Tris-Glycine Non-Denaturing Buffer [1X]
FluxMPS™ Tris-Glycine Non-Denaturing Buffer [1X]
FluxMPS™ Tris-Glycine Non-Denaturing Buffer [1X] (DCP-TGNDRB1X) is an MPS-grade, SDS-free native running buffer formulated with 25 mM Tris base and 192 mM glycine at pH 8.3. Manufactured with sequential 0.1 µm and 0.04 µm membrane filtration using ultrapure Type 1 water, it delivers ultra-clean, particulate-controlled buffering for native PAGE, western blotting, and microchannel-based workflows.
- Filtered 0.1 µm membrane twice and 0.04 µm membrane twice for quadruple-stage purity
- Native, non-denaturing Tris-Glycine system — no SDS detergent
- Defined pH 8.3 with 25 mM Tris base and 192 mM glycine
- Sterile, DNase- and RNase-tested for sensitive downstream assays
- Ultrapure Type 1 water (18.2 MΩ·cm) base
- Available in 500 mL and 1000 mL formats
- Custom pH, molarity, and additive formulations available on request
- pH8.3
- Buffer SystemTris-Glycine
- Tris Base25 mM
- Glycine192 mM
- SDS ContentNone (non-denaturing)
- Filtration0.1 µm x2 + 0.04 µm x2
- SterilitySterile-filtered
- Storage4 °C
- Shelf Life2 years
- FormatReady-to-use 1X
Engineered where standard buffers fail
Conventional 0.22 µm-filtered running buffers can carry subvisible particulates that accumulate in gel wells, transfer stacks, and microfluidic channels, and can drift in pH or ionic strength between lots[1,2,3]. FluxMPS™ Tris-Glycine Non-Denaturing Buffer [1X] is built to remove that variability.
Microchannel-safe purity
Sequential 0.1 µm and 0.04 µm membrane filtration is used to minimize particulate carryover into sensitive electrophoresis, blotting, and microfluidic systems.
Precise, stable pH
A defined 25 mM Tris base / 192 mM glycine system is formulated to hold a pH of 8.3, supporting reproducible native protein mobility[8].
Ultrapure-grade water
Formulated in ultrapure Type 1 water (18.2 MΩ·cm) to reduce background contaminants in downstream assays.
Low background for imaging & assays
Non-denaturing, SDS-free composition supports native conformation electrophoresis and clean electroblotting for downstream immunodetection[4].
Defined, traceable composition
Tris base and glycine are lot-controlled, with concentrations verified at 25 mM and 192 mM respectively.
Customization on demand
Alternate concentrations, pH, and added chemicals, compounds, proteins, or supplements are available — contact support@diagnocine.com.
Quadruple-stage filtration system
DCP-TGNDRB1X is manufactured with a quadruple-stage membrane filtration process — filtered 0.1 µm membrane twice and 0.04 µm membrane twice — to deliver ultra-clean, microchannel-safe Tris-Glycine buffer for electrophoresis, blotting, and microfluidic workflows.
-
1
0.1 µm Pre-filtration I
First pass through a 0.1 µm membrane removes large particulates and aggregates, extending the service life of downstream filters.
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2
0.04 µm Pre-filtration II
A 0.04 µm membrane pass retains fine particulates and bioburden ahead of final sterile processing.
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3
0.1 µm Sterile-filtration I
A second 0.1 µm pass provides redundant particulate control prior to the final polish.
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4
0.04 µm Sterile-filtration II — Final Polish
A final 0.04 µm pass delivers the ultimate polish, with fill performed under controlled aseptic conditions.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm membrane filtration removes finer particulates than a single conventional 0.22 µm sterile-filtration pass, supporting cleaner electrophoresis, blotting, and downstream microfluidic use.
© Diagnocine® — DCP-TGNDRB1X
Where DCP-TGNDRB1X performs
As a native, non-denaturing Tris-Glycine running buffer without SDS detergent, this buffer supports native PAGE, western blot, and electroblotting workflows[1,2,3,4], and can also serve as a base for denaturing gel running buffers or Tris-Glycine-Methanol transfer buffer by adding methanol.
Automated Bioreactors & Robotics
For automated microfluidic and robotic liquid-handling platforms[9,10], an optional 0.01 µm (10 nm) ultra-filtered variant of this Tris-Glycine buffer can be requested, engineered to protect microvalves and sensors from residual submicron particulates during long, unattended runs.
- Total Particulate Exclusion
- Valve & Sensor Protection
- Extended Perfusion Stability
Inquiry Required: contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade for automated bioreactor and robotic platforms.
Micro Physiological System (MPS) & Chip
Ultra-filtered Tris-Glycine buffer suited to particulate-sensitive microfluidic channels and chip-based platforms.
