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Human Tissue Inhibitor Of Matrix Metalloprotease-1 (TIMP-1) ELISA KIT
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Human Tissue Inhibitor Of Matrix Metalloprotease-1 (TIMP-1) ELISA KIT
Sandwich quantitative immunoassay for TIMP metallopeptidase inhibitor 1 in human serum, plasma, tissue homogenates available in multiple catalog sizes:
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| Target name | TIMP metallopeptidase inhibitor 1 |
| Uniprot No. | P01033 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 0.39 ng/mL-25 ng/mL |
| Sensitivity | 0.434 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cardiovascular |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, TIMP metallopeptidase inhibitor 1 in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 0.39 ng/mL-25 ng/mL with a minimum detectable dose of 0.434 ng/mL.
The human tissue inhibitor of matrix metalloprotease-1 (TIMP1) ELISA kit is suitable for the quantitative determination of human TIMP1 in serum, plasma, or tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify human TIMP1 levels in the sample. The amount of synthesized colored product is positively related to the analyte of interest in the sample.
TIMP1 is a glycoprotein produced by cardiac myocytes and fibroblasts in the normal heart. TIMP1 binds with active MMPs to inhibit MMP activity. In addition to suppressing MMP activity, TIMP1 also regulates multiple biological activities, including inhibiting apoptosis, stimulating cell proliferation and differentiation, as well as promoting migration and angiogenesis or the metastatic potential of tumor cells. Overexpression of TIMP1 has been found in several types of human cancers, including prostate cancer, lung cancer, melanoma, glioblastoma, and breast cancer. TIMP1 acting as a specific hallmark of human cancer is also associated with the tumor microenvironment (TME) and drug resistance.
- This study indicates that in our population, the COL4A3 rs55703767 polymorphism decreased the risk of KC. However, the TIMP-1 rs6609533 polymorphism was associated with an increased risk of KC. PMID: 28197741
- Low MMP-8/TIMP-1 reflects left ventricle impairment in takotsubo cardiomyopathy and high TIMP-1 may help to differentiate it from acute coronary syndrome PMID: 28278213
- miR-516a-5p may regulate MTHFR, MMP-2, and TIMP-1 expressions in vascular smooth muscle cells, possibly promoting the disruption of homocysteine metabolism and proteolytic degradation of elastin for abdominal aortic aneurysm formation. PMID: 28288890
- Results suggest a crucial role of MMP9 at the early stage of carcinogenesis in the large intestine. The increase in MMP9 and TIMP1 mRNA concentration and the decrease in MMP28 in the large intestinal tissue may be a confirmation of cancer. PMID: 28293015
- Suggest that IL-6 could promote the invasiveness of breast cancer cells by inducing secretion of TIMP-1 and -2, causing a disturbance in TIMP/MMP balance. PMID: 28375112
- In III-rd trimester of pregnancy parameters of Timp-1 and Survivin - anti-apoptotic substances concentration were similar in maternal and cord blood in both artery and vein. We found no increased activity of selected antiapoptotic factors. PMID: 28509321
- We conclude that in the resected esophageal cancer an increased mRNA expression of MMP-7, MMP-10 and TIMP-1 correlated with clinicopathologic features. We suggest that these genes may play a role during progression of the diseaseMMP-10, MMP-7, TIMP-1, TIMP-2 were overexpressed in 73%, 85%, 55% and 42% of esophageal cancer samples, respectively. PMID: 28510611
- C-type natriuretic peptide (CNP)administration significantly decreased the secretion and expression of MMP-2 and MMP-9 in the cultured mesangial cells ; (2) the secretion and expression of TIMP-1 progressively elevated after treatment with CNP for 24 and 48 h, whereas declined at later time point; (3) CNP expression was negatively correlated with MMP-2 and MMP-9 expression. PMID: 28554303
- no significant difference was detected in the expression level of individual MMP9 and TIMP1 genes in SCZ patients versus healthy controls either in total numbers of subject or in sex based subgroups PMID: 28578515
- Increased expression of TIMP1 (for both mRNA and protein) associated with poor ulcer healing in Diabetic Foot Ulcers with infection. PMID: 28682675
