The Human hepatitis B virus e antigen (HBeAg) ELISA kit is used for qualitative identification of HBeAg in human serum and plasma. It employs the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with HBeAb. Samples or standards are pipetted into the wells with HRP-conjugated HBeAb. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of HBeAg bound in the initial step. The color development is stopped and the intensity of the color is measured by a microplate reader at 450 nm. It indicates the presence of HBeAg if the O.D. (optical density) of HBeAg is greater than or equal to the cutoff value (2.1× Average O.D. value of negative control). There is no HBeAg present in the sample if the O.D. is less than the cutoff value. This assay has high sensitivity and excellent specificity for detection of HBeAg. And it also has been validated with precision less than 20% and lot-to-lot consistency. Get more details from the product instructions. HBeAg is an HBV's non-particulate protein derived from its pre-core/core precursor after proteolytic processing. A variety of animal studies have demonstrated that HBeAg does not participate in viral infection, replication, and assembly, but is vital for natural infection in Vivo. HBeAg is used as a marker of infectivity and has a tolerogenic and immunomodulatory activity that plays a remarkable role in viral persistence. HBeAg is the exclusive HBV protein to cross the placenta to thus establish neonatal T cell tolerance to both HBeAg and HBcAg. Chronic hepatitis B (CHB) patients diagnosed negative in HBeAg is difficult to treat and have an increased risk of cirrhosis and hepatocellular carcinoma (HCC). Most patients after HBeAg seroconversion become "inactive HBsAg carriers".

• A 96-well Assay plate --The 96-well plate has been pre-coated with HBeAb.
• Negative Control (1 x 1ml) --Eliminate false positive
• Positive Control (1 x 1ml) --Used to evaluate the validity, stability, and comparability of experimental results.
• HRP-conjugated HBeAb(1 x 7ml) --Bind to the HBeAg, and HRP catalyzes the TMB to elicit a chromogenic reaction.
• Wash Buffer (20x concentrate) (1 x 30 ml) --Wash away unbound or free substances.
• Substrate A (1 x 7 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
• Substrate B (1 x 7 ml) --Mix with substrate A and interact with HRP, eliciting a Chromogenic reaction.
• Stop Solution (1 x 7 ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells)--Cover the microplate when incubation.
• An Instruction manual

• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
• An incubator that can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution