iMatrix-511 Silk (0.5 mg/ml solution) (6x175 ug)

Description

iMatrix-511 (Laminin-511 E8) is beneficial for your stem cell culture!

iMatrix-511, is the recombinant human Laminin-511 E8 fragment, produced by CHO-S cells and it can reduce the risk of the xeno-components contamination in the culture. Handling is very easy - just dilute in PBS(-), coat it in the culture dish or plate and incubate at room temparature for 3 hours or at 4°C over night.

Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals, and play an important role for cell adhesion and proliferation.

Laminin is composed of three parts ; alpha, beta, and gamma - chain and 15 types of laminin is already known. Among them, Laminin composed by Alpha5-chain, Beta1-chain, and Gamma1-chain, is called "Laminin-511".
Laminin-511 is recognized by alpha6beta1 integrin. Cell adhision, extension and proliferation is caused by many cell signalings through intergrin.

Laminin-511 E8 is the fragment of Laminin-511 obtained by enzyme digestion.
Although this is fragment, it has binding ability to alpha6beta1 integrin.
According to Miyazaki's report ( Nat. Commun. 3, 1236 (2012)), Laminin-511 E8 could be used for cell culture of ES cells and iPS cells.


To date, mouse feeder cells or matrigel have been used for the culture of iPS cells and ES cells.
However, these cell culture substrates have a risk to bring xeno-components in the culture condition so this was said one of the problems for future medical and clinical application.

iMartix can be used for not only stem cells, but various cells, like epidermal cells, vascular endothelial cells.

Fig. Human Epidemal Cells and Vascular Endothelical Cells cultured on iMartix-511.
More efficient adhesion and extension can be seen when cultured on iMatrix-511.

Features

• Simple Handling
• Can be used for various cells - especially iPS / ES cells
• Reduce the risk of contamination by xeno-components in your culture system.
• High cell viability and proliferation rate.
• 175 μg of iMatrix?511 can coat about 2 to 6 of 6 well plates.

How to use

1. Dilute iMatrix-511 solution with PBS (-) and put onto the cell culture surface at a final concentration of 0.1-1.5 µg/cm2. ※The optimal concentration for coating varies with the type of cells, cell lines, and the culture medium used. For the first time usage, start with the final concentration of 0.5 µg/cm2 and determine the optimal condition for coating.
2. Incubate in room temperature for 3 hours and discard the solution.
3. Put cells and culture medium to start cell culture.