- corticosteroid-resistant patients have higher basal MMP-9 levels and produce more MMP-9 levels in response to IL-1beta. PMID: 28696822
- the key role of TIMP-1 for the anti-angiogenic properties of stimulated MSC secretome already observed in mouse is confirmed in human. PMID: 28739509
- Tissue inhibitor of matrix metalloproteinases 1 (TIMP1) inhibition resensitized tumors to gemcitabine and radiotherapy. PMID: 28765154
- Study found that TIMP-1 accelerates cell proliferation through YAP/TAZ activation in cancer. PMID: 28925394
- Gal-3 gene and protein expression levels were also significantly higher in lung tissue from an allergic asthma murine model using TIMP-1 knockout. PMID: 28992358
- Significant differences in gene expression profiles were observed between patients with post-kidney transplant bladder tumors and those with conventional bladder tumors. TIMP-l expression was significantly higher in first group than in the second. PMID: 29038419
- It is well established by now that progression of melanoma depends on ECM remodeling, TIMPs family being one of the most important regulators of this process. PMID: 29250646
- Authors observed a significant increase in the immunoexpression of collagen I and III in patients with DCM and a significant reduction in the immunoexpression of MMP-1 compared with the control group. Also, the collagen I and TIMP-1 expression indicated a positive linear correlation and respectively a negative linear relationship with collagen III and MMP-1. PMID: 29250654
- Reduced expression of types I and III collagen and TIMP-1 as well as the increased expression of MMP-1 and MMP-8 in the anterior vaginal wall tissues play important roles in the onset of pelvic organ prolapse. PMID: 29263043
- female patients exhibited lower methylation levels of MMP-9 but higher methylation levels of TIMP-1 compared with male patients, and the methylation levels of TIMP-1 gradually decreased with age. PMID: 29298087
- plasma levels of TIMP-1 are associated with pancreatic lesion-induced cachexia in patients without jaundice. PMID: 29394913
- IL-33 can regulate deposition of ECM and promote the process of pulmonary fibrosis by inducing the imbalance between MMP-9 and TIMP-1. PMID: 29417309
- In dilation cardiomyopathy, expression of MMP-2, MMP-9, and TIMP-1 and their ratios in autopsy material and in cultures was elevated by 1.5-9 times. PMID: 29504111
- High Co-expression of TIMP-1 and CD63 is associated with glioblastoma. PMID: 29523123
- Results found that CK18, MMP-9, and TIMP1 averages of those with positive clinical lymph nodes and those in clinical stage 3 were higher than the averages of those with negative clinical lymph nodes and those in clinical stage 2. PMID: 29651326
- that TLR2 is involved in IL29-stimulated TIMP1 expression in Raw264.7 cells and primary macrophages. PMID: 29658577
- Increased matrix metalloproteinase-9/tissue inhibitor of metalloproteinase-1 ratio in smokers with airway hyperresponsiveness is associated with accelerated lung function decline. PMID: 29692608
- significantly increased in the serum of type 2 diabetes mellitus patients with diabetic retinopathy PMID: 29752166
- In monocytes, incubation with psoriasis' sera increased the production of MMP-9 and TIMP-1 in comparison with both baseline and monocytes incubated with non-psoriatic sera PMID: 29979898
- MMP-9 and TIMP-1 levels are correlated with age, pathological grade, tumor size and lymph node metastasis of breast cancer patients. PMID: 30010105
- Study evaluated for the first time the plasma levels and the diagnostic usefulness of VEGF, MMP-9, and TIMP-1 in cervical cancer (CC), not only independently but especially in combination with established cervical tumor markers. All tested parameters showed statistical significance when compared their concentrations in patients with CC to healthy women. PMID: 30037277
- Low TIMP1 expression is associated with cervical cancer. PMID: 30052166
- no difference in the expression of MMP-7 and TIMP-1 in the endometrium in relation to hormone levels and menstrual cycle phases were observed PMID: 30187906
- High serum tissue inhibitor of metalloproteinase-1 level is associated with gastric cancer. PMID: 30192205
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
To assess the linearity of the assay, samples were spiked with high concentrations of human Timp-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
