iMatrix-511 Silk (0.5 mg/ml solution) (6x175 ug)

Product#: 892021
$350.00
Availability:
Ships in 1-2 weeks

iMatrix-511 Silk (0.5 mg/ml solution) 

Cat. No. 892021
Format Liquid Solution (solvent:PBS(-))
Size 6 x 175 μg
Stroage 4°C,Dark Storage 


Description

iMatrix-511 (Laminin-511 E8) is beneficial for your stem cell culture!

iMatrix-511, is the recombinant human Laminin-511 E8 fragment, produced by CHO-S cells and it can reduce the risk of the xeno-components contamination in the culture. Handling is very easy - just dilute in PBS(-), coat it in the culture dish or plate and incubate at room temparature for 3 hours or at 4°C over night.

892011_fig3.jpg


Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals, and play an important role for cell adhesion and proliferation.

Laminin is composed of three parts ; alpha, beta, and gamma - chain and 15 types of laminin is already known. Among them, Laminin composed by Alpha5-chain, Beta1-chain, and Gamma1-chain, is called "Laminin-511".
Laminin-511 is recognized by alpha6beta1 integrin. Cell adhision, extension and proliferation is caused by many cell signalings through intergrin.

Laminin-511 E8 is the fragment of Laminin-511 obtained by enzyme digestion.
Although this is fragment, it has binding ability to alpha6beta1 integrin.
According to Miyazaki's report ( Nat. Commun. 3, 1236 (2012)), Laminin-511 E8 could be used for cell culture of ES cells and iPS cells.
892011_fig1.jpg

To date, mouse feeder cells or matrigel have been used for the culture of iPS cells and ES cells.
However, these cell culture substrates have a risk to bring xeno-components in the culture condition so this was said one of the problems for future medical and clinical application.

iMartix can be used for not only stem cells, but various cells, like epidermal cells, vascular endothelial cells.
892011_fig2.jpg
Fig. Human Epidemal Cells and Vascular Endothelical Cells cultured on iMartix-511.
More efficient adhesion and extension can be seen when cultured on iMatrix-511.

Features

  • Simple Handling
  • Can be used for various cells - especially iPS / ES cells
  • Reduce the risk of contamination by xeno-components in your culture system.
  • High cell viability and proliferation rate.
  • 175 μg of iMatrix?511 can coat about 2 to 6 of 6 well plates.

How to use

  1. Dilute iMatrix-511 solution with PBS (-) and put onto the cell culture surface at a final concentration of 0.1-1.5 µg/cm2. ※The optimal concentration for coating varies with the type of cells, cell lines, and the culture medium used. For the first time usage, start with the final concentration of 0.5 µg/cm2 and determine the optimal condition for coating.
  2. Incubate in room temperature for 3 hours and discard the solution.
  3. Put cells and culture medium to start cell culture.

Differences between iMatrix-511 and iMatrix-511 silk
 

iMatrix_511

iMatrix-511 silk

Source of the material
(Recombinant protein expression system)

Recombinant CHO-S cell

Transgenic silkworms

Raw material

CHO-S cell culture supernatant

Silkworm cocoon

Product grade

Experimental

Experimental

Transgene

Human laminin 511-E8 fragment

Purity

≥ 95 %

Concentration

0.5 mg/mL

Dissociation constant

≤ 10 nM

Expiration date

2 years after the production date

Use for iPS cell culture

iPS cell production and maintenance are possible when used at 0.5 μg/cm2 for coating

 
892021_fig1.png  

Human recombinant Laminin-511 E8 with the same functions and performance as those of iMatrix-511.
Newly launched at competitive price.

This product is purified from silkworm cocoon in which genes of human Laminin-511 E8 fragment is incorporated. With equal integrin-binding activity as iMatrix-511 produced by CHO-S cells, this product can be used for producing and maintaining cultures of iPS cells. 
Highly productive transgenic silkworms makes recombinant laminin fragment at competitive price. With uncoated pre-mix method, the price is comparable to those of Matrigel and vitronectin.

892021_fig2.png 

892021_fig3.png 

Reference

  • Miyazaki et al. Efficient adhesion culture of human pluripotent stem cells using laminin fragments in an uncoated manner. Scientific Reports7, 41165, (2017).  宮崎隆道, 末盛博文 (2017)「簡便かつ低コスト化を?現したヒト多能性幹細胞の培養法」, 35(19), pp3273-78.


 

iMatrix™ Cell Culture Substrates
Product Name Product ID Description Usage Size Link
imatrix221_F0.png iMatrix-221 892061 Recombinant Human Laminin-211
E8 Fragments
Cardiomyocytes 2 × 175 μg Go_Button
892062 6 × 175 μg Go_Button
iMatrix411_F1.png iMatrix-411 892041 Recombinant Human Laminin-411
E8 Fragments
Endothelial cells 2 × 175 μg Go_Button
892042 6 × 175 μg Go_Button
iMatrix511_F1.png iMatrix-511 892011 Recombinant Human Laminin-511
E8 Fragments
Pluripotent Stem Cells 2 × 175 μg Go_Button
892012 6 × 175 μg Go_Button
iMatrix_511_Silk_F1.png iMatrix-511 Silk 892021 Recombinant Human Laminin-511
E8 Fragments, expressed in Silkworm
Pluripotent Stem Cells 6 × 175 μg Go_Button


 

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