qFlamma® Black01 Biotin

qFlamma® Black01 Biotin is a versatile dark quencher designed for use in various biochemical and biological research applications. This universal quencher exhibits a broad absorption range from 400 to 800 nm, with a maximum absorption at 490 nm, and functions without native fluorescence13. The Black01 quencher component demonstrates exceptional properties, including a high absorption coefficient, remarkable stability, and excellent aqueous solubility.

The biotin moiety of qFlamma® Black01 Biotin enables strong and specific binding to avidin and streptavidin, making it an ideal choice for numerous research applications1. This green solid compound has a molecular weight of 962.08 g/mol and is soluble in water, DMF, and DMSO3. For optimal performance and longevity, it should be stored at -20°C and protected from light.

Applications
1. Affinity chromatography
2. Polymerase Chain Reaction (PCR)
3. In situ nucleic acid hybridization
4. Multiplexed bio-analysis
5. Fluorescence Resonance Energy Transfer (FRET) probes
6. Protease activity assays

Advantages
1. Broad absorption range: Effectively quenches a wide variety of commercially available fluorescent dyes
2. High absorption coefficient: Ensures efficient quenching of fluorescence
3. Superb stability: Maintains performance under various experimental conditions
4. Excellent aqueous solubility: Facilitates easy manipulation in aqueous buffers
5. No native fluorescence: Eliminates background fluorescence, improving signal-to-noise ratio
6. Biotin-Avidin/Streptavidin binding: Provides high affinity and specificity for diverse applications
7. Versatility: Can be used in single-tube multiplexing assay systems due to its spectral properties
8. Compatibility: Works well with most biomolecules

These features make qFlamma® Black01 Biotin an excellent choice for researchers seeking a reliable and efficient dark quencher for their experiments in biochemical and biological fields.

 

Specifications

• Functionality: Dark quencher
• Reactive group: Biotin
• Absorption max. (nm): 490
• Absorption range (nm): 400 ~ 800
• Appearance: Green Solid
• Molecular Weight: 962.08 g/mol
• Solubility: Water, DMF, DMSO
• Storage conditions: -20 ℃, protect from light

 qFlamma® Quenchers
 

Quick link (Cat.#)	Series	Quick link (Cat.#)	Series
QWS1001	qFlamma® Black01 NHS ester	QWSN1001	qFlamma® Black01 Sulfo-NHS ester
QWA1001	qFlamma® Black01 Vinylsulfone	QWC1001	qFlamma® Black01 Carboxylic acid
QWM1001	qFlamma® Black01 Maleimide	QWE1001	qFlamma® Black01 Amine
QWT1001	qFlamma® Black01 Thiol	QWR1001	qFlamma® Black01 Dichlorotriazine
QWG1001	qFlamma® Black01 PEG4-Alkyne	QWD1001	qFlamma® Black01 ADIBO
QWB1001	qFlamma® Black01 Biotin	QWK1001	qFlamma® Black01 Alkyne
QWZ1001	qFlamma® Black01 Azide	QWH1001	qFlamma® Black01 Hydrazide

qFlamma® Quenchers

Dark quenchers are substances that absorb excitation energy from a fluorophore and dissipate the energy as heat without any native fluorescence. The combination of a fluorescent dye and quencher may act as an on/off switch of fluorescence that the original fluorescence might be restored as the distance between them increases or when their connection is cleaved. In conjunction with fluorophores, dark quenchers are regarded as key components in designing FRET probes, in protease activity assays, nucleic acid hybridization, PCR, etc. The main advantage of dark quencher is the non-emissive excitation and elimination of background fluorescence. BioActs developed a new generation of azo-anthraquinone derivative dark quenchers, qFlamma® quenchers, to overcome issues associated with existing dark quenchers such as stability, quenching ability and water solubility.

The absorbance peaks of qFlamma® quenchers completely cover spectral range from visible to near-infrared (NIR) that all of commercially available fluorescent dyes can be effectively quenched. The quenchers display high absorption coefficient together with excellent quenching ability, superb stability and aqueous solubility so that it can easily manipulated in aqueous buffers. The four of qFlamma® quenchers (Black 01, Red, Blue and Orange) series cover the electromagnetic spectrum from 400 nm to NIR region and provides excellent spectral overlap across the entire range of known fluorescent dyes. The complete spectral overlap of qFlamma® quencher series along with their variety of reacting groups enable to design single-tube multiplexing assay system owing their capability for efficient quenching and the preventing cross-talking between dyes. BioActs offers qFlamma® quenchers as universal dark quenchers for the construction of a variety of hybridization and custom-synthesized probes incorporated spectrally paired fluorophores and quenchers for multiplexed bio-analysis, PCR, nucleic acid hybridization, and high-throughput imaging assays.