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Human matrix metalloproteinase 3/stromelysin 1(MMP3/STR1) ELISA kit
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Human matrix metalloproteinase 3/stromelysin 1(MMP3/STR1) ELISA kit
Sandwich quantitative immunoassay for matrix metallopeptidase 3 (stromelysin 1, progelatinase) in human serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | matrix metallopeptidase 3 (stromelysin 1, progelatinase) |
| Uniprot No. | P08254 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 0.156 ng/mL-10 ng/mL |
| Sensitivity | 0.054 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cancer |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, matrix metallopeptidase 3 (stromelysin 1, progelatinase) in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 0.156 ng/mL-10 ng/mL with a minimum detectable dose of 0.054 ng/mL.
This Human MMP3 ELISA Kit is suitable for qualitatively determining MMP3 concentrations in multiple biological fluids, including human serum, plasma, and tissue homogenates in vitro. MMP3, a zinc-dependent protease, has broad substrate specificity and can degrade fibronectin, laminin, elastin, collagen IV, and proteoglycans of the extracellular matrix (ECM) in the systemic organs. MMP3 is involved in normal extracellular matrix turnover during embryonic development, organ morphogenesis, and wound healing, and in tissue destruction associated with aneurysms, cancer, arthritis, and heart failure. It is also associated with the pathogenesis of neurodegenerative diseases including Alzheimer's disease (AD) and Parkinson’s disease (PD). It has also been reported the important role of MMP3 as a signaling molecule in the neuronal apoptotic process as well as in neuroinflammation. This kit uses the quantitative sandwich-based enzyme immunoassay technique to measure the amount of human MMP3 in the sample. Standards and samples are respectively added to the microplate wells pre-coated with an anti-human MMP3 antibody. Biotin-labeled MMP3 antibody, HRP-avidin, and TMB substrate are pipped into the microplate in turn. The capture antibody pre-coated on the plate captures the MMP3 in the rat samples. MMP3 binds to the biotinylated anti-human MMP3 monoclonal antibody. And the biotin on the biotinylated anti-human MMP3 monoclonal antibody binds to the avidin on the enzyme label, forming immune complexes. The color renders blue after the addition of the TMB substrate. The addition of the stop solution into the wells immediately turns the blue into yellow. The concentration of MMP3 in the samples is directly proportional to OD (450nm). Each manufactured lot of this ELISA kit was quality tested for criteria such as sensitivity, specificity, precision, linearity, and lot-to-lot consistency.
- Matrix metalloprotease (MMP) regulation was the top pathway involved in gingival aging. MMP3, MMP9, MMP12, and MMP13 were upregulated in old gingival tissues, concomitantly with interleukin-1 beta (IL1B) expression. PMID: 27391467
- Study showed that serum MMP-3 levels are decreased in patients with epilepsy, which is relevant for acute stroke. A specific pathogenic effect of MMP-3 in epilepsy appears unlikely. This study has demonstrated that the newly developed Luminex assays are useful in quantifying MMP-3 levels in human serum. PMID: 27423606
- MMP-3 positivity has a strong relationship with meningiomas having an aggressive character; MMP-3 may be used as a proliferation marker for biological behaviour, recurrence rate and prognosis of meningiomas PMID: 27438616
- In summary, our study demonstrates that IL-7/IL-7R axis promotes the invasion and migration of prostate cancer cells, through activation of AKT/NF-kappaB pathway and upregulation of MMP-3 and MMP-7 expression PMID: 27611862
- 5A5A genotype of MMP-3-1171 5A/6A gene polymorphism was associated with adolescent idiopathic scoliosis, especially in Caucasian population. (Meta-analysis) PMID: 27659327
- MMP-3 -11715A/6A polymorphism was associated with MMP-3 expression in herniated disc tissues. MMP-3 expression and the histological and radiological scores were positively correlated. PMID: 27706715
- MMP-3 expression is deregulated in osteosarcomas and this potentially contributes to metastasis and might be a promising marker for the prognosis and therapy of metastatic osteosarcoma. PMID: 27837634
- promoter polymorphism rs3025058 associated with adolescent idiopathic scoliosis but not with gender bias PMID: 27911281
- We concluded that overexpression of MMP-3 and uPA, altogether with diminished expression of PAI-1 from metastatic tumors, might be a crucial step towards metastasis in ductal breast cancer. PMID: 27975070
- NFAT1 silencing could suppress cell migration and invasion through MMP-3. PMID: 28024290
