Normal Saline (Sodium Chloride), 0.9% (w/v), Sterile

FluxMPS™ Normal Saline (Sodium Chloride), 0.9% (w/v), Sterile

An MPS-grade, physiological-pH normal saline formulated to 0.9% (w/v) Sodium Chloride in ASTM Type 1 Ultrapure Water. Quadruple-stage 0.1 µm / 0.04 µm membrane filtration delivers an isotonic, non-toxic solution suited to microchannel priming, cell washing, tissue hydration, and reagent preparation across microfluidic and organ-on-a-chip (OoC) workflows.

• Quadruple-stage filtration: 0.1 µm membrane twice and 0.04 µm membrane twice for microchannel-safe purity
• Formulated to physiological default pH 7.4 (unadjusted normal saline typically ranges pH 4 to 6)
• 0.9% (w/v) Sodium Chloride in ASTM Type 1 Ultrapure Water (0.05 µS/cm), deionized
• Isotonic, non-toxic solution that minimizes disturbance to healing tissue and cultured cells
• Tissue Culture & Molecular Biology grade, manufactured under ISO 13485-certified, CE-approved facilities
• Customizable pH (7.4 default or approximately 4 to 6), concentration, and additive content on request

Engineered where standard saline falls short

Conventional 0.22 µm-filtered saline can carry finer particulates, unadjusted acidic pH, and undocumented water quality into sensitive microfluidic and cell-based work. FluxMPS™ Normal Saline addresses each failure mode with defined pH, quadruple-stage filtration, and ultrapure water.

Quadruple-stage filtration system

DCP-NSALINES1X is filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice, a four-pass architecture that supports mycoplasma-safe purity for microchannel and sensitive laboratory applications.

1. 1

   0.1 µm Pre-filtration I

   First pass through a 0.1 µm membrane removes large particulate and aggregate material, protecting downstream filters from early loading.

2. 2

   0.04 µm Pre-filtration II

   First pass through a 0.04 µm membrane retains finer particulates and bioburden ahead of the final sterile passes.

3. 3

   0.1 µm Sterile-filtration I

   Second 0.1 µm pass provides redundant particulate control ahead of final polishing.

4. 4

   0.04 µm Sterile-filtration II — Final Polish

   Second 0.04 µm pass, performed in a sterile environment, provides the final polish. The smallest mycoplasma species measure about 0.2 microns, so this stage helps guard against mycoplasma-scale contamination.

Where FluxMPS™ Normal Saline is used

Normal Saline is used in the laboratory for multiple purposes, including washing cells, pathological work, treating or transporting tissues, hydration of cells, preparing reagents, and adjusting electrolyte disturbances.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra-filtered variant is available on inquiry for automated bioreactor and robotic liquid-handling platforms where finer particulate exclusion is required.

• Total Particulate Exclusion for closed-loop automated systems
• Valve & Sensor Protection against fine particulate fouling
• Extended Perfusion Stability in long-duration automated runs

Inquiry Required: the 0.01 µm ultra-filtered grade is a customization option; contact support@diagnocine.com to request it.

Specifications

Measured and declared values for DCP-NSALINES1X.

Full composition

Two-component formulation released per lot at the DiagnoCine R&D and Quality Testing Center.

Manufacturing & compliance

Manufactured under ISO 13485-certified and CE-approved facilities, with final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.

How DCP-NSALINES1X compares

A comparison of FluxMPS™ Normal Saline against conventional 0.22 µm-filtered saline.

Frequently asked questions

Common questions about DCP-NSALINES1X, FluxMPS™ Normal Saline (Sodium Chloride), 0.9% (w/v), Sterile.

Supporting literature

Curated references relevant to isotonic saline solutions, microphysiological systems, and cell culture handling.

1. Huh D, et al. Reconstituting Organ-Level Lung Functions on a Chip. Science, 2010. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol, 2014. doi:10.1038/nbt.2989
3. Wikswo JP. The relevance and potential roles of microphysiological systems in biology and medicine. Exp Biol Med, 2014. doi:10.1177/1535370214542068
4. Low LA, et al. Organs-on-chips: into the next decade. Nat Rev Drug Discov, 2021. doi:10.1038/s41573-020-0079-3
5. Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. Wiley, 2015. doi:10.1002/9781118873686
6. Lanigan RS, Yamarik TA. Final report on the safety assessment of sodium chloride. Int J Toxicol, 2001. doi:10.1080/109158101750300937
7. Awad S, et al. The history of 0.9% saline. Clin Nutr, 2008. doi:10.1016/j.clnu.2008.01.008
8. Rasmussen KM, et al. Isotonic fluids and cell viability in tissue culture applications. J Vis Exp, 2016. doi:10.3791/54317
9. Uphoff CC, Drexler HG. Detection of mycoplasma contamination in cell cultures. Curr Protoc Mol Biol, 2014. doi:10.1002/0471142727.mb2404s106
10. United States Pharmacopeia. General Chapter <1231> Water for Pharmaceutical Purposes. USP-NF, 2023. doi:10.31003/USP-NF_M99860