Serratia marcescens Nuclease, Recombinant
Cat. No FNK-RP-smNuc-S
Size: 2500KU
Storage -20℃
Description
Serratia marcescens Nuclease is a recombinant protein with significant applications in molecular biology and biotechnology. This enzyme, also known as NucA nuclease, is a non-specific endonuclease that hydrolyzes internal phosphodiester bonds between nucleotides in nucleic acids[1][2].
Key features of the recombinant S. marcescens Nuclease include:
1. Function: It digests both single and double-stranded RNA and DNA without apparent sequence preference[3].
2. Product: The enzyme produces 5'-monophosphate oligonucleotides, typically 3-8 bases in length[1][2].
3. Applications: It is widely used in the purification of recombinant proteins by reducing or eliminating nucleic acids from disrupted cells, which decreases the viscosity of the resulting solution and facilitates subsequent purification procedures[3].
4. Structure: The recombinant protein is a single, non-glycosylated polypeptide chain containing 245 amino acids with a molecular mass of approximately 30 kDa[5].
5. Source: While the native enzyme is secreted by Serratia marcescens, the recombinant version is typically produced in Escherichia coli[2][5].
6. Stability: For long-term storage, it is recommended to add a carrier protein like HSA or BSA to enhance stability[5].
7. Specificity: The enzyme is highly non-specific, attacking all types of nucleic acids including single-stranded, double-stranded, circular, and supercoiled DNA and RNA[5].
8. Activity: One unit of the enzyme is defined as the amount that causes a ΔA260 of 1 in 30 minutes, corresponding to the complete digestion of 37 μg of DNA under standard reaction conditions[5].
The recombinant S. marcescens Nuclease is commercially available from various suppliers, often with different formulations or fusion tags to suit specific research needs[1][2][3][4].
Citations:
[1] https://www.rndsystems.com/products/recombinant-s-marcescens-nuca-protein-cf_10038-na
[2] https://www.bio-techne.com/p/proteins-enzymes/recombinant-s-marcescens-nuca-nuclease-protein-cf_10038-na
[3] https://www.kerafast.com/item/1752/serratia-marcescens-dnarna-nonspecific-nuclease-expression-plasmid
[4] https://www.origene.com/catalog/proteins/recombinant-proteins/tp790185/nuclease-serratia-marcescens-recombinant-protein
[5] https://www.prospecbio.com/benzonase-nuclease
