Tris-Glycine Non-Denaturing Buffer [1X]
Cat No.: DCP-TGNDRB1X
Size: 500 mL & 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
- World’s cleanest buffer for all cell and molecular biology experiments
Description:
Tris-glycine buffer is a native, non-denaturing running buffer WITHOUT SDS detergent used in western blot. It can be used as a base buffer to make denaturing gel running buffers or transfer buffers by adding methanol. Sample prep proteins will run in native conformation with a native PAGE set-up. Glycine helps to concentrate protein samples and move them through the stacking layer. Tris and glycine provide ions that allow current flow through the gel and serve as both the anode and cathode buffer. Its alkaline pH helps with effective protein separation.
Additionally, it is used to make Tris-glycine-methanol transfer buffer which is used as protein transfer buffer for wet blot transfer. Methanol helps to prevent gel swelling during transfer and improves the efficiency of protein adsorption onto the membrane.
It might not be suitable for transfer of very high molecular weight proteins which require lengthy transfer time.
*This product does not contain Methanol. Please add 20% methanol to make the Tris Glycine Methanol Transfer Buffer.
Advantages:
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The specific ionic composition and pH of the buffer enables the mobility of negatively charged proteins towards the positive electrode during the electroblotting process.
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It is compatible with most commonly used membranes.
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It helps with protein transfer from gel to membrane.
Composition:
| Ingredients |
Concentration |
| Tris base |
25 mM |
| Glycine |
192 mM |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 8.3
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Sterility: Filtered 0.1 micron Twice + 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
