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Mini Sample Mouse Interleukin 1β,IL-1β ELISA Kit
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Mini Sample Mouse Interleukin 1β,IL-1β ELISA Kit
Sandwich quantitative immunoassay for interleukin 1β in mouse serum, plasma, cell culture supernates, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | interleukin 1β |
| Uniprot No. | P10749 |
| Species | Mus musculus (Mouse) |
| Sample types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection range | 12.5 pg/mL-800 pg/mL |
| Sensitivity | 6.25 pg/mL |
| Assay time | 3.5h |
| Sample loading volume | 50μL (The sample can be diluted by 2 times, requiring only 25μL.) |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Immunology |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
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In Stock : USA
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Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, interleukin 1β in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 12.5 pg/mL-800 pg/mL with a minimum detectable dose of 6.25 pg/mL.
This kit quantitatively measures mouse IL-1β, a master pro-inflammatory cytokine central to innate immune activation, inflammasome signaling, and fever response. Because IL-1β is often released in sharp, transient bursts following stimuli like LPS challenge or NLRP3 inflammasome activation, accurate capture across both baseline and induced conditions demands a carefully calibrated assay.
Detection Range: The 12.5–800 pg/mL range spans resting-state serum levels in naïve mice through the elevated concentrations typical of acute inflammatory models such as endotoxemia or colitis, minimizing the need for serial dilutions while covering most in vivo and in vitro experimental conditions.
- IL-1beta exerts variable effects on long-term potentiation at different kinds of synapses, indicating that IL-1beta has synapse-specific effects on hippocampal synaptic plasticity. PMID: 28637953
- Food-grade synthetic amorphous silica particles are able to directly initiate the endosomal MyD88-dependent pathogen pattern recognition and signaling pathway in steady-state dendritic cells. The ensuing activation of immature DCs results in de novo induction of pro-IL-1beta. PMID: 28645296
- we assessed the role of RIP3 in synergy with Caspase-1 in the induction of IL-1beta production in BMDM after either LPS/ATP or Chlamydia muridarum stimulation. The possibility of pyroptosis and necroptosis interplays and the role of RIP3 in IL-1beta production during Chlamydia muridarum infection in BMDM was investigated as well. PMID: 28660207
- These data reveal how, upon XIAP deficiency, a TLR-TNF-TNFR2 axis drives cIAP1-TRAF2 degradation to allow TLR or TNFR1 activation of RIPK3-caspase-8 and IL-1beta. This mechanism may explain why XIAP-deficient patients can exhibit symptoms reminiscent of patients with activating inflammasome mutations. PMID: 28723569
- identify interleukin-1 beta as an upstream trigger for the upregulation of interactions between USP5 and Cav3.2 channels in the pain pathway PMID: 28741432
- the present study confirmed that miR98 targeted the 3'untranslated region of Bcl2. In conclusion, miRNAcoordinated regulation of apoptosisassociated protein expression has been identified in Osteoarthritis chondrocytes following IL1beta induction. PMID: 28765925
- bone marrow-derived macrophages (BMM) and three murine macrophage cell lines, J774.1, J774A.1, and RAW264.7 were exposed to ATP or fibrous titanium dioxide (FTiO2) in the presence or absence of lipopolysaccharide (LPS), and the concentrations of IL-1beta and IL-6 in both cell lysates and in the culture media were measured by immunoblotting to differentiate active form of IL-1beta from pro-IL-1beta. PMID: 28766178
- This finding shows that autophagy and NLRP3 inflammasome activation are connected, and that PTPN22 plays a key role in the regulation of those 2 pathways. PMID: 28786745
- IL-33 may induce Th17 cell responses via IL-1beta and IL-6 derived from IL-33-matured dendritic cells. PMID: 28802996
- Mice treated with HW for 4 weeks demonstrated a significant decrease in the AD severity score compared with PW-treated mice (p less than 0.01). Hydrogen water administration also significantly reduced TEWL and serum TARC levels (p less than 0.01), infiltration of mast cells (p less than 0.05), and secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-33 (p less than 0.05) in skin lesions compared wit... PMID: 28889151
