Plant RNA Midi Kit (25 Preps)

Plant RNA Midi Kit (25 Preps)

Cat. No.: BMG-R6612-02
Size: 25 Preps
 

Description:

The Plant RNA Midi Kit is a specialized kit designed for the extraction and purification of RNA from plant tissues.

• The kit is suitable for preparing RNA for reverse transcription PCR, which is used to study gene expression by converting RNA into complementary DNA (cDNA) and amplifying specific DNA targets.
• This technique involves the detection of specific RNA molecules among a mixture of RNA. The kit provides RNA suitable for this application, allowing researchers to study RNA size and abundance.
• The kit can be used to isolate mRNA, which is essential for studying gene expression and conducting experiments that require mRNA as a starting material.
• These assays are used to map the boundaries of RNA transcripts and study RNA processing events. The high-quality RNA obtained from the kit is suitable for such assays.
• The kit provides RNA that can be used for in vitro translation, a process that synthesizes proteins from RNA templates outside of living cells.

• The kit is designed to isolate high-quality RNA suitable for various downstream applications such as RT-PCR, Northern blotting, and in vitro translation. It ensures the removal of contaminants like polysaccharides and phenolic compounds that can interfere with RNA quality. 
• The use of silica-membrane spin columns facilitates the binding and purification of RNA, allowing for efficient processing and high yields of RNA from plant tissues.
• The kit eliminates the need for phenol/chloroform extractions and other time-consuming steps like CsCl gradient ultracentrifugation, simplifying the RNA extraction process.
• The kit offers a quick workflow, enabling RNA isolation with genomic DNA removal in less than 40 minutes, which is efficient for laboratories requiring fast processing times.
• The kit typically has a high binding capacity, allowing for the purification of large amounts of RNA, up to 1 mg, from plant tissues.