- These results indicate that periodic induction, via use of an eye drop, of AAV-mediated secretion of MMP-3 into aqueous humour could have therapeutic potential for those cases of glaucoma that are sub-optimally responsive to conventional pressure-reducing medications. PMID: 28158775
- Positive MMP-3 was not significantly related to osteoporosis or severe hand osteoarthritis. PMID: 28365812
- These results, for the first time, demonstrate that P15 binding to cell surface vimentin inhibits the tumor cell invasion and is associated with reduced MMP3 expression. Thus, suggesting that P15 has potential as an anti-metastatic therapy in pancreatic cancer. PMID: 28396463
- In patients with ankylosing spondylitis, serum MMP3 level was found to have a positive correlation with the MRI score of sacroiliac joint and c-reactive protein. PMID: 28432524
- among Chinese males, MMP-3 rs650108 and MMP-8 rs2012390 decrease alcohol-induced ONFH risk and MMP-8 rs11225394 increases it. PMID: 28445942
- results demonstrated that measurement of MMP-3 could become a marker of disease activity in rheumatoid arthritis patients PMID: 28461705
- study to explore the relationship between 2 polymorphisms (MMP-1-755 T/G [rs498186] and MMP-3 A/C [rs632478]) and disc degeneration; a significant association was found between the MMP-3 polymorphism and disc degeneration; the homozygote CC was associated with an increased risk of disc degeneration compared with the AA genotype PMID: 28497435
- Report an association between the MMP-3 rs3025058 and subclinical markers of carotid and coronary atherosclerosis at the time of recruitment. MMP-3 rs3025058 on affects carotid artery disease progression in the 3.8-year follow-up in patients with T2DM. PMID: 28521653
- A Study of IL-1beta, MMP-3, TGF-beta1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population PMID: 28676856
- These observations suggest a crucial role for cancer-associated fibroblasts and fibroblast growth factor-1/fibroblast growth factor receptor 4 signaling in the progression of ovarian cancer. the expression level of Snail1 and MMP3 was reduced, while the expression level of E-cadherin increased PMID: 28718374
- The 5A allele of the MMP3-gene promoter polymorphism is a potential risk factor in the poor outcome of hemodialysis patients. PMID: 28781337
- No interaction between MMP-3 5A/6A polymorphism and the risk of recurrence in total IS patients was found. The variant 5A/6A+5A/5A genotype and the 5A allele were significantly associated with a high risk of recurrence for large-artery atherosclerosis (LAA) (multivariate-adjusted, P=0.002, 0.001, respectively), but not for small-artery occlusion and cardioembolism. PMID: 28843428
- Suppression of active MMP3, but not total circulating MMP3 was associated with treatment response in rheumatoid arthritis patients treated with tocilizumab. PMID: 28850021
- A significant increase in the frequency of the MMP3-1171dupA (rs3025058) polymorphism genotype among patients with unstable angina was detected. PMID: 29044936
- MMP3 (and MMP-1 and MMP-2) are independently associated with markers of arterial stiffening in patients with type 1 diabetes. PMID: 29070037
- Data suggest that serum matrix metalloproteinase-3 (MMP-3) and the 7-joint ultrasound score (US7) scores could both effectively reflect disease activity and therapeutic responses in patients with moderate to severe rheumatoid arthritis (RA). PMID: 29141665
- Higher serum MMP-3 levels in knee osteoarthritis reflect disease "generalization". PMID: 29164307
- C/EBPbeta upregulation promoted tumor cell invasion in an MMP3-dependent manner in vitro and was associated with metastatic status in colorectal cancer PMID: 29172775
- BMAL1 Deficiency Contributes to Mandibular Dysplasia by Upregulating MMP3. PMID: 29276151
- Preliminary studies indicate that baseline MMP3 and TIMP3 concentrations are associated with patient survival and disease-free time PMID: 29304854
- MMP-3 (Lys45Glu) polymorphisms associate with obesity risk and its severity. PMID: 29317790
- studies establish a molecular imaging reporter for diagnosing early-stage EOC. Additional studies are required to confirm the early-stage activity of MMP-3 in EOC and its diagnostic and prognostic significance PMID: 29390034
- Report a positive correlation between glomerular filtration rate and MMP-3 activity in diabetic patients. PMID: 29421776
- rs3025058 in MMP3 and rs2276109 in MMP12 might not contribute to the risk of developing rheumatic heart disease in a Han population in Southern China. PMID: 29458338
- The maternal and fetal MMP3 gene polymorphisms may be strong genetic markers of preeclampsia, occurring either individually or together. PMID: 29670668
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