Wash, Dilution & Reconstitution
A defined-pH Tris-Glycine base for wash and dilution steps ahead of electrophoresis or blotting protocols.
iPSC-Derived Model Handling
Low-particulate buffer background suited to downstream protein analysis of iPSC-derived cell models.
Endothelial & Primary Cell Perfusion
Compatible with protein analysis workflows following perfusion-based vascular and primary cell culture experiments.
ELISA, Blotting & Blocking
Native Tris-Glycine chemistry is a core component of western blot and electroblotting workflows[5,6].
Microscopy & Optical Sensing
Ultra-clean formulation reduces particulate background relevant to optical and biosensor-based readouts.
Specifications at a glance
Values below reflect DCP-TGNDRB1X as manufactured and quality-controlled by Diagnocine.
| Parameter | Specification |
|---|---|
| Formulation | Tris base 25 mM; Glycine 192 mM (non-denaturing, SDS-free) |
| Appearance | Clear, colorless liquid |
| pH (USP <791>) | 8.3 |
| Buffer System | Tris-Glycine |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Sterile; filtered 0.1 µm membrane twice and 0.04 µm membrane twice |
| DNase Activity | None detected (18 hours, room temperature, plasmid DNA challenge) |
| RNase Activity | None detected (18 hours, room temperature, ribosomal RNA challenge) |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Fill Environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4 °C |
| Shelf Life | 2 years |
| Detergent Content | None (SDS-free, non-denaturing formulation) |
| Methanol Content | Not included; add 20% methanol to prepare Tris-Glycine-Methanol transfer buffer |
| Parameter | Specification |
|---|---|
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facilities |
| Regulatory Alignment | ISO 13485:2016 |
| Production Location | Final packaging, QA, and customization performed in Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
Component concentrations for DCP-TGNDRB1X, expressed as manufactured.
| Component | CAS Number | Concentration |
|---|---|---|
| Tris base | 77-86-1 | 25 mM |
| Glycine | 56-40-6 | 192 mM |
Manufacturing & compliance
DCP-TGNDRB1X is produced and quality-tested under a controlled, certified manufacturing chain.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated with 18.2 MΩ·cm Type 1 water as the base for all buffer components.
ISO Class 5 Fill & Finish
Filtered and filled under controlled aseptic conditions following quadruple-stage membrane filtration.
Micro-Batch Precision
All final packaging, quality assurance, testing, and customization are completed at DiagnoCine Precision in Totowa, New Jersey, USA.
DNase & RNase Testing
No DNase or RNase activity detected after 18-hour incubation of plasmid DNA and ribosomal RNA at room temperature, respectively.
Sterility Assurance USP <71>
Sterile-filtered via 0.1 µm membrane twice and 0.04 µm membrane twice.
Certificate of Analysis
A CoA documenting lot-specific QC results is available on request.
Regulatory Alignment
Produced under ISO 13485:2016-aligned, CE-approved manufacturing.
How DCP-TGNDRB1X compares
A qualitative look at how DCP-TGNDRB1X compares to conventional Tris-Glycine running buffers.
| Parameter | DCP-TGNDRB1X (FluxMPS™) | Conventional Buffer | Standard Alternative |
|---|---|---|---|
| Formulation | Native, non-denaturing Tris-Glycine (SDS-free) | Often includes SDS | Often includes SDS |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Typically deionized | Typically deionized |
| Manufacturing QMS | check_circle ISO 13485:2016 | cancel Not always certified | cancel Not always certified |
| DNase / RNase tested | check_circle Yes | cancel Not always disclosed | cancel Not always disclosed |
| Custom formulation | check_circle Available on request | cancel Limited | cancel Limited |
Frequently asked questions
Common questions about DCP-TGNDRB1X.
Supporting literature
Curated literature relevant to Tris-Glycine buffer chemistry, native and denaturing electrophoresis, protein transfer, and microfluidic applications.
- Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970. doi:10.1038/227680a0
- Ornstein L. Disc electrophoresis-I: Background and theory. Ann N Y Acad Sci. 1964. doi:10.1111/j.1749-6632.1964.tb14197.x
- Davis BJ. Disc electrophoresis-II: Method and application to human serum proteins. Ann N Y Acad Sci. 1964. doi:10.1111/j.1749-6632.1964.tb14182.x
- Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc Natl Acad Sci U S A. 1979. doi:10.1073/pnas.76.9.4350
- Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012. doi:10.4103/1947-2714.100998
- Kurien BT, Scofield RH. Western blotting. Methods. 2006. doi:10.1016/j.ymeth.2005.11.008
- Bass JJ, et al. An overview of technical considerations for Western blotting applications to physiological research. Scand J Med Sci Sports. 2017. doi:10.1111/sms.12702
- Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014. doi:10.1038/nature13118
