Tris-Glycine SDS Buffer [1X]
Cat No.: DCP-TGSB1X
Size: 500 mL & 1 Liter
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Tris-Glycine SDS Buffer is commonly used as a running buffer for SDS-PAGE. Sodium dodecyl sulfate (SDS) denatures and applies a negative charge to the proteins. It is an anionic detergent that unfolds and denatures proteins. It disrupts protein's native structure and reduces them to their primary polypeptide chain. This allows for the separation of proteins based on the molecular weight during electrophoresis. Glycine helps to concentrate protein samples and move them through the stacking layer. Tris and glycine provide ions that allow current flow through the gel and serve as both the anode and cathode buffer. Its alkaline pH helps with effective protein separation.
Advantages:
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It can effectively denature proteins.
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It allows for the separation of proteins based only on the molecular weight.
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It helps with head escape during electrophoresis, which prevents gel deformation.
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It provides ions that allow the flow of current through the gel, enabling electrophoretic separation.
Applications:
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Running buffer for SDS-PAGE.
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Base buffer to block membranes with nonfat dry milk for western blotting.
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Used to denature proteins during electrophoresis.
Composition:
| Ingredients |
Concentration |
| Tris base |
25 mM |
| Glycine |
192 mM |
| SDS |
0.1% |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 8.3 (± 0.05)
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Sterility: Filtered 0.1 micron Twice in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
