MOPS SDS Running Buffer [1X]
FluxMPS™ MOPS SDS Running Buffer [1X]
FluxMPS™ MOPS SDS Running Buffer [1X] is a ready-to-use, ultrapure electrophoresis buffer built to the same microchannel-safe purity standard Diagnocine applies across its MPS-grade buffer portfolio. Filtered through a quadruple-stage 0.1 µm and 0.04 µm membrane sequence, it delivers a defined 50 mM MOPS / 50 mM Tris base system held near pH 7.7 for consistent SDS-PAGE resolution of medium-to-large proteins on Bis-Tris gels.
- Sterile, ultrapure, filtered 0.1 µm membrane twice and 0.04 µm membrane twice
- Ready-to-use 1X formulation — no dilution or pH adjustment required
- Defined 50 mM MOPS / 50 mM Tris base system holding pH near 7.7
- Compatible with Bis-Tris and NuPAGE-style gels for SDS-PAGE and western blotting
- DNase activity: none detected; RNase activity: none detected
- Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm)
- Customizable concentration, pH, and additive package on request
- pH7.7
- MOPS Concentration50 mM
- Tris Base Concentration50 mM
- SDS Concentration0.1%
- Filtration0.1 µm x2 + 0.04 µm x2
- SterilitySterile, filtered in sterile environment
- DNase ActivityNone detected
- RNase ActivityNone detected
- Storage4°C
- Shelf Life1 year
Engineered where standard running buffers fail
Conventional single-pass 0.22 µm-filtered running buffers can carry subvisible particulates, inconsistent pH, and undocumented nuclease activity into sensitive downstream workflows. FluxMPS™ MOPS SDS Running Buffer [1X] is built to remove those failure points at the source.
Microchannel-safe purity
Quadruple-stage 0.1 µm and 0.04 µm membrane filtration removes fine particulates before the buffer ever reaches your gel tank or downstream instrumentation.
Precise, stable pH
A defined 50 mM MOPS / 50 mM Tris base system holds pH near 7.7, giving reproducible SDS-protein complex migration run to run.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), consistent with USP <85> water-quality expectations for sensitive biological reagents.
Low background for downstream detection
Clear, colorless composition with no detectable DNase or RNase activity supports clean western blot and downstream detection signal.
Defined, traceable composition
MOPS, Tris base, SDS, EDTA, and N,N-dimethylformamide are each present at a stated, lot-consistent concentration.
Customization on demand
Alternate concentrations, pH, or added chemicals, compounds, proteins, and supplements are available on inquiry.
Quadruple-stage filtration system
MOPS SDS Running Buffer [1X] is filtered through two sequential 0.1 µm membrane passes followed by two sequential 0.04 µm membrane passes, delivering a sterile, ultrapure buffer for cell and molecular biology workflows.
- 1
0.1 µm Pre-filtration I
First 0.1 µm membrane pass removes large particulates and aggregates, extending downstream filter life.
- 2
0.04 µm Pre-filtration II
First 0.04 µm membrane pass retains fine particulates and bioburden ahead of final sterile filtration.
- 3
0.1 µm Sterile-filtration I
Second 0.1 µm membrane pass provides redundant particulate reduction in a sterile environment.
- 4
0.04 µm Sterile-filtration II — Final Polish
Second 0.04 µm membrane pass is the final polish, applied in a sterile filling environment.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration passes remove finer particulates than a single conventional 0.22 µm pass, supporting a sterile, DNase- and RNase-free buffer for cell and molecular biology experiments.
© Diagnocine® — DCP-MOPSSDSRB1X
Where MOPS SDS Running Buffer [1X] fits your workflow
READY-to-USE 1X MOPS SDS Running Buffer is used for SDS-PAGE to separate and resolve proteins. MOPS is the zwitterionic buffer component that maintains pH near 7.7, and SDS acts as a denaturing agent so proteins remain unfolded during electrophoresis. MOPS substitutes for glycine in the running buffer and allows better resolution when separating medium- to large-size proteins, and it is commonly paired with Bis-Tris gels for optimal separation under denaturing conditions. The higher pH of MOPS buffer slows migration of SDS-protein complexes compared with MES buffer at lower pH, allowing more time for separation and better resolution of larger proteins. The ionic composition of MOPS buffers differs from MES or Tris-Glycine buffers, contributing to changes in ionic strength and charge shielding around SDS-protein complexes.
Automated Bioreactors & Robotics
For automated liquid-handling and robotics workflows feeding sensitive downstream steps, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available on request for teams that need an even finer particulate exclusion for automated systems.
- Total Particulate Exclusion for automated liquid handling lines
- Valve & Sensor Protection against fine particulate fouling
- Extended Perfusion Stability for long-duration automated runs
Inquiry Required: the 0.01 µm (10 nm) ultra-filtered grade is produced to order — contact support@diagnocine.com to discuss availability for your system.
