Testosterone is a steroid hormone primarily produced in the testes in males and ovaries in females, with smaller amounts synthesized in the adrenal glands. As the principal male sex hormone, testosterone plays important roles in the development of male reproductive tissues, secondary sexual characteristics, and maintenance of muscle mass, bone density, and red blood cell production. In research contexts, testosterone measurement is necessary for studying endocrine function, reproductive health, aging processes, and various pathological conditions including hypogonadism, polycystic ovary syndrome, and metabolic disorders. The Human Testosterone ELISA Kit (CSB-E05099h) provides a quantitative competitive immunoassay for measuring testosterone levels in human samples. This kit operates on a competitive measurement principle and requires sample volumes of 50-100 microliters. The assay can be completed within 1-5 hours. Detection occurs at 450 nm wavelength. Researchers should request specific information regarding the detection range and sensitivity parameters for this particular kit. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in research studies to quantify testosterone levels in human biological samples, supporting studies of endocrine function and hormonal regulation. The kit has been applied to analyze both serum and plasma samples, allowing researchers to measure testosterone concentrations across different study contexts. Applications have included multi-analyte profiling studies where testosterone measurements are combined with inflammatory cytokine measurements. • Endocrine research: Measurement of testosterone levels in human serum and plasma samples to study hormonal status and endocrine function • Multi-biomarker studies: Integration of testosterone quantification with inflammatory cytokine profiling to examine relationships between hormonal and immune system parameters • Comparative analysis: Measurement of testosterone concentrations alongside other biomarkers in peripheral blood samples from human subjects • Tissue analysis: Measurement of testosterone levels in processed tissue samples using homogenate preparations