and 0.04 µm filtration (not independently tested per lot to USP <63>). CoA available at release — contact support@diagnocine.com.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEMHGFA1X DMEM High Glucose with Folic Acid Quadruple-Stage Filtration System diagram showing four sequential filter stages: 0.1 micron prefilter I, 0.04 micron final filtration I, 0.1 micron prefilter II, and 0.04 micron final filtration II polish, engineered for organ-on-a-chip, microphysiological systems, and microfluidic cell culture | Diagnocine
Figure 1. The FluxMPS™ Quadruple-Stage Filtration System: a sequential 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm train delivering polished DMEM into aseptic fill conditions.
© Diagnocine® — DCP-DMEMHGFA1X
Applications

Designed for next-generation cell culture platforms

DCP-DMEMHGFA1X supports advanced mammalian cell culture from classical monolayer experiments to perfused microphysiological systems. Its Microfluidics Suitable purity is especially relevant for models requiring low particulate background, biosensor precision, and multi-week perfusion runs. Folic acid inclusion supports applications requiring active folate-pathway metabolism and one-carbon cycle studies.[7,8]

Automated Bioreactors & Robotics

Next-Generation System Uptime

For fully automated perfusion bioreactors and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered MPS Grade variant of DCP-DMEMHGFA1X is available on inquiry, adding two further filtration stages beyond the 0.04 µm cut-off described above.

  • Total Particulate Exclusion: 10 nm filtration is intended to reduce subvisible debris and protect micro-valves and pneumatic actuators in automated systems
  • Valve & Sensor Protection: Reduced particulate burden helps limit valve seat fouling and optical sensor drift in closed-loop perfusion circuits
  • Extended Perfusion Stability: Cleaner medium is intended to reduce fouling of perfusion tubing and inline sampling ports

Inquiry Required: The 0.01 µm (10 nm) MPS Grade variant is produced on request. Contact support@diagnocine.com to request this grade.

Microfluidics

Micro Physiological System (MPS) & Chip

Microfluidics Suitable purity (0.04 µm final filtration) is designed for perfusion of organ-on-a-chip, tissue-on-a-chip, and body-on-a-chip platforms where particulates can block microchannels and corrupt TEER readouts.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

High glucose (4,500 mg/L) combined with folic acid supports aerobic glycolysis studies, Warburg-effect profiling, and one-carbon metabolism research in cancer cell lines. Pyruvate supplementation supports mitochondrial vs. glycolytic flux dissection.[5]

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Folic acid is an essential cofactor for rapidly proliferating iPSC-derived neurons, cardiomyocytes, and hepatocytes. Reduced particulate filtration is intended to limit particulate-associated stress in fragile differentiated progeny cultures.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

A low endotoxin release specification (< 0.05 EU/mL) is relevant for endothelial cultures, where LPS-level contamination can activate NF-κB, disrupt barrier integrity, and confound shear-stress experiments on vascular-on-a-chip models.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined, lot-certified composition supports stable isotope tracing and NMR metabolomics. Folic acid inclusion supports one-carbon and methionine-cycle flux studies. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Reduced particulate background relative to single-stage 0.22 µm media supports high-resolution confocal imaging, fluorescence-based biosensors, and TEER electrical measurements.

ConfocalBiosensorsTEER
Technical Specifications

Validated quality parameters

Every lot of DCP-DMEMHGFA1X is released against a specification panel covering physical/chemical properties, sterility, purity, storage, and regulatory traceability. Certificates of Analysis are available upon request from support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Folic Acid, [+] Phenol Red
Appearance Red-colored (phenol red indicator), clear solution
pH USP <791> 7.0 - 7.4
Osmolality USP <785> 280 - 320 mOsm/kg H2O
D-Glucose 4,500 mg/L (High Glucose)
L-Glutamine 584 mg/L
Sodium Pyruvate 110 mg/L
Phenol Red Present (pH indicator)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth at 14 days
Mycoplasma 0.1 µm and 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> M1 ≤ 6,000 particles/mL
Particulate ≥25 µm USP <788> M1 ≤ 600 particles/mL
Water Purity Ultrapure Type 1, 18.2 MΩ·cm; TOC-controlled USP <643>
Manufacturing Std. ISO 13485:2016; 21 CFR Part 820 (QMSR) aligned ISO
Fill Environment Aseptic fill & finish under laminar flow
Storage, Handling & Logistics
Parameter Specification
Storage Temperature 2 - 8 °C; protect from strong light
Freeze-Thaw Do not freeze; single-use aliquot recommended
Shelf Life 12 months from date of manufacture, unopened
Shipping Condition Refrigerated (cold pack, 2-8 °C)
CO2 Requirement 10% CO2 incubator recommended (formulated for 3.7 g/L NaHCO3)
Available Pack Sizes 500 mL, 1000 mL
Raw Materials & Regulatory Traceability
Parameter Specification
Raw Material Grade Cell-culture grade, lot-tested components
Traceability Full lot-to-lot CoA available; raw material lot records retained
Manufacturing QMS ISO 13485:2016 certified facility ISO 13485
Regulatory Alignment 21 CFR Part 820 (QMSR) aligned
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Production Method Quadruple-stage filtration; micro-batch formulation
Intended Use Research Use Only (RUO) — not for clinical or therapeutic use
Formulation

