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FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o Folic Acid: 1X Liquid

Product#: DCP-DMEMWOFA1X
$44.00
DCP-DMEMWOFA1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o Folic Acid: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose (High, 4500 mg/L) Contains Sodium Pyruvate Without Folic Acid

FluxMPS™ DCP-DMEMWOFA1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) DMEM High Glucose — Folic Acid–Free formulation engineered for DHFR-based selection and gene amplification research. It carries the same high-glucose, L-glutamine, sodium pyruvate and sodium bicarbonate formulation as standard DMEM High Glucose, with folic acid intentionally omitted. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Folic acid–free formulation — creates the selective pressure required for DHFR-mediated gene amplification in CHO-DHFR-deficient cell lines
  • Compatible with methotrexate (MTX) step-selection protocols for recombinant protein expression amplification
  • All other High Glucose DMEM nutrients intact: 4500 mg/L D-glucose, 584 mg/L L-glutamine, 110 mg/L sodium pyruvate, 3700 mg/L sodium bicarbonate
  • Quadruple-stage filtration (0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish)
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Sodium bicarbonate-buffered (3700 mg/L); formulated for a 10% CO₂ incubator atmosphere at pH 7.4
  • Manufactured under an ISO 13485:2016 quality management system with per-lot Certificate of Analysis
  • Custom formulation modifications available on request — pH, salts, and nutrient adjustments
CAT. NO.
DCP-DMEMWOFA1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o Folic Acid: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • Key featureFolic Acid–Free
  • Formulation[+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (High), [+] Sodium Pyruvate, [-] Folic Acid
  • AppearanceRed-colored (phenol red indicator), clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (4 stages)
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered for DHFR selection and folate metabolism research

Standard DMEM High Glucose contains folic acid — a substrate cells can use to bypass dihydrofolate reductase (DHFR) dependency. Removing folic acid creates the selective pressure needed for DHFR-mediated gene amplification, methotrexate step selection, and any study where folate pathway activity must be controlled. FluxMPS™ delivers this folic acid–free formulation through the full quadruple-stage filtration train, with a < 0.05 EU/mL endotoxin release specification, for use in CHO cell lines and microfluidic bioproduction models.

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Microchannel-safe purity

0.04 µm final filtration and USP <788> Method 1 particulate compliance support safe perfusion in narrow chip geometries, including sub-100 µm channels.

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Precise formulation control

Folic Acid–Free — a defined omission that enables DHFR selection, MTX step-selection, and folate pathway research requiring exact control of this single component.

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Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696) minimizes trace-metal and organic-carbon background that could interfere with sensitive assays.

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Low endotoxin release specification

< 0.05 EU/mL endotoxin release specification, tested per batch by LAL assay, helps reduce the risk of LPS-driven inflammatory signaling confounding sensitive culture readouts.

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High-glucose energy support

4.5 g/L D-glucose sustains the energy demands of CHO bioproduction and other high-metabolic-rate protocols targeted by this formulation.

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Customization on demand

pH, glucose, salts, and additional component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris and protein aggregates; protects the first 0.04 µm cartridge from fouling.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains mycoplasma (0.2–0.3 µm) and sub-micron particulates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge — not a polish of Stage 2 effluent, but redundant protection for the final filter.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
4
Sequential filtration passes to a 0.04 µm final cut-off
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma is controlled by 0.1 µm mycoplasma-retentive filtration at every production stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEMWOFA1X Dulbecco’s Modified Eagle Medium (DMEM), High Glucose w/o Folic Acid: 1X Liquid ? Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II Polish ? Microfluidics Suitable DMEM High Glucose Folic Acid-Free for organ-on-a-chip and microfluidic applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) used to process DCP-DMEMWOFA1X.
© Diagnocine® — DCP-DMEMWOFA1X
Applications

Specialized applications requiring Folic Acid–Free medium

FluxMPS™ DCP-DMEMWOFA1X is designed for research protocols where folic acid–free conditions are essential — combined with Microfluidics Suitable filtration purity for organ-on-a-chip and microfluidic platform compatibility.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available for automated bioreactor perfusion and robotic liquid handlers — a separate six-stage process beyond the Microfluidics Suitable line described on this page.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion circuits
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

hub

Organ-on-a-Chip & Microphysiological Systems (MPS)

Quadruple-stage 0.04 µm filtration helps prevent microchannel clogging. Folic Acid–Free formulation is Microfluidics Suitable for specialized OoC protocols requiring folate-pathway control.

OoCMPSToCBoC
science

CHO Bioproduction

FluxMPS™ DCP-DMEMWOFA1X delivers Folic Acid–Free conditions with Microfluidics Suitable filtration purity for demanding CHO bioproduction platforms.

analytics

DHFR Gene Amplification

Folic Acid–Free conditions support DHFR-mediated gene amplification workflows in CHO-DHFR-deficient cell lines.

biotech

Methotrexate Selection

Compatible with stepwise MTX selection protocols used to drive transgene copy number and expression level.

