qFlamma® Black01 Sulfo-NHS ester

qFlamma® Black01 Sulfo-NHS ester is an amine reactive form of universal dark quencher that the range of absorption covers from 400 to 800 nm with no native fluorescence. Black01 quencher displays high absorption coefficient, superb stability and aqueous solubility. Sulfo-NHS esters have higher water solubility than NHS esters, thus they do not need organic co-solvent and readily react with amino groups of probes, peptides or the amine terminus of nucleotides to form a chemically stable amide bond between the quencher and the biomolecule. We offer qFlamma® Black01 Sulfo-NHS ester as a universal quencher in the construction of a variety of hybridization and custom-synthesized activatable probes for multiplexed bio-analysis, PCR, nucleic acid hybridization, etc.  
 

Specifications

• Functionality: Dark quencher
• Reactive group: Sulfo-NHS ester
• Absorption max. (nm): 490
• Absorption range (nm): 400 ~ 800
• Extinction coefficient: 21,000 cm-1M-1
• CF280: 0.8
• Appearance: Green Solid
• Molecular Weight: 818.83 g/mol
• Solubility: Water, DMF, DMSO
• Storage conditions: -20 ℃, protect from light

 qFlamma® Quenchers
 

Quick link (Cat.#)	Series	Quick link (Cat.#)	Series
QWS1001	qFlamma® Black01 NHS ester	QWSN1001	qFlamma® Black01 Sulfo-NHS ester
QWA1001	qFlamma® Black01 Vinylsulfone	QWC1001	qFlamma® Black01 Carboxylic acid
QWM1001	qFlamma® Black01 Maleimide	QWE1001	qFlamma® Black01 Amine
QWT1001	qFlamma® Black01 Thiol	QWR1001	qFlamma® Black01 Dichlorotriazine
QWG1001	qFlamma® Black01 PEG4-Alkyne	QWD1001	qFlamma® Black01 ADIBO
QWB1001	qFlamma® Black01 Biotin	QWK1001	qFlamma® Black01 Alkyne
QWZ1001	qFlamma® Black01 Azide	QWH1001	qFlamma® Black01 Hydrazide

qFlamma® Quenchers

Dark quenchers are substances that absorb excitation energy from a fluorophore and dissipate the energy as heat without any native fluorescence. The combination of a fluorescent dye and quencher may act as an on/off switch of fluorescence that the original fluorescence might be restored as the distance between them increases or when their connection is cleaved. In conjunction with fluorophores, dark quenchers are regarded as key components in designing FRET probes, in protease activity assays, nucleic acid hybridization, PCR, etc. The main advantage of dark quencher is the non-emissive excitation and elimination of background fluorescence. BioActs developed a new generation of azo-anthraquinone derivative dark quenchers, qFlamma® quenchers, to overcome issues associated with existing dark quenchers such as stability, quenching ability and water solubility.

The absorbance peaks of qFlamma® quenchers completely cover spectral range from visible to near-infrared (NIR) that all of commercially available fluorescent dyes can be effectively quenched. The quenchers display high absorption coefficient together with excellent quenching ability, superb stability and aqueous solubility so that it can easily manipulated in aqueous buffers. The four of qFlamma® quenchers (Black 01, Red, Blue and Orange) series cover the electromagnetic spectrum from 400 nm to NIR region and provides excellent spectral overlap across the entire range of known fluorescent dyes. The complete spectral overlap of qFlamma® quencher series along with their variety of reacting groups enable to design single-tube multiplexing assay system owing their capability for efficient quenching and the preventing cross-talking between dyes. BioActs offers qFlamma® quenchers as universal dark quenchers for the construction of a variety of hybridization and custom-synthesized probes incorporated spectrally paired fluorophores and quenchers for multiplexed bio-analysis, PCR, nucleic acid hybridization, and high-throughput imaging assays.