Recombinant Anti-GAPDH antibody ( Mouse mAb )
Recombinant Anti-GAPDH antibody ( Mouse mAb )
General information
| Cat. No. | ：SB-GB15002-100 |
| Size | ：100 uL |
| Protein full name | ：Glyceraldehyde-3-phosphate dehydrogenase |
| Synonym | ：GAPDH , GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
| Immunogen | ：KLH conjugated synthetic peptide corresponding to Mouse GAPDH |
| Isotype | ：IgG1 |
| Purity | ：Affinity purification |
| Subcellular location | ：Cytoplasm, Nucleus, Membrane |
| Predicted MW. | ：36 kDa |
| Observed MW. | ：36 kDa |
| Uniprot ID | ：P16858 |
| Storage | ：Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Application
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 1000-1: 2000 |
heart, lung, liver, kidney, brain |
| IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | breast cancer, esophageal cancer, kidney |
Description
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to This long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.
 |
WB analysis of GAPDH (GB15002).
|
 |
IHC analysis of GAPDH (GB15002). |
 |
IHC analysis of GAPDH (GB15002).
Sample: Human esophageal cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1:1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
 |
IHC analysis of GAPDH (GB15002).
Sample: Mouse kindey (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1:1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
 |
IHC analysis of GAPDH (GB15002).
Sample: Rat kindey (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1:1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
 |
IHC analysis of GAPDH (GB15002).
Sample: Mouse testis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1:1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
 |
IHC analysis of GAPDH (GB15002).
Sample: Rat spinal cord (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1:1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h. |
Aliases for GAPDH Gene
- GeneCards Symbol: GAPDH 2
- Glyceraldehyde-3-Phosphate Dehydrogenase 2 3 4 5
- GAPD 3 4 5
- Peptidyl-Cysteine S-Nitrosylase GAPDH 3 4
- EC 1.2.1.12 4 48
- Epididymis Secretory Sperm Binding Protein Li 162eP 3
- Oct1 Coactivator In S Phase, 38 Kd Component 3
- Aging-Associated Gene 9 Protein 3
- OCAS, P38 Component 3
- HEL-S-162eP 3
- EC 2.6.99.- 4
- EC 1.2.1 48
- G3PD 3