Micro Physiological System (MPS) & Chip
Ultrapure running buffer for downstream protein analysis of samples recovered from microfluidic and organ-on-a-chip culture systems.
SDS-PAGE Sample Preparation
1X ready-to-use format for lysate loading and gel electrophoresis without additional dilution steps.
iPSC-Derived Model Handling
Supports SDS-PAGE analysis of protein lysates recovered from iPSC-derived cell models.
Endothelial & Primary Cell Perfusion
Used downstream of perfusion-cultured endothelial and primary cell models for protein-level readouts.
SDS-PAGE, Western Blotting & Blocking
Primarily used for running proteins on Bis-Tris gels in SDS-PAGE and for transferring separated proteins to membranes for detection and analysis.
1D Gel Electrophoresis & Protein Analysis
Suitable for one-dimensional gel electrophoresis widely used across protein analysis and research applications.
Specifications at a glance
Measured and declared parameters for MOPS SDS Running Buffer [1X], DCP-MOPSSDSRB1X.
| Parameter | Specification |
|---|---|
| Formulation / Composition | MOPS, Tris base, SDS, EDTA, N,N-dimethylformamide |
| Appearance | Clear, colorless liquid |
| pH USP <791> | 7.7 |
| Molarity / Concentration | MOPS 50 mM; Tris base 50 mM |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment |
| DNase Activity | None detected after incubation of plasmid DNA with this product for 18 hr at room temperature |
| RNase Activity | None detected after incubation of ribosomal RNA with this product for 18 hr at room temperature |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved manufacturing facility |
| Fill Environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Ultrapure reagents |
| Traceability | Final packaging, QA, and testing at the DiagnoCine R&D and Quality Testing Center; customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Manufacturing QMS | ISO 13485-certified |
| Regulatory Alignment | CE-approved facility |
| Production Method | Filtered 0.1 µm membrane twice and 0.04 µm membrane twice |
| Intended Use | Research Use Only (RUO) |
Full composition
Every buffer component and its stated concentration for MOPS SDS Running Buffer [1X], reproduced from the certificate of composition.
| Component | CAS Number | Concentration |
|---|---|---|
| MOPS | 1132-61-2 | 50 mM |
| Tris base | 77-86-1 | 50 mM |
| SDS | 151-21-3 | 0.1% |
| EDTA | 60-00-4 | 1 mM |
| N,N-dimethylformamide | 68-12-2 | 0.01% |
Manufacturing & compliance
MOPS SDS Running Buffer [1X] is produced and released under a documented quality system spanning raw materials through final fill.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) as the base for a low-background running buffer.
ISO Class 5 Fill & Finish
Sterile filtration and fill are performed in a controlled, sterile environment to protect final buffer purity.
Micro-Batch Precision
All final packaging, quality assurance, testing, and customization are completed at the DiagnoCine R&D and Quality Testing Center and DiagnoCine Precision, Totowa, New Jersey, USA.
DNase Activity Lot QC
None detected after incubation of plasmid DNA with this product for 18 hr at room temperature.
RNase Activity Lot QC
No RNase activity detected after incubation of ribosomal RNA with this product for 18 hr at room temperature.
Sterility USP <71>
Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment.
Documentation / CoA
Lot-specific certificates of analysis are available on request.
How DCP-MOPSSDSRB1X compares
A side-by-side look at FluxMPS™ MOPS SDS Running Buffer [1X] against conventional and standard running buffer alternatives.
| Parameter | DCP-MOPSSDSRB1X (FluxMPS™) | Conventional Buffer (0.22 µm filtered) | Standard Alternative (0.22 µm filtered) |
|---|---|---|---|
| Defined MOPS / Tris base molarity | check_circle 50 mM / 50 mM | cancel | cancel |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| DNase / RNase activity tested | check_circle | cancel | cancel |
| Ultrapure Type 1 water base | check_circle | cancel | cancel |
| Manufacturing QMS | ISO 13485-certified | cancel | cancel |
| Bis-Tris / NuPAGE gel compatibility | check_circle | check_circle | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about MOPS SDS Running Buffer [1X], DCP-MOPSSDSRB1X.
Supporting literature
Peer-reviewed literature relevant to MOPS buffer chemistry, SDS-PAGE, western blotting, and microfluidic/organ-on-a-chip protein workflows.
- Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
- Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970. doi:10.1038/227680a0
- Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. PNAS. 1979. doi:10.1073/pnas.76.9.4350
- Schagger H. Tricine-SDS-PAGE. Nat Protoc. 2006. doi:10.1038/nprot.2006.4
- Klose J. Protein mapping by combined isoelectric focusing and electrophoresis. Humangenetik. 1975. doi:10.1007/BF00273841
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Kurien BT, Scofield RH. Western blotting: an introduction. Methods Mol Biol. 2015. doi:10.1007/978-1-4939-2694-7_17
