Ultrapure Water, Type 1, 1000ml, Non-Sterile

Q: What makes the filtration superior to standard single-pass filtered water?

FluxMPS Ultrapure Water, Type 1 is filtered through a 0.1 micron membrane twice and a 0.04 micron membrane twice, then deionized, removing finer particulates than a single conventional filtration pass.

Q: What pH should I expect, and can it be adjusted for my protocol?

As supplied non-pH-adjusted, this water measures pH 4.5 to 5. A NaOH pH-adjusted variant at pH 7.4 is also available for protocols requiring a physiological final pH.

ISO 13485 Certified Manufacturing

FluxMPS™ Ultrapure Water, Type 1, 1000 mL, Non-Sterile

FluxMPS™ Ultrapure Water, Type 1 is a quadruple-stage filtered, deionized, single-component aqueous reagent (H₂O, CAS 7732-18-5) manufactured for microfluidic, organ-on-a-chip (OoC), and molecular biology workflows that demand ultra-low particulate, nuclease-free water. Supplied non-sterile in a 1000 mL format, it is available non-pH-adjusted (pH 4.5–5) or NaOH pH-adjusted (pH 7.4) to match your protocol.

Quadruple-stage filtration: 0.1 µm membrane filtered twice and 0.04 µm membrane filtered twice
Deionized and 0.1 µm filtered for HPLC, GC, ICP-MS, cell culture, chemistry, and molecular studies
Tested electrolytic conductivity per ASTM standard: 0.05 µS/cm
Free of DNase, RNase, Protease & Endonucleases
Available non-pH-adjusted (pH 4.5–5) or NaOH pH-adjusted (pH 7.4)
Ultrapure Type 1 water classification (18.2 MΩ·cm)
Non-Sterile — For Research Use Only (RUO)

SKU: DCP-T1UH2O-1000N 41104206 UNSPSC Ultrapure Water, Type I

Ultrapure Water, Type 1 — 1000 mL, Non-Sterile

pH (Non-Adjusted)4.5–5
pH (Adjusted, NaOH)7.4
Conductivity (ASTM)0.05 µS/cm
CAS Number7732-18-5
Molecular FormulaH₂O
Filtration0.1 µm x2 + 0.04 µm x2
SterilityNon-Sterile
Nuclease/ProteaseDNase/RNase/Protease-Free
Water GradeType 1 (18.2 MΩ·cm)
Size1000 mL

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard ultrapure water falls short

Conventional single-pass filtered water can carry subvisible particulates, nucleases, and unstable pH readings into sensitive microfluidic channels and molecular assays. FluxMPS™ Ultrapure Water, Type 1 is built to remove those failure points.

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Microchannel-safe purity

Quadruple-stage 0.1 µm and 0.04 µm membrane filtration removes particulates before they can foul narrow microfluidic and OoC channels.

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Defined pH options

Choose non-pH-adjusted (pH 4.5–5) as supplied, or NaOH pH-adjusted (pH 7.4) for protocols requiring a physiological pH final mix.

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Ultrapure-grade water

Type 1 water classification (18.2 MΩ·cm) deionized and 0.1 µm filtered for demanding analytical and molecular biology use.

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Low background for sensitive assays

Free of DNase, RNase, Protease & Endonucleases, reducing background risk in molecular biology and general PCR workflows.

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Defined, traceable composition

Single-component reagent (H₂O, CAS 7732-18-5) tested to an electrolytic conductivity standard of 0.05 µS/cm (ASTM).

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pH options on demand

Non-pH-adjusted and NaOH pH-adjusted (pH 7.4) variants available — contact support@diagnocine.com for custom requests.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Ultrapure Water, Type 1 is produced through sequential 0.1 µm and 0.04 µm membrane filtration — filtered 0.1-micron membrane twice and 0.04-micron membrane twice — followed by deionization, removing finer particulates than a single-pass filtration step.

1

0.1 µm Pre-filtration I

Removes large particulates and aggregates, extending the life of downstream filtration membranes.
2

0.04 µm Pre-filtration II

Retains fine particulates ahead of final polishing and deionization.
3

0.1 µm Sterile-filtration I

Second-pass 0.1 µm redundancy for consistent particulate control.
4

0.04 µm Sterile-filtration II — Final Polish

Final 0.04 µm polish combined with deionization for HPLC-, GC-, ICP-MS-, and molecular-biology-grade purity.

Performance vs. conventional water

Sequential 0.1 µm and 0.04 µm filtration removes finer particulates than a single conventional filtration pass, while deionization and nuclease/protease screening support demanding analytical and molecular biology workflows.

