Tumor necrosis factor receptor superfamily member 11b (TNFRSF11B), commonly known as osteoprotegerin (OPG), is a regulatory protein in bone metabolism and immune system function. As a decoy receptor for RANKL (receptor activator of nuclear factor kappa-B ligand), OPG blocks osteoclast differentiation and activation, preventing excessive bone resorption. This glycoprotein maintains bone homeostasis and has been linked to various pathological conditions including osteoporosis, rheumatoid arthritis, and certain malignancies. Research into OPG levels offers insights into bone turnover dynamics and inflammatory processes. The Human Osteoprotegerin ELISA Kit (CSB-E04692h) uses a quantitative sandwich measurement principle for detecting TNFRSF11B in human samples. This assay works with serum, plasma, and tissue homogenates with a detection range of 7.8 pg/mL to 500 pg/mL and shows sensitivity down to 1.95 pg/mL. The protocol needs 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in research studying bone metabolism and inflammatory processes, particularly in studies examining the relationship between respiratory disease and bone health. The kit allows quantification of osteoprotegerin in serum samples from clinical populations and cell culture supernatants from in vitro experiments. • Respiratory disease research: Applied in studies comparing patients with chronic obstructive pulmonary disease to healthy controls, examining bone mineral density relationships and inflammatory marker profiles • Bone metabolism studies: Used to measure osteoprotegerin levels in osteoblast cell culture supernatants following treatment with bone-targeting compounds and growth factors • Clinical biomarker analysis: Applied for serum osteoprotegerin quantification in patient populations with varying bone mineral density status compared to healthy volunteers • Cell culture applications: Used in controlled in vitro experiments to assess osteoprotegerin production by cultured osteoblasts under different experimental conditions

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Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.