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Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit
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Mouse Plasma protease C1 inhibitor(SERPING1) ELISA kit
Sandwich quantitative immunoassay for serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 in mouse serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 |
| Uniprot No. | P97290 |
| Species | Mus musculus (Mouse) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 5.6 ng/mL-360 ng/mL |
| Sensitivity | 1.4 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Blood Coagulation |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 5.6 ng/mL-360 ng/mL with a minimum detectable dose of 1.4 ng/mL.
This Mouse SERPING1 ELISA Kit was designed for the quantitative measurement of Mouse SERPING1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 5.6 ng/mL-360 ng/mL and the sensitivity is 1.4 ng/mL.
- Transgenic mice have been generated that express human C1 inhibitor mRNA and protein under the control of the human promoter and regulatory elements; C1nh production is demonstrated in mouse brain, spleen, liver, heart, kidney, and lung. PMID: 12421980
- In vivo, C1 inhibitor (C1INH) reduces the number of viable bacteria in the blood and peritoneal fluid and accelerates killing of bacteria by blood neutrophils and peritoneal macrophages. PMID: 17785834
- C1-inhibitor limits neointimal plaque formation and inflammation. This may involve blockade of complement activation, inhibition of leukocyte recruitment, and reduced triglyceride levels PMID: 18071075
- In addition to the protective activities mediated via inhibition of the complement system, these studies indicate that C1INH also plays a direct role in suppression of leukocyte transmigration into reperfused tissue. PMID: 18787060
- C1INH prevented myocardial ischemia reperfusion injury in mice. PMID: 22705194
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SERPING1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:2000 | Average % | 104 | ||||
| Range % | 101-107 | |||||
| 1:4000 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:8000 | Average % | 98 | ||||
| Range % | 95-103 | |||||
| The recovery of mouse SERPING1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 85-92 | ||||
| EDTA plasma (n=4) | 96 | 93-99 | ||||