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Mouse Free Thyroxine,FT4 ELISA Kit
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4 core reagents below are included with every order, plus 1 of the 2 detection-specific reagents (matched automatically to your kit type) — 5 components, every time.
+ the 5th component, matched to your kit's detection method:
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Mouse Free Thyroxine,FT4 ELISA Kit
Competitive quantitative immunoassay for Free Thyroxine,FT4 in mouse serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | Free Thyroxine,FT4 |
| Species | Mus musculus (Mouse) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 4 pmol/L-64 pmol/L |
| Sensitivity | 1.08 pmol/L |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Competitive (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Others |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Competitive binding
450 nm detection
Standard curve
Quantification
In this competitive ELISA, free Free Thyroxine,FT4 in the sample competes with plate-bound antigen for antibody binding. Signal intensity is inversely proportional to analyte concentration. Final quantification is derived from a standard curve fitted using Curve Expert software, covering 4 pmol/L-64 pmol/L with a minimum detectable dose of 1.08 pmol/L.
Free thyroxine (FT4) represents the unbound, metabolically active fraction of thyroxine hormone in circulation, constituting approximately 0.03% of total thyroid hormone. As the primary hormone produced by the thyroid gland, FT4 regulates cellular metabolism, growth, and development throughout the body. Unlike total thyroxine measurements, FT4 levels provide a more accurate picture of thyroid function since they remain unaffected by variations in thyroid-binding proteins. This makes FT4 an important biomarker in endocrine research and thyroid disorder studies.
The Mouse Free Thyroxine FT4 ELISA Kit (CSB-E05080m) measures FT4 concentrations in mouse serum, plasma, and tissue homogenates. This competitive assay operates within a detection range of 4-64 pmol/L with a sensitivity of 1.08 pmol/L. The kit requires 50-100 μL sample volume and provides results within 1-5 hours using spectrophotometric detection at 450 nm wavelength.
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