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Recombinant Protein Expression

Supports recombinant protein expression platforms that rely on DHFR/MTX amplification for high-titer production.

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Folate Metabolism Research

A defined folic acid–free background for studies of folate pathway activity, one-carbon metabolism and related signaling.

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix below. Available pack sizes: 500 mL, 1000 mL. Certificate of Analysis: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Glucose (High), [+] Sodium Pyruvate, [-] Folic Acid
Appearance Red-colored (phenol red indicator), clear solution
Glucose 4500 mg/L (4.5 g/L, High Glucose)
Key feature Folic Acid–Free
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H2O
Total ingredients 32 components across 3 tabbed categories
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release spec.)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100) aseptic fill
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement 10% CO₂ incubator atmosphere (derived from 3700 mg/L sodium bicarbonate buffering at pH 7.4)
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

DMEM High Glucose — Folic Acid–Free: 32 ingredients verified per lot with CAS numbers for full raw-material traceability. Folic acid is intentionally absent from this formulation; do not add folic acid unless ending the selection period. All other vitamins, amino acids and inorganic salts are present at standard DMEM High Glucose concentrations.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Glutamine 56-85-9 584.000
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
i-Inositol 87-89-8 7.200
OTHERS
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 15.900
Sodium pyruvate 113-24-6 110.000
Custom formulation: Contact support@diagnocine.com for DCP-DMEMWOFA1X modifications — component additions, pH changes, or nutrient adjustments.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

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ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm feed water, minimizing trace-metal and organic-carbon background.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

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Micro-Batch Precision

Small-batch, per-lot tested — no blending; a Certificate of Analysis is issued for every lot.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 310–350 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMWOFA1X compares

FluxMPS™ DCP-DMEMWOFA1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Parameter DCP-DMEMWOFA1X (FluxMPS™) Conventional DMEM HG
(0.22 µm filtered)		 Standard DMEM HG
(0.22 µm filtered)
Grade Microfluidics Suitable Not applicable Not applicable
High Glucose DMEM without Folic Acid — DHFR selection, gene amplification, folate metabolism studies check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMWOFA1X — DMEM High Glucose Folic Acid–Free.

Yes. DCP-DMEMWOFA1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low particulate levels suitable for MPS, OoC, ToC and BoC platforms. The Folic Acid–Free formulation carries the same < 0.05 EU/mL endotoxin release specification as the rest of the FluxMPS™ line.
 
Folic acid is removed because cells expressing the DHFR (dihydrofolate reductase) transgene can synthesize their own tetrahydrofolate from dihydrofolate — but only if exogenous folic acid is absent. In folic acid–free medium, only cells that have successfully integrated and are expressing DHFR survive. Combined with stepwise methotrexate (MTX) treatment, increasing MTX concentrations drive gene amplification to produce more DHFR (and the co-amplified transgene of interest), increasing recombinant protein expression. This is the basis of CHO-DHFR-deficient selection systems used in biopharmaceutical manufacturing. All other formulation components (glucose, glutamine, pyruvate, bicarbonate) remain at standard DMEM High Glucose concentrations — see the Full Composition table.
Yes. This formulation is buffered with 3700 mg/L sodium bicarbonate, which requires an approximately 10% CO₂ incubator atmosphere to maintain the stated pH of 7.4 (Henderson-Hasselbalch relationship). Verify the CO₂ setpoint for your specific incubator against this bicarbonate concentration before use.
Yes. Add FBS (typically 5–10%), serum-free supplements, growth factors, antibiotics, or custom nutrients as required by your protocol. When supplementing with serum or other protein-containing additives, filter through a 0.2 µm low-protein-binding PES or PVDF membrane — never 0.04 µm, which retains serum proteins and lipoproteins and will clog rapidly. Contact support@diagnocine.com for custom co-formulation.
FluxMPS™ DCP-DMEMWOFA1X is produced to meet a release specification of < 0.05 EU/mL by LAL assay (USP <85>). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification, with a Certificate of Analysis available on request.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting the research applications of FluxMPS™ DCP-DMEMWOFA1X.

  1. Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216–4220. doi:10.1073/pnas.77.7.4216
  2. Kaufman RJ, Sharp PA. Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary DNA gene. J Mol Biol. 1982;159:601–621. doi:10.1016/0022-2836(82)90103-6
  3. Wurm FM. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol. 2004;22:1393–1398. doi:10.1038/nbt1026
  4. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  5. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  6. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  7. Locasale JW. Serine, glycine and the one-carbon cycle: cancer metabolism in full circle. Nat Rev Cancer. 2013;13:572–583. doi:10.1038/nrc3557
  8. Ducker GS, Rabinowitz JD. One-carbon metabolism in health and disease. Cell Metab. 2017;25:27–42. doi:10.1016/j.cmet.2016.08.009
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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