Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
READY-to-USE 1X MOPS SDS Running Buffer is used for SDS-PAGE to separate and resolve proteins. MOPS is the zwitterionic buffer component that maintains the pH around 7.7. It contains SDS as a denaturing agent to ensure proteins remain unfolded during electrophoresis. MOPS SDS Running Buffer offers improved resolution for larger proteins, a wider separation range, compatibility with Bis-Tris gels, and the provision of denaturing and reducing conditions for efficient protein separation and analysis. MOPS substitutes for glycine in the running buffer and allows better resolution for separating medium to large size proteins. It is commonly used with Bis-Tris gels for optimal separation of proteins under denaturing conditions. The higher pH of MOPS buffer results in slower migration of SDS-protein complexes compared to MES buffer operating at lower pH. This allows more time for separation and better resolution, especially for larger proteins. The ionic composition of MOPS buffers differs from MES or Tris-Glycine buffers which contributes to changes in ionic strength and charge shielding effects on the SDS-protein complexes.
Applications
SDS-PAGE: primarily used for running proteins on Bis-Tris gels in SDS-PAGE, particularly for separating medium- to large-sized proteins.
1D Gel Electrophoresis: suitable for one-dimensional gel electrophoresis, which is widely used in protein analysis and research.
Western Blotting: transfer proteins from gels to membranes, facilitating the detection and analysis of specific proteins.
Advantages:
The higher pH environment affects the charge shielding around the SDS-protein complexes, allowing slower migration of larger proteins.
MOPS SDS buffer can effectively separate a wider range of protein sizes from medium to large.
MOPS SDS buffer is specifically formulated to be used with Bis-Tris polyacrylamide gels, which have a higher pH range.
Reduced Protein Modifications: Minimizes Protein Modifications: The buffer's composition helps minimize protein modifications such as deamination and alkylation, preserving the integrity of proteins during electrophoresis.
Versatility: Compatible with Various Gels: MOPS SDS running buffer can be used with different types of gels, including NuPAGE Bis-Tris gels, making it versatile for various experimental setups.
Slower Protein Migration: Controlled Protein Migration: Compared to MES buffer, MOPS buffer allows proteins to run slower, which can be advantageous for resolving larger proteins more effectively.
Composition:
Ingredients
Concentration
MOPS
50 mM
Tris base
50 mM
SDS
0.1%
EDTA
1 mM
N,N-dimethylformamide
0.01%
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.7
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: 4 ℃
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