Full composition (mg/L)

DCP-DMEMHGFA1X contains 4× BME amino acid and vitamin concentrations per the standard DMEM specification, with folic acid included in the vitamins fraction. Total: 33 components across 4 categories. All values are per-lot release targets verified on the Certificate of Analysis.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine disodium salt 69847-55-8 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal 5 phosphate 54-47-7 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
D-Glucose 50-99-7 4500.000
i-Inositol 87-89-8 7.200
Phenol red sodium salt 34487-61-1 15.900
Sodium pyruvate 113-24-6 110.000
Custom Formulation Available: Need a different folic acid level, glucose concentration, HEPES buffer, adjusted pH, or modified salt profile? Diagnocine offers micro-batch custom formulation for MPS and chip-biology workflows. Contact support@diagnocine.com with your specifications.
Quality Assurance

ISO 13485:2016 manufacturing & multi-standard compliance

Every batch of DCP-DMEMHGFA1X is produced under a documented ISO 13485:2016 Quality Management System with 21 CFR Part 820 (QMSR) alignment, supporting the regulatory traceability required for tissue-chip platform qualification and reproducibility.

verified

ISO 13485:2016 QMS

Quality management system covering design control, batch records, raw material qualification, in-process testing, and final product release.

filter_alt

Quadruple-Stage Filtration

0.1 µm ×2 + 0.04 µm ×2 filtration train applied to every batch prior to fill.

biotech

Per-Lot QC & CoA

Appearance, pH, osmolality, endotoxin, sterility, and particulate testing performed on every batch; results reported on the Certificate of Analysis.

assignment

Micro-Batch Precision

Small, defined production batches support tighter lot-to-lot consistency for longitudinal chip experiments. Batch size is documented in the CoA.

Endotoxin — USP <85> BET

LAL assay performed on every batch. Assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light-obscuration particle counting performed on each batch, against ≥10 µm and ≥25 µm thresholds.

Osmolality — USP <785>

Freezing-point depression osmometry per USP <785>. Release range: 280-320 mOsm/kg H2O.

Documentation & CoA

Certificate of Analysis including appearance, pH, osmolality, endotoxin, sterility, particulate count, and lot traceability available at release.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request — contact support@diagnocine.com.
Product Comparison

How DCP-DMEMHGFA1X compares

The table below contrasts FluxMPS™ DCP-DMEMHGFA1X against two categories of conventional 0.22 µm-filtered DMEM High Glucose products.

Parameter DCP-DMEMHGFA1X
(FluxMPS™)		 Conventional DMEM
w/ Folic Acid
(0.22 µm filtered)		 Standard DMEM
High Glucose
(0.22 µm filtered)
Grade Microfluidics Suitable Standard reagent grade Standard reagent grade
Folic Acid Included check_circle 4 mg/L check_circle 4 mg/L cancel Absent
Final Filtration Pore Size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Number of Filtration Stages 4 passes 1 pass 1 pass
Mycoplasma-Retentive Filtration check_circle 0.1 & 0.04 µm stages cancel Not addressed cancel Not addressed
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP Particulate Compliance check_circle USP <788> Method 1 tested cancel Not tested cancel Not tested
Water Quality Ultrapure Type 1, 18.2 MΩ·cm Tissue culture grade Tissue culture grade
Manufacturing QMS ISO 13485:2016 certified ISO 9001 (typical) ISO 9001 (typical)
Microfluidic Channel Compatibility check_circle Microfluidics Suitable cancel Not validated cancel Not validated
Custom Formulation check_circle Available on request cancel Fixed formulation cancel Fixed formulation

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about DCP-DMEMHGFA1X formulation, filtration, and use in advanced cell culture platforms.