- ESP of fifth-stage larval Angiostrongylus cantonensis stimulates astrocyte activation and IL-1beta and IL-6 production through NF-kappaB and the Shh signaling pathway. PMID: 28950910
- In fibrocystin/polyductin complex-defective cholangiocytes, beta-catenin and IL-1beta are responsible for signal transducer and activator of transcription 3-dependent secretion of CXCL10 PMID: 29140564
- An OA model was established in mouse articular chondrocytes (MACs) treated by interleukin-1beta (IL-1beta). PMID: 29247798
- observation from the present research work reveals that Quercetin suppressed the production of proinflammatory cytokines at different levels, such as TNF-alpha and IL-1beta, and inhibits the activation of I-kappaB phosphorylation, whereas the total content was not affected. PMID: 29322353
- SAG2A differentially modulates IL-1beta expression in resistant and susceptible murine peritoneal macrophages cells. PMID: 29353306
- High IL-1beta expression is associated with experimental autoimmune encephalomyelitis. PMID: 29358392
- The present study demonstrated that IL-1b may induce ICAM-1 expression, thus enhancing the cohesion between mesenchymal stem cells and endothelial progenitor cells via the p38 MAPK signaling pathway. PMID: 29393395
- Alendronate (ALN)-augmented IL-1beta production and cell death require Smad3 and ASC activation, and SIS3 and anti-ASC antibodies may serve as palliative agents for necrotizing inflammatory diseases caused by ALN PMID: 29438662
- show that mutant KRAS facilitates IKKalpha-mediated responsiveness of tumor cells to host IL-1beta, thereby establishing a host-to-tumor signaling circuit that culminates in inflammatory MPE development and drug resistance PMID: 29445180
- These data suggest that amyloid formation leads to reduced PKB phosphorylation in beta-cells which is associated with elevated islet IL-1beta levels. Inhibitors of amyloid or amyloid-induced IL-1beta production may provide a new approach to restore phospho-PKB levels thereby enhance beta-cell survival and proliferation in conditions associated with islet amyloid formation PMID: 29474443
- These results suggest that mitochondrial ROS-TXNIP/NLRP3/IL-1beta axis activation is responsible for tubular oxidative injury, which can be ameliorated by MitoQ via the inhibition of mtROS overproduction PMID: 29475133
- urinary LRG is produced in renal tubular epithelial cells by interleukin-1beta (IL-1beta) that is released during proteinuria-induced renal damage PMID: 29550485
- HMGB1/IL-1beta complexes released after burn injuries can modulate immune responses PMID: 29601597
- Macrophage-derived IL1B/NF-kappaB signaling mediates parenteral nutrition-associated cholestasis in a mouse model. PMID: 29643332
- propofol was the most potent inhibitor of IL-1beta secretion and ROS level in S. aureus-infected RAW264.7 cells; moreover, propofol resulted in an increase in bacterial survival by inhibiting ROS and phagocytosis. PMID: 29667111
- Circadian clock protein BMAL1 regulates IL-1beta in macrophages via NRF2. PMID: 30127006
- fenretinide impaired proinflammatory cytokine interleukin 1 beta (IL-1beta) production in response to A. fumigatus exposure with contributions by lectin-type oxidized LDL receptor 1 (LOX-1) and c-Jun N-terminal kinase (JNK). PMID: 30211745
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
 | ||||||
pg/ml | OD1 | OD2 | Average | Corrected | ||
800 | 2.672 | 2.740 | 2.706 | 2.601 | ||
400 | 1.684 | 1.772 | 1.728 | 1.623 | ||
200 | 0.990 | 0.977 | 0.984 | 0.879 | ||
100 | 0.646 | 0.656 | 0.651 | 0.546 | ||
50 | 0.322 | 0.327 | 0.324 | 0.219 | ||
25 | 0.234 | 0.223 | 0.228 | 0.123 | ||
12.5 | 0.167 | 0.162 | 0.164 | 0.059 | ||
0 | 0.106 | 0.105 | 0.105 |
| ||
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 95 | ||||
Range % | 86-101 | |||||
1:2 | Average % | 92 | ||||
Range % | 88-100 | |||||
1:4 | Average % | 98 | ||||
Range % | 93-106 | |||||
1:8 | Average % | 91 | ||||
Range % | 87-98 | |||||
The recovery of mouse IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 83-94 | ||||
EDTA plasma (n=4) | 97 | 91-105 | ||||