Liquid Cell Culture Medium

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Product Descriptions

Leibovitz's L-15 Medium, 2X, Liquid Cell Culture Medium

Leibovitz’s Medium was originally formulated for use in carbon dioxide (CO2) free systems requiring sodium bicarbonate.  This media is buffered by the use of free basic amino acids, phosphate buffers and higher levels of galactose and sodium pyruvate to help maintain physiological pH control.  Due to this composition, the medium does not require supplementation with sodium bicarbonate and can be used under conditions of free gaseous exchange with the atmosphere.  The formulation will maintain physiological pH control. L-15 has been shown to work for human tumor cells and embryonic cells and for established cell lines such as HeLa and Hep-2. The medium is frequently used in diagnostic virology, where tissue cell lines or strains must be grown in closed systems. Leibovitz’s medium obviates the need for frequent medium change.

Medium 199 w/ Earle's Salts, L-Glutamine and Sodium bicarbonate, 2X, Liquid Cell Culture Medium

Medium 199 was the first nutritionally defined medium developed by Morgan, Morton, and Parker in 1950. This complex medium was formulated specifically for nutritional studies on primary chick embryo fibroblasts in the absence of any additives. It was observed that explanted tissue could survive in Medium 199 without serum but long term cultivation of cells required supplementation of the medium with serum.

Medium 199 is formulated with either Hank's salts or Earle's salts. The medium when supplemented with serum can be used for growth of a wide variety of cells. Medium 199 is presently used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells.
 

Image	Cat.No:	Size(mL)	Storage	Link
	DCP-LL152X	500&1000	Dark Storage,2-8°C
	DCP-M199ESLGSB2X