Cat. No. DM660 L Size 5x1.2 ml Storage 4 °C Stability 12 months at 4 °C Storage Buffer Tris-HCl (pH6.8), EDTA, Glycerol, SDS
Protein MultiColor Stable Ⅱ *This is a renewal product of Protein MultiColor Ladder Marker, Stable.
Renewal Points !
*The product name changes from "Stable" to "StableⅡ".
*Aprotinin (Green) of 8.5kDa is newly added.
*The loading amount per one lane changes from 5ul to 10ul. Number of loads do not change, 120 times.
Description
Pre-stained protein ladder for broad range of proteins (8 to 230 kDa), stable in refrigerator ( at 4℃ ) for 1 year !
The DynaMarker® Protein MultiColor Stable II is a pre-stained protein molecular weight marker. The marker has a remarkable feature that it is possible to store at 4 °C. The feature allow us to start electrophoresis with the marker, because it is always in a liquid state while stored at 4 °C. The DynaMarker® Protein MultiColor Stable II consists of nine prestained proteins. Each of them are stained red, blue, purple, green or orange, ranging in apparent molecular weight from approximately 8 kDa to 230 kDa. The DynaMarker® Protein MultiColor Stable II is suitable for visualizing proteins during electrophoresis without staining and for monitoring electrophoretic transfer onto membranes. The protein concentrations are optimized to give uniform band intensities. The marker is supplied in gel loading buffer for direct loading onto SDS-PAGE without heating or adding reducing agents.
Electrophoresis profile of DynaMarker Protein MultiColor Stable II (10 μl) on 6% polyacrylamide (5% C) Gel / AllView PAGE Buffer (Code#DS520) as running buffer.
Feature
Multicolored pre-stained natural proteins
Stable for up to 1 year at 4℃
Ready-to-load (10μL / lane)
Sharp bands
M.W. range from approx. 8 to 230 kDa
Applications
Real-time monitoring of protein mobility on SDS-PAGE gel.
Can check transfer efficiency on membranes without staining.
Protocol
Take the marker out of refrigerator.
Load 10 μl for mini-gels or more for large size gels.
Load your samples.
Start electrophoresis.
Note There is no need to heat or add reducing agents
Contents
Apparent molecular weight (kDa) *
Protein
Tris-Glycine-SDS buffer (Laemmli running buffer)
AllView PAGE Buffer (Code#DS520)
Myosin
249
250
β-Galactosidase
137
141
Phosphorylase-b
96
100
BSA
73
71
Ovalbumin
46
45
Carbonic anhydrase
31
30
Soybean trypsin inhibitor
26
25
Lysozyme
18
17
Aprotinin
6.3
8.4
Note
As covalently bound dye affects protein mobility, each batch of prestained protein marker is calibrated against
unstained standards. A prestained protein marker should be used for approximate molecular weight determination. For
precise molecular weight determination use an unstained molecular weight marker.
* : The apparent molecular weight values are lot specific and depends on the electrophoresis running buffer.
Comparison
Supplier
BioDynamics Laboratory (BDL)
Company A
Company B
Company C
Company D
Number of loads
120
50
50
50
50
Storage Temp.
4℃
-20℃
-20℃
4℃
4℃
Shelf Life
1 year
3 months
1 year
4 months
3 months
Related product
Code
Product Name
Description
DS520
All View PAGEBuffer
AllView PAGE Buffer is a new type of running buffer for SDS-PAGE electrophoresis. This buffer is possible to separate proteins with wide range of molecular weights in the basic Laemmli gel (Tris-HCl) similar to using “gradient gel”.