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Human Matrix metalloproteinase 1,MMP-1 ELISA kit
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Human Matrix metalloproteinase 1,MMP-1 ELISA kit
Sandwich quantitative immunoassay for matrix metallopeptidase 1 (interstitial collagenase) in human serum, plasma, cell culture supernates, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | matrix metallopeptidase 1 (interstitial collagenase) |
| Uniprot No. | P03956 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection range | 0.312 ng/mL-20 ng/mL |
| Sensitivity | 0.078 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cancer |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, matrix metallopeptidase 1 (interstitial collagenase) in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 0.312 ng/mL-20 ng/mL with a minimum detectable dose of 0.078 ng/mL.
This Human MMP1 ELISA Kit was designed for the quantitative measurement of Human MMP1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .
- Review/Meta-analysis: MMP-1 -1607 1G/2G and MMP-12 -82 A/G gene polymorphisms are associated with ischemic stroke risk. PMID: 28958661
- There was no statistically significant difference in the distribution of the MMP1-1607dupG (rs1799750) polymorphism between patients with unstable angina and the control group. PMID: 29044936
- MMP-1 (and MMP-2 and MMP-3) are independently associated with markers of arterial stiffening in patients with type 1 diabetes. PMID: 29070037
- Matrix metalloproteinase 1 rs1799750 polymorphism was significantly associated with nasopharyngeal carcinoma risk in the subgroup analysis by smoking, a marginally significant association was found in non-smokers. PMID: 29096757
- The results of the present study suggested that miR93 regulated MMP1 and collagen I expression in fibroblasts via calpain2. miR93 mediated collagen expression in stress urinary incontinence via calpain2. PMID: 29115452
- Specific silencing that targeted AMPK resulted in the enhancement of mobility and contractility and in the production of MMP-1 and MMP-2. PMID: 29122080
- Results suggested that miR-361 directly inhibited MMP-1 expression by binding to its 3'-UTR suppressing breast cancer cells invasion and metastasis. PMID: 29132384
- Authors observed a significant increase in the immunoexpression of collagen I and III in patients with DCM and a significant reduction in the immunoexpression of MMP-1 compared with the control group. Also, the collagen I and TIMP-1 expression indicated a positive linear correlation and respectively a negative linear relationship with collagen III and MMP-1. PMID: 29250654
- Reduced expression of types I and III collagen and TIMP-1 as well as the increased expression of MMP-1 and MMP-8 in the anterior vaginal wall tissues play important roles in the onset of pelvic organ prolapse. PMID: 29263043
- RHCG Suppresses Tumorigenicity and Metastasis in Esophageal Squamous Cell Carcinoma via Inhibiting NF-kappaB Signaling and MMP1 Expression. PMID: 29290801
- No significant association was found between MMP-1 (-16071G/2G, -519A/G) variants and obesity. PMID: 29317790
- MMP-1 genotype may accelerate the development of HIV-associated neurocognitive disorder (HAND) whereas MMP3 -1612 5A5A genotype may reduce the risk of pathogenesis of HAND. PMID: 29358561
- Expression of GSDMC is increased by UV irradiation in human skin keratinocytes. GSDMC induction contributes to the increase of MMP-1 expression specifically via activation of ERK and JNK pathways. PMID: 29428815
- The current study indicated that MMP1 has a key role in the regulation of cervical tumor growth and lymph node metastasis via epithelial mesenchymal transition to a certain extent PMID: 29436615
- rs1799750 in MMP1 might be a risk factor for rheumatic heart disease in a Han population in Southern China, and individuals carrying the 2G/2G genotype are likely more susceptible to rheumatic heart disease. PMID: 29458338
- this study provides insights into the development of a possible alternative therapy manipulating MMP-1 and the mTOR signaling pathway in Epidermal growth factor receptor-tyrosine kinase inhibitor-resistant lung adenocarcinoma. PMID: 29463039
- The maternal and fetal 1G/1G MMP1 gene polymorphisms may be strong genetic markers of preeclampsia, occurring either individually or together. PMID: 29670668
- our results suggest that the typical 1G/2G MMP-1 polymorphic site did not play a direct determinant role in breast cancer among Taiwanese women. PMID: 29695550
- 2G allele of MMP-1, C allele of MMP-2 and 5A/6A genotype of MMP-3 are associated with susceptibility and disease progression of type 2 diabetic nephropathy PMID: 29883760
- findings suggest that the polymorphic genotypes at MMP-1 promoter -1607 may play a major role in determining personal cancer susceptibility for prostate cancer in Taiwan PMID: 29970511
- the present study demonstrated that scopoletin inhibited MMP1 and proinflammatory cytokine expression by inhibiting p38 MAPK phosphorylation. PMID: 30015831
- For MMP-1(-1607) 1G/2G, a significant association was observed using the recessive genetic model. In the subgroup studies, a significant association was observed in the Asian subgroup (OR = 1.68; for the recessive model; and OR = 1.59; for the allelic contrast model) and in the European subgroup (OR = 0.65; for the allelic contrast model). PMID: 30117779
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 93-105 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 95-108 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| The recovery of human MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MMP-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled MMP-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