Yes. DCP-DMEMHGFA1X is filtered through a four-stage 0.1 µm ×2 + 0.04 µm ×2 train intended to reduce particulate carryover that can block microchannels, and is released against an endotoxin specification of < 0.05 EU/mL, relevant for cytokine-sensitive OoC and MPS models. The folic acid inclusion supports folate-dependent epithelial and neural cell types used in gut-on-a-chip, brain-on-a-chip, and body-on-a-chip systems.[1,4]
Standard 0.22 µm filtration is a single-pass process. DCP-DMEMHGFA1X passes through four independent membranes — two at 0.1 µm and two at 0.04 µm — arranged as two prefilter/final-filter pairs in series. This provides redundant capacity and a finer final cut-off than a single 0.22 µm pass.[2,3]
Folic acid (4 mg/L) is a B-vitamin involved in one-carbon metabolism, nucleotide biosynthesis, and DNA methylation. Many proliferating cell lines, including iPSC-derived neurons and certain cancer lines, have an active folate requirement. DCP-DMEMHGFA1X includes folic acid at the standard DMEM concentration. Users working with folate-restricted models should request a folic-acid-free custom formulation via support@diagnocine.com.[8,9]
Yes. DCP-DMEMHGFA1X contains sodium bicarbonate (3.7 g/L) as its primary pH buffer and phenol red as a visual pH indicator. This bicarbonate concentration is formulated for a 10% CO2 incubator environment to maintain pH within the 7.0-7.4 specification. Use outside a matched CO2 environment will cause the medium to drift alkaline. Contact support@diagnocine.com to inquire about HEPES-buffered custom variants.
Yes. Like all DMEM formulations, DCP-DMEMHGFA1X does not contain proteins, lipids, or growth factors and requires supplementation (typically 5-10% FBS or an appropriate serum-free system) per your cell line’s requirements. Serum and protein-containing additions should be pre-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition; a 0.04 µm membrane is not appropriate for serum-containing supplements.
Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested by an LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. The result is reported on the Certificate of Analysis.[3]
Yes. A lot-specific Certificate of Analysis is generated for every production batch and is available upon request. The CoA includes product name and catalog number, lot number and manufacture/expiry dates, appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility result (USP <71>), particulate count (USP <788> Method 1), filtration system verification, and authorized quality-release signature. Request by contacting support@diagnocine.com with your lot number.
Scientific References

Supporting literature

Peer-reviewed references supporting the science behind Microfluidics Suitable cell culture media, organ-on-a-chip applications, microfluidic perfusion, and media purity for chip-based biological models.

  1. Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662-1668. doi:10.1126/science.1188302
  2. Bhattacharya M, Malinen MM, Lauren P, Lou YR, Kuisma SW, Kanninen L, et al. Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture. J Control Release. 2012;164(3):291-298. doi:10.1016/j.jconrel.2012.06.039
  3. Berthier E, Young EW, Beebe D. Engineers are from PDMS-land, biologists are from Polystyrenia. Lab Chip. 2012;12(7):1224-1237. doi:10.1039/c2lc20982a
  4. Leung CM, de Haan P, Ronaldson-Bouchard K, Kim GA, Ko J, Rho HS, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
  5. Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309-314. doi:10.1126/science.123.3191.309
  6. Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130(3373):432-437. doi:10.1126/science.130.3373.432
  7. Ribas J, Pawlikowska J, Rouwkema J. Microphysiological systems: analysis of the current status, challenges and commercial future. Microphysiological Syst. 2021;5:6. doi:10.21037/mps-20-7
  8. Ducker GS, Rabinowitz JD. One-carbon metabolism in health and disease. Cell Metab. 2017;25(1):27-42. doi:10.1016/j.cmet.2016.08.009
  9. Stover PJ, Field MS. Vitamin B-9. Adv Nutr. 2011;2(6):536-537. doi:10.3945/an.111.000786
  10. van der Meer AD, van den Berg A. Organs-on-chips: breaking the in vitro impasse. Integr Biol. 2012;4(5):461-470. doi:10.1039/c2ib00176d

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