Plant DNA Midi Kit (10 Preps)

Product#: BMG-GD2612-01
$95.00
Availability:
Ships in 1-2 Weeks

Plant DNA Midi Kit (10 Preps)

Cat. No.: BMG-GD2612-01
Size: 10 Preps

Description: 

The Plant DNA Midi Kit is designed for medium-scale isolation of DNA from plant samples. It is particularly suitable for researchers who require DNA extraction from larger quantities of plant material compared to mini kits. 

Applications:
  • The high-quality DNA extracted using the kit is suitable for PCR, which is used to amplify specific DNA sequences for further analysis.
  • The kit's DNA can be used in qPCR, allowing researchers to quantify DNA amounts in real-time, which is crucial for gene expression studies.
  • Researchers can use the DNA for genotyping to study genetic variations within and between plant species.
  • The DNA is suitable for Southern blotting, a method used to detect specific DNA sequences within a DNA sample.
  • The extracted DNA can be prepared for NGS, enabling comprehensive genomic studies and the identification of genetic markers.
  • The DNA can be used for RFLP analysis, which helps in identifying variations in homologous DNA sequences.
Features:
  • The kit can process up to 500 mg of wet plant tissue or 125 mg of dry tissue, allowing for the extraction of DNA from larger sample sizes compared to mini kits.
  • It is designed to efficiently recover genomic DNA up to 60 kb in size from both fresh and dried plant tissue samples. 
  • The kit utilizes spin column technology, which combines the reversible nucleic acid-binding properties of the matrix with speed and versatility. This technology helps eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.
  • The purified DNA is of high quality and suitable for various downstream applications, including PCR, restriction digestion, and hybridization techniques. 
  • The kit provides a straightforward method for DNA isolation, making it accessible for researchers without the need for complex equipment or procedures.
Reference: 

Huang, W., Lin, Z., Zhang, Z., & Chen, J. (2021). First Report of Colletotrichum fructicola Causing Anthracnose on Water Hyacinth in China. Plant Disease105(8), 2246. https://doi.org/10.1094/pdis-12-20-2621-pdn

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