Recombinant HRV 3C Protease (His-tag)

Recombinant HRV 3C Protease (His-tag)

Cat. No.: SB-G3410-1000U
Size: 1000U


Description

Recombinant HRV 3C Protease is a recombinant expression of Human Rhinovirus type 14 3C Protease with His tag in Escherichia coli. It is a commonly used cysteine protease. It can specifically recognize the octapeptide sequence L-E-V-L-F-Q-G-P (LeU-Glu-Val-Leu-Ph-Gln-Gly-Pro) or the core pentapeptide sequence L-F-Q-G-P (Leu-Ph-Gln-Gly-Pro) at low temperature. The enzyme digestion was performed between Q (Gln) and G-P (Gly-Pro) amino acid residues. The enzyme has strong specificity and high activity, and it still has high activity even at low temperature of 4℃.

• Application: It is often used to remove MBP, GST, His or other tag proteins from fusion proteins. The protease itself carries a His tag that binds to the nickel column.
• Source: Human Rhinovirus type 14 3C Protease, recombinant expressed in Escherichia coli, containing His tag.
• Enzyme activity : It was defined as one unit of enzyme activity required to cleave more than 90% of 100 μg of substrate (protein containing HRV 3C Protease restriction site) within 16 h at 4 ° C and pH 7.5.
• Inactivation or inhibition: HRV 3C Protease activity was inhibited by 100 mM ZnCl₂, 4 mM AEBSF, and 100 μM Chymostatin.
• Purity and concentration: Purity ≥95% by SDS-PAGE, 2 U/μL.
• Recombinant HRV 3C Protease storage solution: 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol, pH 8.0.
• 10×HRV 3C Buffer：500 mM Tris-HCl，1.5 M NaCl，10 mM EDTA，10 mM DTT，pH 7.5.

Storage and transportation

Transport in wet ice packs; store at -20°C, valid for 12 months.

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