Dulbecco's PBS without Ca2+and Mg2+ (D-PBS), Sterile

FluxMPS™ Dulbecco's PBS without Ca²⁺ and Mg²⁺ (D-PBS), Sterile

An MPS-grade Dulbecco's phosphate buffered saline formulated to pH 7.4 without calcium or magnesium, prepared in ASTM Type 1 ultrapure deionized water and sterile-filtered through a 0.1 µm membrane for microchannel-safe use in organ-on-a-chip, microfluidic, and general cell culture workflows. Free of calcium, magnesium, protease, DNase, RNase, and endonuclease activity, and manufactured under ISO 13485-certified, CE-approved facilities.

• Formulated to pH 7.4 without calcium or magnesium for gentle, isotonic cell washing and dissociation prep
• Sterile-filtered through a 0.1 µm membrane, applied twice (2X), in a controlled clean environment
• Prepared with ASTM 0.05 µS/cm Type 1 Ultrapure water, deionized
• Protease-, DNase-, RNase-, and endonuclease-negative; calcium- and magnesium-free
• Tissue Culture and Molecular Biology grade, manufactured under ISO 13485-certified, CE-approved facilities
• Supplied as a clear, colorless, ready-to-use sterile liquid
• Custom pH, concentration (2X, 4X, 5X, 10X), and additive formulations available on request

Engineered where standard buffers fail

Conventional 0.22 µm-filtered buffers can carry subvisible particulates that accumulate in microchannels, drift in pH or ionic composition during storage, and introduce background into sensitive assays. FluxMPS™ buffers are built around a defined, lot-traceable formulation and a validated filtration process to reduce these risks.

Single-stage 0.1 µm filtration, applied twice

This buffer is sterile-filtered through a 0.1 µm membrane in a controlled clean environment, with the pass repeated twice (2X) to reinforce sterility assurance before fill.

1. 1

   0.1 µm Sterile Filtration

   All reagents are passed through a 0.1 µm membrane filter, applied twice (2X), in a controlled clean environment. This largely prevents mycoplasma contamination, since the smallest mycoplasma organisms are approximately 0.2 micron — above the filter's rated pore size.

Where D-PBS without Ca²⁺/Mg²⁺ is used

Dulbecco's phosphate-buffered saline is a balanced salt solution with osmotic balance similar to mammalian systems, used to sustain the physiological and structural integrity of cells outside their regular growth environment. Without calcium and magnesium, it protects cells from acute fluctuations and supports more effective trypsinization and dissociation. Common uses include general cell culture, washing adhesion cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents that supply inorganic salts for basic cell metabolism.

Automated Bioreactors & Robotics

For automated perfusion systems and robotics-driven workflows, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available on request to further protect sensitive valves, sensors, and long-duration perfusion loops.

• Total Particulate Exclusion for automated fluidic pathways
• Valve & Sensor Protection in closed-loop perfusion systems
• Extended Perfusion Stability for long-duration culture runs

Inquiry Required: the 0.01 µm ultra-filtered grade is a custom order — contact support@diagnocine.com to request this variant.

Physical, chemical & quality parameters

Specification values below reflect only data stated for this product; parameters not disclosed in the source documentation are omitted rather than estimated.

Full composition

All component concentrations are shown as stated for this product, with CAS numbers added where known.

Manufacturing & compliance

This buffer is manufactured under ISO 13485-certified, CE-approved facilities, with final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.

How DCP-DPBSWOCAMG1X compares

A qualitative comparison against conventional single-pass 0.22 µm-filtered buffer and a standard alternative.

Frequently asked questions

Answers below reflect only the specifications published for this product.

Supporting literature

General references relevant to D-PBS use in cell culture and organ-on-a-chip/microfluidic systems.

1. Dulbecco R, Vogt M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954;99(2):167-182. doi:10.1084/jem.99.2.167
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760-772. doi:10.1038/nbt.2989
3. Huh D, Matthews BD, Mammoto A, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662-1668. doi:10.1126/science.1188302
4. Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345-361. doi:10.1038/s41573-020-0079-3
5. Ingber DE. Human organs-on-chips for disease modeling, drug development and personalized medicine. Nat Rev Genet. 2022;23:467-491. doi:10.1038/s41576-022-00466-9
6. Sung JH, Wang YI, Narasimhan Sriram N, et al. Recent advances in body-on-a-chip systems. Anal Chem. 2019;91(1):330-351. doi:10.1021/acs.analchem.8b05293
7. Zhang B, Radisic M. Organ-on-a-chip devices advance to market. Lab Chip. 2017;17(14):2395-2420. doi:10.1039/C6LC01554A
8. van Meer BJ, de Vries H, Firth KSA, et al. Small molecule absorption by PDMS in the context of drug response bioassays. Biochem Biophys Res Commun. 2017;482(2):323-328. doi:10.1016/j.bbrc.2016.11.062
9. Low LA, Tagle DA. Tissue chips - innovative tools for drug development and disease modeling. Lab Chip. 2017;17(18):3026-3036. doi:10.1039/C7LC00462A