iMatrix-411 (6x175 µg)

Product#: 892042
$1,200.00
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iMatrix 411 (6 X 175ug)

 

Cat. No.:   892042
Size:   6 X 175ug
Description:   iMatrix-411 is a recombinant human laminin-411 E8 fragment protein expressed in Chinese Hamster Ovary (CHO)-S cells. iMatrix-411 contains the integrin-binding site of the laminin-411 molecule. iMatrix-411 is a useful cell culture substrate for proliferation and differentiation of vascular endothelial cells and bile duct epithelial cells. iMatrix411 is also useful for the culture of other cells adhering to laminin-411.
Content:   Recombinant human laminin-411 E8 fragment protein in PBS(-)
Concentration:   0.5 mg/mL
Amount:   175 μg / 0.35 mL / tube  (1050 μg / 6 tubes)
Storage:   Store at 2°C to 15°C, protect from light.
Expiration date:   The shelf life is 2 years from the date of manufacture. The expiration date is printed on the outer carton.
Activity:   The dissociation constant (Kd) for the binding with integrin α6β1 is 10 nM or less.
Methods of use:   By the following method, iMatrix-411 can be coated onto a culture vessel. The optimum coating density may differ by cell-type, cell-line, medium selected, or purpose. Insufficient coating density may result in the detachment of cells and varied cell conditions while the excessive coating density may lead to difficulty in detaching cells for passage.

Determine the optimal coating density. 0.5 μg/cm2 is a standard but test between 0.1 and 1.5 μg/cm2.
  1. Dilute iMatrix-411 with PBS(-). Use the diluted iMatrix-411 immediately. To coat with 0.5 μg/cm2 onto a 6-well plate with 9.6 cm2/well, dilute 9.6 μL of iMatrix-411 with 2 mL of PBS(-) per well.
  2. Place the diluted iMatrix-411 into a culture vessel and incubate either at 37°C for 1 h, or at room temperature for 3 h, or at 4°C overnight.
  3. Aspirate the coating solution. Then, immediately seed your cells. Do not allow the coated surface to dry.
*If you face difficulties in detaching cells for passage, re-adjust the conditions (e.g., reduce the coating density).
References:  
  1. Nishiuchi R. et al. (2006), Matrix Biol. 25 (3): 189-97
  2. Ohta R. et al. (2016), Sci. Rep. 6: 35680
  3. Takayama K. et al. (2016), Biochem. Biophys. Res. Commun. 474 (1): 91-6
  4. Tang J. and Saito T. (2018), BioMed. Res. Int. 2018: 9465383
Caution:   For research use only. Not intended for human use. In the event of accidental ingestion or contact with the eyes, immediately wash the affected area and seek medical attention.


The Biology behind the Technology

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Laminins are an essential component of the extracellular matrix and make up a large portion of the basement membrane. They support and regulate cell growth, motility and differentiation.  Laminins are trimeric proteins made up of a, /3 and y chains and the nomenclature of the different isoforms is based on the type of chain that it contains. 

Of note, lamimin 511 is expressed during embryogenesis and research by Professor Kiyotoshi Sekiguchi at Osaka University in Japan has unveiled that the cellular adhesion site is located in what is named the E8 region. Through his work, it was also found that the laminin E8 fragment provided superior performance as a substrate compared to full-length laminin. 



Laminins are cell adhesion proteins

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Combination of laminins and cell type in vivo


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A series of recombinant human laminins as various cell culture substrate.

Laminin is one of the extra cellular matrixes (ECM) located in the basement membrane of animals, and play an important role for cell adhesion and proliferation. Laminin is composed of three parts ; alpha, beta, and gamma chain, and 15 types of laminin has been already known.

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Induce endothelial cells from pluripotent stem cells


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Phenotypes of endothelial cells induced from pluripotent stem cells on iMatrix-411 coated plate.
Acetyl-LDL uptake (Red), CD31 (Green) expression and nuclear stainiing (Blue).
Scale bar : 10 μm




iMatrix™ Cell Culture Substrates

Product Name Product ID Description Usage Size Link
imatrix221_F0.png iMatrix-221 892061 Recombinant Human Laminin-211
E8 Fragments
Cardiomyocytes 2 × 175 μg Go_Button
892062 6 × 175 μg Go_Button
iMatrix411_F1.png iMatrix-411 892041 Recombinant Human Laminin-411
E8 Fragments
Endothelial cells 2 × 175 μg Go_Button
892042 6 × 175 μg Go_Button
iMatrix511_F1.png iMatrix-511 892011 Recombinant Human Laminin-511
E8 Fragments
Pluripotent Stem Cells 2 × 175 μg Go_Button
892012 6 × 175 μg Go_Button
iMatrix_511_Silk_F1.png iMatrix-511 Silk 892021 Recombinant Human Laminin-511
E8 Fragments, expressed in Silkworm
Pluripotent Stem Cells 6 × 175 μg Go_Button


 

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