Anti human EDG3/S1PR3 mouse monoclonal antibody(ProteinA purified), 1mg/mL

Product#: FNK-PP-AS055-0C
$866.92
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Anti human EDG3/S1PR3 mouse monoclonal antibody(ProteinA purified), 1mg/mL


Cat. No.: FNK-PP-AS055-0C

Size: 100μl

Storage: -20℃


Description:
 

Serum-free hybridoma supernatant (NS-1 × MRL/lpr spleen) from a mouse immunized with egg-yolk lecithin/monophosphoryl lipid A (20:1) liposomereconstituted human S1PR3 (aa 2–330).


Specifications:
 

S1PR3: Sphigosine-1-Phosphate Receptor 3

Clone No. AS055

Lot. B-1

Concentration: 1mg/mL

Ig Class: G2a

Genbank: NM_005226.4

Specificity: This antibody specifically recognizes human PNR. Not yet tested in other species.

Purification: Ammonium sulfate fractionation

Formulation: Physiologic saline with 0.1 % NaN3.


Application / Recommended Concentration: 

In order to obtain the best results, optimal working dilutions should be determined by each individual user.

Western Blot Not yet tested
Non reducing Western Blot Not yet tested
ELISA Not yet tested
Immunoprecipitation Not yet tested
Supershift Assay Not yet tested
Flow Cytometry 5-10 mg/mL
Chromatin immunoprecipitation Not yet tested
Immunohistochemistry Decide by use
 
Reflexive expression of S1PR3 (normalized GAPQH)









 

Cama1 siRNA treated Cell Primary Antibody Blue: AS055 10 mg/mL, Red: isotype 10 mg/mL Secondary Antibody AF488-Goat anti mouse IgG (x1000)
Cama1 siRNA treated Cell

Primary Antibody

Blue: AS055 10 mg/mL,

Red: isotype 10 mg/mL

Secondary Antibody

AF488-Goat anti mouse IgG (x1000)

 


Notes:

Store at 2 - 8 ºC up to one month. For long-term storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in a frost-free freezer is not recommended.

Sodium azide may react with lead and copper plumbing to form explosive metal azides. Flush with large amounts of water during disposal.


Reference:

Maeda S, et al. Sci Adv. 2021; 7(24): eabf5325

FOR RESEARCH ONLY. NOT FOR USE IN HUMANS.

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