MES SDS Running Buffer [1X]
Cat No.: DCP-MESSDSRB1X
Size: 500 mL & 1 L
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
READY-to-USE 1X MES SDS Buffer is a running buffer used for SDS-PAGE to separate and resolve small to medium-sized proteins. It is used with Bis-Tris gels for optimal separation of proteins in the range of 5-200 kDa under denaturing conditions. It is valuable for applications like protein biochemistry research, quality control, and molecular weight determination. MES has a lower pKa compared to Tris or MOPS which results in a lower pH. This lower pH environment affects the charge shielding around the SDS-protein complexes, altering their mobility. MES has lower ionic mobility and conductivity compared to other buffers, making it suitable for electrophoresis applications. The unique ionic environment created by MES further enhances the faster migration of smaller proteins. It can be used on larger proteins, but compromise resolution for larger proteins.
Key Applications
- Separation of Low Molecular Weight Proteins and Peptides: designed for separating proteins and peptides in the range of 1-100 kDa, with optimal resolution for proteins as small as 1.5-2 kDa.
- SDS-PAGE: separation of small proteins in their denatured state
- Western Blotting: transferring low molecular weight proteins from gels to membranes.
- Analysis of Bronchoalveolar Lavage Fluids: polyacrylamide-based separation of proteins in bronchoalveolar lavage fluids.
Advantages:
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MES substitutes for Tris-glycine, providing better resolution and faster migration for small to medium proteins compared to Tris-glycine buffers.
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The faster migration in MES is advantageous for separating and resolving smaller proteins that may run off the gel in Tris-Glycine or MOPS buffers.
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The different ionic composition of MES compared to other buffers like Tris or MOPS contributes to changes in the ionic strength and charge shielding effects on the SDS-protein complexes.
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Enhanced Resolution of Small Proteins & Reduced Interference from SDS Micelles:
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Wide Range of Resolving Protein Sizes:broad range from 1 to 100 kDa
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Stable pH Environmen & Compatibility with Downstream Applications: does not interfere with protein sequencing after transfer to PVDF membranes
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Reduced Protein Modifications: works at a lower pH compared to Tris-Glycine, which can help minimize protein modifications such as deamination and alkylation, preserving protein integrity.
Composition
| Ingredients |
Concentration |
| MES |
50 mM |
| Tris base |
50 mM |
| SDS |
0.1% |
| EDTA |
1 mM |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 7.3
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
