Magnesium Assay kit LS - Xylidyl Blue-I method

Product#: MG01ME
$310.00
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Magnesium Assay kit LS - Xylidyl Blue-I method

Cat. No. MG01ME
Size 200 tests / 1 kit
Store oC
Measuring range  0.2 - 5.0 mg/dl
Wavelength (maximume wavelength) 660 nm
Chromogen Xylidyl Blue-I
Target Copper Magnesium(Mg)


Description

Metallo Assay Magnesium Assay kit LS is intended for the quantitative determination of magnesium in biological sample by 96-well reader use. Target: magnesium(Mg) Measuring range: 0.2-5.0 mg/Dl Species: all. Sample: serum, plasma, urine, saliva, tissue extract, cell lysate, biological fluid, food, beverage etc. Apparatus: UV/Vis 96-well reader or spectrophotometer Wavelength: 660 nm Procedure: 10 min

Now, the trace metals can be measured easily by microplate reader!
"Metallo Assay LS" Series

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AlI ATP-dependent enzymes require Mg2+ as a cofactor to reacts enzyme and maintains the electrical excitability of the muscular and nervous cells. Regulation takes place mainly via the kidneys, especially via the ascending loop of Henle. A low magnesium level is found in malabsorption syndrome, diuretic or aminoglucoside therapy. Hypermagnesemia is found in acute and chronic renal failure, glomerulonephritis, Addisons’s disease or intensive anti acid therapy, magnesium excess, and magnesium release from the intracellular space.

This product is a direct colorimetric assay kit without deproteinization of the sample. Magnesium with Xylidyl blue-I (as chelator) at alkaline pH, yields a purple colored complex. The intensity of the colour formed is proportional to the magnesium concentration in the sample.

Notes
  • Unstableness of incubation temperature may result in unstable results.
  • Use disposable test tube and glassware washed with 1M HNO3 or 1M HCl solution and distilled water.
  • Accuracy in pipetting volume for samples and reagents may affect the quality of assay. Please note that samples, standards and Working Reagent must be poured accurately µL level.
  • Temperature for chromogen reaction may affect optical density. Please try to extend or shorten chromogen reaction time depending on room temperature.
  • In the cell lysate or the tissue extract use as specimen, high concentration of proteins or lipid, may affect observed value. Please remove its by ultrafiltration or centrifugation.
Expiration date and preservation conditions
Storage conditions: Store at 2-8°C. Don’t freeze.
Expiration: 1 year from the date of manufacture. After the bottles are opened, the kit should be used in 1 month.

Operation
1. Sample preparation
Serum or Plasma
Insoluble substances in serum and plasma samples should be
removed by filtration or centrifugation. EDTA-plasma cannot
be used.
Tissue extract, Lysate, Other samples.
Urine (24 hour pooled urine), or other biological fluid:
Add 6M HCl to the sample and adjust pH 2.0-3.0 (e.g. 5-10µL
6M HCl/ 1mL of lysate.). Centrifuge at 6,000 rpm for 15 min.
Collect the supernatant and use it for assay.
Tissue:
Add 5% TCA solution, vortex 1 min. and incubate at 4-8°C for
30 min. Centrifuge at 6,000 rpm for 15 min. Collect the
supernatant and use it for assay.
* Sample pH should be between pH2 to pH8.

2. Assay preparation
Bring all reagents to room temperature before use.

3. Assay procedure.
Procedure using microplate reader. (1 assay sample 253 µL) OAssay
(1) Add 3 µL of Distilled water (Blank) / STD (Standard)/ sample into each well.
(2) Add 250 µL of R-R to each well and incubate at room temperature for 5 min.
(3) Read the absorbance at 660 nm. --> OD

Assay Result
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Example of use
  • Metallomics research
  • Trace metal research as effector of enzyme / transcription activity
  • Quantification of heavy metal accumulated in body
  • Quantification of trace metal for drag discovery support research
  • Research of relation between plant growth factor and trace metal
  • Research for metabolism / kinetics mechanism of trace metal in the cell
  • Trace metal research as trigger of reactive oxygen species (ROS)
  • Research of relation between trace metal metabolism / dynamic derangement and disease
  • Research of trace metal as a cofactor of antioxidant enzyme
  • ...and more!
Reference
  1. Mann C. K, Yoe J. H : Spectrophotometric determination of magnesium with sodium 1-azo-2-haydroxy-3-(2,4-dimethyl -carboxanili-do)-naphtalene-1-(2-hydroxy-benzene-5-sulph onate), Anal Chem, 28, p202-205 (1956)
  2. Sakamoto. A, Terui. Y, Yamamoto. T, Kasahara. T, Nakamura. M, Tomitori. H, Yamamoto. K, Ishihama A, Michael. AJ, Igarashi. K, Kashiwagi. K : Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor, Int J Biochem Cell Biol, 44(15), p1877-1886 (2012).  

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