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Human tissue inhibitors of metalloproteinase 3,TIMP-3 ELISA Kit
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Human tissue inhibitors of metalloproteinase 3,TIMP-3 ELISA Kit
Sandwich quantitative immunoassay for TIMP metallopeptidase inhibitor 3 in human serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | TIMP metallopeptidase inhibitor 3 |
| Uniprot No. | P35625 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 31.25 pg/ml-2000 pg/ml |
| Sensitivity | 7.8 pg/ml |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cancer |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, TIMP metallopeptidase inhibitor 3 in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 31.25 pg/ml-2000 pg/ml with a minimum detectable dose of 7.8 pg/ml.
The ELISA Kit is designed for quantitatively measuring human TIMP3 levels in samples, including serum, plasma, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of TIMP3 in samples. The color intensity is measured at 450 nm via a microplate reader.
TIMP3 has the broadest range of inhibition, targeting all members of the matrix metalloproteinase (MMP) family and several members of a disintegrin and metalloproteinases (ADAMs), and ADAM with thrombospondin motifs (ADAMTSs) family. Independent of its inhibitory capabilities, TIMP3 is also involved in promoting cell proliferation and regulating angiogenesis and apoptosis. The absence of TIMP3 results in poor cardiac remodeling and has been related to myocardial infarction or hypertension. In cancer studies, TIMP3 plays an important role in the cancer hallmark by controlling cell death, angiogenesis, tumor inflammation, and tumor cell invasion and dissemination.
- The expression level of LIPC, SLC16A8, and TIMP-3 was significantly associated with age-related macular degeneration pathology. PMID: 27966779
- Of the 225 genetic tests performed, 150 were for recessive IRD, and 75 were for dominant IRD. A positive molecular diagnosis was made in 70 (59%) of probands with recessive IRD and 19 (26%) probands with dominant IRD. Thirty-two novel variants were identified; among these, 17 sequence changes in four genes were predicted to be possibly or probably damaging including: ABCA4 (14), BEST1 (2), PRPH2 (1), and TIMP3 PMID: 28005406
- Single Nucleotide Variants of Candidate Genes in Aggrecan Metabolic Pathway Are Associated with Lumbar Disc Degeneration and Modic Changes PMID: 28081267
- Plasma TIMP3 is a biomarker for predicting the tumor stage in patients with oral squamous cell carcinoma . PMID: 28138307
- TIMP3 overexpression after myocardial infarction improves myocardial structural remodeling and function by promoting angiogenesis and inhibiting early proteolysis. PMID: 28550172
- Sphingosine-1-phosphate inhibited cell migration and MMP-2 expression through the upregulation of the tissue inhibitor of metalloproteinase-3 (TIMP-3) expression in human chondrosarcoma cells. PMID: 28672103
- Collectively, these results demonstrated that IL-32alpha upregulates the atheroprotective genes Timp3 and Reck by downregulating microRNA-205 through regulation of the Rprd2-Dgcr8/Ddx5-Dicer1 biogenesis pathway. PMID: 28740544
- TIMP-3 mRNA expression levels positively correlates with levels of miR-21 in in situ breast carcinomas and negatively in progesterone receptor positive invasive breast carcinomas. PMID: 28935174
- As a novel CLOCK-dependent diurnal gene, TIMP3 inhibits the expression of inflammatory cytokines that are up-regulated by UV irradiation in human keratinocytes. PMID: 29180440
- Preliminary studies indicate that baseline MMP3 and TIMP3 concentrations are associated with patient survival and disease-free time PMID: 29304854
- miR-21-5p mediates apoptosis by targeting PTEN and TIMP3. PMID: 29393355
- Genetic variation in the TIMP-3 gene may contribute to individual differences in mixture plaque susceptibility in the Han Chinese population. PMID: 29498555
- TIMP3 methylation is a marker for TN tumours and furthermore we showed for the first time that TIMP3 promoter methylation is an epigenetic marker of BRCA1ness tumours. PMID: 29524167
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||||||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||||||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||||||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||||||||||
| Intra-Assay Precision | Inter-Assay Precision | ||||||||||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 | ||||||||
n | 20 | 20 | 20 | 20 | 20 | 20 | ||||||||
Mean(pg/ml) | 246.660 | 246.505 | 242.560 | 248.749 | 245.363 | 251.011 | ||||||||
SD | 0.038 | 0.035 | 0.046 | 0.034 | 0.033 | 0.031 | ||||||||
CV(%) | 6.221 | 5.710 | 7.590 | 5.574 | 5.464 | 5.049 | ||||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||
| |||||||||||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||||||||||
2000 | 2.753 | 3.081 | 2.917 | 2.761 | |||||||||||
1000 | 1.636 | 1.796 | 1.716 | 1.560 | |||||||||||
500 | 1.030 | 1.088 | 1.059 | 0.903 | |||||||||||
250 | 0.541 | 0.565 | 0.553 | 0.397 | |||||||||||
125 | 0.390 | 0.365 | 0.378 | 0.222 | |||||||||||
62.5 | 0.261 | 0.247 | 0.254 | 0.098 | |||||||||||
31.25 | 0.202 | 0.215 | 0.209 | 0.053 | |||||||||||
0 | 0.154 | 0.157 | 0.156 | ||||||||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human TIMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||||
| Sample | Serum(n=4) | |||||||||||||
1:4 | Average % | 91 | |||||||||||||
Range % | 87-98 | ||||||||||||||
1:8 | Average % | 90 | |||||||||||||
Range % | 85-94 | ||||||||||||||
1:16 | Average % | 92 | |||||||||||||
Range % | 88-93 | ||||||||||||||
1:32 | Average % | 96 | |||||||||||||
Range % | 89-98 | ||||||||||||||
The recovery of human TIMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||||
Sample Type | Average % Recovery | Range% | |||||||||||||
Serum (n=5) | 91 | 87-94 | |||||||||||||
EDTA plasma (n=4) | 95 | 88-97 | |||||||||||||