1. correction of bone mineralization abnormalities in knockout mice null for both the TNAP (Akp2) and PC-1 (Enpp1) genes PMID: 12082181
2. The Alpl (Akp2) gene is located within the quantitative trait locus region for alkaline phosphatase activity on chromosome 4. PMID: 16159911
3. alkaline phosphatase 2(Hpp/Hpp) mice develop late-onset skeletal disease, notably defective endochondral ossification and bone mineralization that leads to arthropathies of knees and shoulders PMID: 17539739
4. The process selectively internalizes IAP and may contribute to the appearance of the enzyme in serum and surfactant-like particles. PMID: 17947448
5. Results show that inorganic phosphate (P(i)) levels and TNSALP activity increased in response to androgen/androgen receptor (AR), and P(i) signals increase the expression and translocation of AR. PMID: 18838539
6. TNAP-AID mice expressed alpha-fetoprotein and had deleterious mutations in the tumour suppressor gene Trp53, some of which corresponded to those found in human cancer. PMID: 18997814
7. Tissue-nonspecific alkaline phosphatase is the enzyme that hydrolyzes both ATP and inorganic pyrophosate in matrix vesicles. PMID: 19874193
8. Once the HT2B receptor is expressed, it constitutively controls tissue-nonspecific alkaline phosphatase activity at a post-translational level along the overall period of bone mineral deposition PMID: 20573958
9. These results demonstrate that calcium ions released from apatite are important in the synergistic effect of 20alpha-HC and apatite. PMID: 20683129
10. We show that TNAP knockdown reduces cell proliferation and differentiation into neurons or oligodendrocytes PMID: 20849921
11. Data show that alkaline phosphatase (AP) activity in brain vessels and parenchyma in which AP exhibits specific patterns is attributable to TNAP. PMID: 21191615
12. Results demonstrate that TNAP, regulating both ligand availability and protein expression of P2X7 receptor, is essential for axonal development. PMID: 21289095
13. a link between ATRA-induced mL/B/K-ALP gene transcription and chromatin remodeling PMID: 22270475
14. In the cerebral cortex, myelinated axons, while present in wild-type, were absent in the Akp2( -/- ) mice and these animals also displayed a significantly increased proportion of immature cortical synapses. PMID: 22696173
15. data suggest that the promineralization role of TNAP may be related not only to its accepted pyrophosphatase activity but also to its ability to modify the phosphorylation status of OPN. PMID: 23427088
16. mineralization abnormalities of dentin; reduced overall mineralization with decreased matrix vesicle mineralization in the Phospho1(-/-) mice; almost complete absence of matrix vesicles in mice; further reduction in mineralization. PMID: 23694930
17. Inhibition of rhBMP-2-induced ALP activity by intracellular delivery of SMURF1 in murine calvarial preosteoblast cells. PMID: 24288199
18. Taken together, these data indicate that ATF3 is a novel negative regulator of osteoblast differentiation by specifically suppressing ALP gene expression in preosteoblasts. PMID: 24315873
19. CD73 and TNAP play interactive roles to metabolize luminally applied 5'-AMP in the renal vasculature such that inhibition of both is required to inhibit the production of adenosine. PMID: 24990899
20. Findings demonstrate that Alpl(-/-) mice exhibit a craniofacial skeletal phenotype similar to that seen in infants with HPP, including true bony craniosynostosis in the context of severely diminished bone mineralization PMID: 25014884
21. TNAP plays a role in governing the phosphorylation status of phospholamban in the sarcoplasmic reticulum. PMID: 25015959
22. p107 is required for the efficient recruitment of an activating SWI/SNF chromatin-remodeling complex, an essential event in Alpl induction. PMID: 25182511
23. TNAP in the vasculature contributes to the pathology of medial vascular calcification and that it is a druggable target. PMID: 25428889
24. In cardiac fibroblasts, TNAP expression and activity is induced by sFRP2. PMID: 25972450
25. Prevention of lethal murine hypophosphatasia by neonatal ex vivo gene therapy using lentivirally transduced bone marrow cells expressing Akp-2. PMID: 26467745
26. Despite similar deficiencies in alkaline phosphatase, Alpl(-/-) mice develop craniosynostosis and a brachycephalic/acrocephalic craniofacial shape of variable penetrance. PMID: 26605996
27. These analyses revealed that TNAP deficient mice present an increased proliferation of neural precursors, an altered neuronal morphology, and an augmented neuronal activity. We found that these alterations were associated with a partial downregulation of the purinergic P2X7 receptor (P2X7R). PMID: 27466191
28. TNAP activation in vascular smooth muscle cells (VSMCs) appears sufficient to induce calcification. TNAP activation in VSMCs stimulates expression of chondrocyte markers. PMID: 27932058
29. The results are the first to demonstrate a role for ENC1 in the control of osteoblast differentiation. Additionally, the contrasting mineralization phenotypes and transcriptional patterns seen with coordinate knockdown of both ENC1 isoforms vs selective knockdown of 67 kDa ENC1 suggest opposing roles for the isoforms in regulation of osteoblastic differentiation, through effects on Alpl expression and phosphate cellular PMID: 27996212
30. Our results offer clear evidence that TNAP modulates T lymphocyte function and specifically T cell-dependent colitis. PMID: 28039309
31. Data, including data from studies using cells from transgenic/knockout mice, suggest that Med1 plays role in enamel formation; Med1 induces Alpl via stimulation of Notch1 signaling by forming Notch1-RBP-Jk complex on Alpl promoter. (Med1 = mediator complex subunit 1; Alpl = alkaline phosphatase, liver-bone-kidney; Notch1 = Notch gene homolog 1; RBP-Jk = kappa J region recombining binding protein suppressor of hairless) PMID: 28673966
32. TNAP overexpression in vascular endothelium in mice leads to an unusual course of coronary atherosclerosis and was accompanied by the reduction in body weight and left ventricular ejection fraction. PMID: 29023576
33. intestinal alkaline phosphatase knockout mice display higher intestinal Ca uptake, which over time appears to correlate with a positive effect on the biomechanical properties of trabecular bone. PMID: 29234952
34. Study results from cerulein induced pancreatitis model in TNAP+/- mice show that altered TNAP expression results in heightened pancreatic inflammation, which may be explained by an augmented response of neutrophils and by a higher sensitivity of acinar cells to cerulein injury. PMID: 30251694

KEGG: mmu:11647

STRING: 10090.ENSMUSP00000030551

UniGene: Mm.288186