Signal Booster Immunostain

Product#: BCLIS
$135.00

Solution Type

  • FMS
  • F
  • M
  • S
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Signal Booster Immunostain


Cat No: BCLIS
Storage: 4°C 


Description

Signal Booster Immunostain is a product specially developed for immunostaining. It is a reagent to improve sensitivity and high background. This reagent consists of 3 solutions, each with different characteristics. 

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Principle

The main component of the signal booster series is a polymer and a component that has a blocking action. By changing the physicochemical properties of the antigen and antibody, the polymer increases the frequency of contact between them and enhances the specific antigen-antibody reaction. On the other hand, a blocking component reduces non-specific binding. As a result, the background is lowered and the specific antigen-antibody reaction is enhanced.


Product Types 

1. Enhances signal and reduces background.
2. Any kind of antibody can be used (Note).
3. Easy to use, simply dilute the antibody at the time of reaction with this reagent.
4. Cheaper than existing products.
(Note: The effect of this reagent may not be sufficient depending on the characteristics of the antibody.)

 
Product No. Product Name Capacity Price
BCL100_50 mL Signal Booster Solution A&B set Solution A & B 50mL each $50.00
BCL100_100 mL Solution A & B 100mL each $100.00
BCL100_250 mL Solution A & B for each 250mL $180.00
 
BCL110_A-100 mL Signal Booster Solution 100 mL $55.00
BCL110_B-100 mL 100 mL $55.00
BCL110_A-250 mL 250 mL $95.00
BCL110_B-250 mL 250 mL $95.00
 
BCL-SBN_250 mL Signal Booster Neo 250 mL $150.00
BCL-SBN_500 mL 500 mL $265.00
 
BCLIS_FMS Signal Booster Immunostain 10 mL each $135.00
BCLIS_F Solution F, 20 mL $202.00
BCLIS_M Solution M, 20 mL $202.00
BCLIS_S Solution S, 20 mL $202.00


Features

  • Easy to use
    • You can experience the performance by simply exchange the ordinary antibody-dilution buffer to "Signal Booster Immunostain" .
  • Wide variety of antibody can be used
    • Better performence of Signal Booster Immunostain can be seen regardless the origin and subclass of the antibody as pronciple.
  • Stronger signal and lower background as compared to ordinary method
    • As compared to the ordinary method using detergent-containing buffer solution, you can get stronger signals with lower background.
  • Choose the best solution for your system
    • There are three types of Signal Booster Immunostain solution.
      • Solution F
        • The solution is desinged specially to reduce the background more, and suitable for observing the detail of preparations using relatively good antibodies.
      • Solution M
        • The solution shows the medium characteristics of Solution F and S, and suitable for first trial.
      • Solution S
        • The solution is designed to get more strong signal, and suitable for getting stronger signal regardless of relatively high background.
(Warning : The characteristics of each solution above described is only the general one, the performance of each solution differs depending on the nature of antibodies you use. Please examine all the three solutions to select the best one.)


Example Data
 
Staining of Cdk4 (mouse brain paraffin section)

1st Ab: anti-Cdk4(rabbit poly) diluted with Signal Booster (SBI) solution F, PBS or Product A
Fixation:4% parafolmaldehyde in PBS
Blocking:5%FBS in PBS
Stained by ABC (for rabbit) method.
 
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Staining of p53 in A431 cells

1st Ab: anti-p53 (Rabbit poly, FITC conjugated) diluted with Signal Booster (SBI) solution M, PBS or Product A
Fixation:4% parafolmaldehyde in PBS
Detergent: 0.1% TritonX-100 in PBS
Blocking:5%FBS in PBS.
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Q & A 

Q: How long is the storage period?
All can be used without problems for more than one year if stored refrigerated.
Q: Is it available for phosphorylated proteins?
SB is available in some cases, but we recommend using SBN. In the case of SBN, we recommend using it in combination with the chemical blocking agent c-Block.
Q: Can it be used for immunohistochemistry?
We have developed Signal Booster Immunostain for immunohistochemistry. Please use it.
Q: How does it compare to other companies' products?
It is stronger than other companies' products with a two-component structure and has a low price. Only our company sells Signal Booster Neo with 100% chemical composition.
Q: Can I use it diluted?
Both Signal Booster and Signal Booster Neo have optimized solutions, so performance when diluted is not guaranteed.

 

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