0.04 µm

Final filtration stage

4

Total filtration stages

This Type 1 water is supplied Non-Sterile; it is not terminally sterilized or sterility-tested under USP <71>, and is intended for research workflows that do not require a sterile-labeled reagent.

FluxMPS DCP-T1UH2O-1000N quadruple-stage filtration diagram showing 0.1 micron and 0.04 micron membrane filtration stages for Ultrapure Type 1 water used in organ-on-a-chip, microfluidic, and molecular biology applications, Diagnocine — **Figure 1.** Quadruple-stage 0.1 µm and 0.04 µm membrane filtration architecture applied to FluxMPS™ Ultrapure Water, Type 1.
© Diagnocine® — DCP-T1UH2O-1000N

Applications

Where FluxMPS™ Ultrapure Water, Type 1 is used

Suited for HPLC, GC, ICP-MS, cell culture, chemistry, and molecular studies, as well as microfluidic and organ-on-a-chip (OoC) systems requiring ultra-low-particulate, nuclease-free Type 1 water.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered variant is available for automated bioreactor and robotic liquid-handling platforms where sub-0.04 µm particulate exclusion is required.

Total Particulate Exclusion
Valve & Sensor Protection
Extended Perfusion Stability

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade for your platform.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low-particulate water for priming and perfusing microfluidic channels.

OoCToCBoCLoCMPS

Sample Preparation

Wash, Dilution & Reconstitution

Nuclease-free base water for lysis, dilution, and reagent reconstitution.

LysisDilutionReconstitutionRinse

Stem Cell Biology

iPSC-Derived Model Handling

Low-background water for reagent preparation in iPSC-derived model workflows.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cell Perfusion

Ultrapure water for buffer and media preparation supporting perfusion protocols.

HUVECsHAECsPrimary hepatocytes

Immunoassays

ELISA, Blotting & Blocking

Reagent-grade water for wash and dilution steps in immunoassay workflows.

ELISAWestern blotIHCIF

Live-Cell Imaging

Microscopy & Optical Sensing

Low-particulate, low-background water supporting optical and sensor-based assays.

ConfocalBiosensorsTEER

For general PCR use only (i.e., DNA-based, gDNA, and abundant source of RNA for the gene of interest). Not recommended for delicate PCR applications (i.e., endpoint or qPCR). Packaged in Diagnocine Precision's Easy Tear-Off Tag format for convenient bench access.

Technical Specifications

Specifications at a glance

Key physical, purity, handling, and traceability parameters for FluxMPS™ Ultrapure Water, Type 1.

Physical & Chemical Parameters

Parameter	Specification
Formulation / Composition	Ultrapure Water (H₂O)
CAS Number	7732-18-5
pH, Non-Adjusted Grade	4.5–5
pH, NaOH-Adjusted Grade	7.4
Conductivity ASTM	0.05 µS/cm

Sterility, Purity & Safety Parameters

Parameter	Specification
Sterility	Non-Sterile
Nuclease / Protease Content	Free of DNase, RNase, Protease & Endonucleases
Water Purity Classification	Ultrapure Type 1 water (18.2 MΩ·cm)
Filtration System	0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
Deionization	Deionized, 0.1 µm filtered

Storage, Handling & Logistics

Parameter	Specification
Packaging Size	1000 mL
Air-Exposure Behavior	pH can fall toward 4.5 upon atmospheric CO₂ absorption; this does not indicate contamination
pH Meter Compatibility	Standard pH meters may show instability due to low conductance; a meter designed for ultra-pure water is recommended

Raw Materials & Regulatory Traceability

Parameter	Specification
Molecular Formula	H₂O
Applications	HPLC, GC, ICP-MS, Cell Culture, Chemistry & Molecular Studies
PCR Suitability	General PCR use only (DNA-based, gDNA, abundant RNA source); not for delicate PCR (endpoint or qPCR)
Manufacturing QMS ISO	ISO 13485:2016
Intended Use	Research Use Only (RUO); not for human consumption, therapeutic, or clinical use

Formulation

Full composition

FluxMPS™ Ultrapure Water, Type 1 is a single-component aqueous reagent.

Component	CAS Number	Concentration
Ultrapure Water (H₂O)	7732-18-5	Not specified

Non-pH-adjusted (pH 4.5–5) and NaOH pH-adjusted (pH 7.4) options are available. For custom pH formulations, contact support@diagnocine.com.

Quality Assurance

Manufacturing & compliance

FluxMPS™ Ultrapure Water, Type 1 is produced under Diagnocine Precision's quality systems.

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ISO 13485:2016 QMS

Manufactured under an ISO 13485:2016-aligned quality management system.

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Ultrapure Type 1 Water

Deionized and 0.1 µm filtered Type 1 water (18.2 MΩ·cm) as the base reagent.

