Tris-Tricine SDS Running Buffer [1X]
Cat No.: DCP-TTSDSRB1X
Size: 500 mL & 1 L
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
READY-to-USE 1X Tris Tricine SDS buffer is a specialized running buffer used in polyacrylamide gel electrophoresis (PAGE) for separating and resolving small proteins and peptides, typically in the range of 1-30 kDa. Tris is a primary amine buffer that maintains the pH during electrophoresis. Tricine is a trailing ion that improves the resolution of small proteins and peptides. Tricine has a lower pKa than glycine, allowing for a lower pH in the running buffer. This lower pH helps minimize protein modifications and improves the separation of small proteins and peptides. SDS is an anionic detergent that denatures and imparts a negative charge to proteins, allowing their separation based on molecular weight. Tris-Tricine SDS running buffer can be used for separating larger proteins in addition to small proteins and peptides, but with some limitations. While the Tris-Tricine SDS buffer system is primarily designed and optimized for resolving small proteins and peptides in the range of 1-30 kDa, it can also be used for larger proteins, typically up to around 100 kDa. Although, the resolution and separation efficiency for the larger proteins may not be as good as Tris-Glycine SDS buffer.
Applications
- Protein Electrophoresis: SDS-PAGE for Low Molecular Weight Proteins: Tris-Tricine SDS running buffer is specifically designed for the separation of low molecular weight proteins and peptides. It is particularly effective for proteins in the range of 1-100 kDa, with optimal resolution for proteins as small as 2 kDa.
- Bronchoalveolar Lavage Fluid Analysis: This buffer is used for the polyacrylamide-based separation of bronchoalveolar lavage fluids, which often contain low molecular weight proteins.
- Western Blotting: High-Resolution Protein Transfer: Tris-Tricine SDS running buffer is used in Western blotting to transfer low molecular weight proteins from gels to membranes, enabling detailed analysis and detection of these proteins.
Advantages:
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Improved resolution of small proteins and peptides compared to the traditional Tris-Glycine SDS buffer system.
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Peptide-SDS complexes migrate more slowly, allowing better separation from SDS micelles that can interfere with peptide resolution.
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Proteins separated using the Tris-Tricine system can be directly sequenced.
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Minimized Interference from SDS Micelles: In Tris-Tricine gels, peptide-SDS complexes move more slowly, allowing SDS micelles to separate completely from peptides. This reduces interference and enhances the resolution of small proteins.
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Versatility: Wide Range of Protein Sizes: Tris-Tricine gels can resolve proteins in a broad range from 1 to 100 kDa, making them suitable for various applications requiring the analysis of small to moderately sized proteins.
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Compatibility with Sequencing: Direct Protein Sequencing: Tricine does not interfere with protein sequencing after transfer to PVDF membranes, making it ideal for applications where subsequent sequencing of proteins is required.
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Stable pH Environment: Consistent pH Levels: The buffer maintains a stable pH environment (pH 8.3), which is crucial for the consistent separation of proteins during electrophoresis.
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Reduced Protein Modifications & Lower pH System: The Tricine buffer system operates at a lower pH compared to Tris-Glycine, which minimizes protein modifications such as deamination and alkylation, preserving protein integrity.
Composition:
| Ingredient |
Concentration |
| Tris |
100 mM |
| Tricine |
100 mM |
| SDS |
0.1% |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 8.3
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
