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Rat Agrin(AGRN) ELISA kit
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Rat Agrin(AGRN) ELISA kit
Sandwich quantitative immunoassay for Rat Agrin(AGRN) ELISA kit in rat serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Uniprot No. | P25304 |
| Species | Rattus norvegicus (Rat) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 78 pg/mL-5000 pg/mL |
| Sensitivity | 19.5 pg/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Neuroscience |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
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Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Rat Agrin(AGRN) ELISA kit in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 78 pg/mL-5000 pg/mL with a minimum detectable dose of 19.5 pg/mL.
The Rat Agrin (AGRN) ELISA kit is a reliable and accurate tool for measuring Agrin protein levels in samples from Rattus norvegicus (Rat). Agrin, encoded by the AGRN gene, is a protein that plays a crucial role in the development and maintenance of the neuromuscular junction.
This ELISA kit provides a simple and sensitive assay to quantify Rat Agrin in serum, plasma, and tissue homogenates with a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5 pg/mL. The assay can be completed in just 1-5 hours, making it an efficient and convenient tool for research purposes.
- The expression of agrin was studied during muscle development in a novel three-dimensional muscle-neuronal coculture system. PMID: 12955494
- Agrin deletion mutants show that axonal targeting of agrin depends on multiple domains that function in an additive fashion, including the very N-terminal portions and the C-terminal half of the molecule. PMID: 14697677
- Agrn becomes concentrated at neuroeffector and neuromuscular juncions in developing urinary bladder. PMID: 15711988
- Agrin may play a pivotal role in synaptogenesis in promoting a rapid switch between electrical coupling and synaptic neurotransmission. PMID: 15883200
- Agrin plays a role as a regulator of intercellular communication particularly junctional electric coupling and synapatic remodeling. PMID: 16274639
- Results suggest that by positively regulating filopodia in developing neurons, transmembrane agrin may influence the pattern of neurite outgrowth and synapse formation. PMID: 16860570
- Agrin, released from neurons, controls the maturation of the excitation-contraction coupling mechanism and due to modulation of RyRs and L-type Ca(2+) channels. A role for neural agrin in skeletal muscle maturation is proposed. PMID: 18003748
- Agrin plays an important role in the sprouting phase of reactive synaptogenesis; both its expression and distribution are correlated with extent of successful recovery after TBI. PMID: 18627255
- increasing the concentration of an ECM molecule, i.e. agrin, may enhance nicotine effects on pCREB PMID: 18639611
- transient post-ischemic hypothermia stabilizes brain vessels and reduces blood brain barrier disruption in part by preventing proteolytic degradation of regulatory basement membrane constituents, SPARC and agrin PMID: 19285050
- The results suggest a physiological role for Tm-agrin in the maturation of hippocampal neurons involving positive regulation of dendritic filopodia and promotion of synaptogenesis, but also suggest a role for axonal agrin in synaptogenesis. PMID: 19524020
- different regions within the agrin protein are responsible for synapse formation at the neuromuscular junction and for process formation in central nervous system neurons PMID: 19940118
- Agrin binds BMP2, BMP4 and TGFbeta1 with relatively high affinity. PMID: 20505824
- a model wherein the z8 insert is a Ca(2+)-responsive allosteric element that is essential in forming an active conformation in neuronal a PMID: 20566625
- agrin modulates neuropathic pain through NR1 phosphorylation in GABA neurons PMID: 21068333
- present the first crystal structure of an agrin-LRP4 complex which is essential for neuronal agrin-induced acetylcholine receptor (AChR) clustering PMID: 22302937
- compared expression of agrin isoforms with the expression of AChE-T and ColQ in the developing spinal cord at the stages before and after the formation of NMJ basal lamina PMID: 23117006
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| To assess the linearity of the assay, samples were spiked with high concentrations of rat AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:2 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:4 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 86-91 | |||||
| The recovery of rat AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 97-103 | ||||
| EDTA plasma (n=4) | 96 | 93-99 | ||||