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ISO Class 5 Fill & Finish

Fill operations conducted within controlled ISO Class 5 (Class 100) environments.

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Micro-Batch Precision

Lot-controlled production supporting consistent quality across batches.

Nuclease & Protease Screening

Confirmed free of DNase, RNase, Protease & Endonucleases.

Particulate Control

Quadruple-stage 0.1 µm and 0.04 µm membrane filtration.

Electrolytic Conductivity

Tested to ASTM standard: 0.05 µS/cm.

Documentation / CoA

Certificate of Analysis available on request.

Diagnocine Precision maintains rigorous quality control on every lot. For orders greater than 10 units, or to request a Certificate of Analysis, contact support@diagnocine.com.

Product Comparison

How DCP-T1UH2O-1000N compares

A look at FluxMPS™ Ultrapure Water, Type 1 against conventional filtered and distilled water grades.

Parameter	DCP-T1UH2O-1000N (FluxMPS™)	Conventional 0.22 µm-Filtered Water	Standard Distilled / Deionized Water
Final filtration pore size	0.04 µm	0.22 µm	Not filtered / variable
Number of filtration stages	4	1	0
Conductivity tested to ASTM standard	check_circle	cancel	cancel
Nuclease / protease-free	check_circle	cancel	cancel
Water classification	Type 1 (18.2 MΩ·cm)	Type 2/3 typical	Distilled (variable)
pH options available	Non-adjusted (4.5–5) & pH 7.4 adjusted	Single, non-adjusted only	Not controlled
Manufacturing QMS	ISO 13485:2016	Unspecified	Unspecified
Microfluidic / OoC channel compatibility	check_circle	cancel	cancel
Custom pH formulation available	check_circle	cancel	cancel

FAQ

Frequently asked questions

Common questions about FluxMPS™ Ultrapure Water, Type 1.

Yes. This ultra-low-particulate, nuclease-free Type 1 water is suited to microfluidic and organ-on-a-chip (OoC) workflows, as well as HPLC, GC, ICP-MS, cell culture, chemistry, and molecular studies.

FluxMPS™ Ultrapure Water, Type 1 is filtered through a 0.1-micron membrane twice and a 0.04-micron membrane twice, then deionized, removing finer particulates than a single conventional filtration pass.

As supplied non-pH-adjusted, this water measures pH 4.5–5. A NaOH pH-adjusted variant at pH 7.4 is also available for protocols requiring a physiological final pH.

Ultrapure Type 1 water has low conductance and can be unstable in standard pH meters; a meter designed for ultra-pure water is recommended. Exposure to air can cause pH to fall toward 4.5 as the water absorbs atmospheric CO₂, which does not indicate contamination.

This product is supplied as single-component Ultrapure Type 1 water (H₂O). It is commonly used as the base solvent for custom buffer preparation; contact support@diagnocine.com for custom formulation requests.

Endotoxin testing is not part of the standard specification for this Non-Sterile Type 1 water grade. Contact support@diagnocine.com to discuss lot-specific testing options.

Yes. Contact support@diagnocine.com to request a Certificate of Analysis for your lot.

Scientific References

Supporting literature

Curated literature relevant to ultrapure water quality, filtration, and microfluidic / organ-on-a-chip applications.

Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
Halpern M, Frenkel Y, Sowden R. Behavior of pH measurement in low ionic strength ultrapure water. Anal Chem Insights. 2007. doi:10.1177/117739010700200003
Light TS, Licht S, Bevilacqua AC, Morash KR. The fundamental conductivity and resistivity of water. Electrochem Solid-State Lett. 2005. doi:10.1149/1.1836121
Huh D, Hamilton GA, Ingber DE. From 3D cell culture to organs-on-chips. Trends Cell Biol. 2011. doi:10.1016/j.tcb.2011.09.005
Rios A, Zougagh M. Sample preparation for micro total analytical systems (µTAS). Anal Bioanal Chem. 2015. doi:10.1007/s00216-015-8547-4
Zhao Q, et al. Nuclease contamination control in molecular biology reagent water. Anal Biochem. 2012. doi:10.1016/j.ab.2012.02.005
Kim JJ, et al. Endotoxin and particulate control in laboratory water purification systems. J Lab Autom. 2013. doi:10.1177/2211068212460237
Bruus H. Theoretical Microfluidics. Oxford University Press. 2008. doi:10.1093/oso/9780199235094.001.0001
Whitesides GM. The origins and the future of microfluidics. Nature. 2006. doi:10.1038/nature05058
Green MR, Sambrook J. Preparation of reagents and buffers used in molecular cloning. Cold Spring Harb Protoc. 2020. doi:10.1101/pdb.top100438